Preparation and optimisation of liposome‐in‐alginate beads containing oyster hydrolysate for sustained release

Oyster (Crassostrea gigas) hydrolysate shows antihypertensive effect in our previous study. Oral administration of oyster hydrolysate can loss bioactive peptides due to enzymatic degradation in vivo. To maximise its bioavailability, liposome‐in‐alginate (LA) beads were used to encapsulate the oyster hydrolysates to protect from degradation and obtain sustained release. The preparation conditions of the LA beads were optimised by response surface method using a model peptide of tyrosylalanine (YA). Their characterisation, swelling and release properties were investigated. The optimised conditions for the concentration of calcium chloride, sodium alginate and the amount of ethanol‐dissolved lecithin (EDL) were 0.5 m , 3% and 95.4 mg, respectively. The encapsulation efficiencies of YA and the oyster hydrolysate in the optimised condition were 74.9% and 84.3%, respectively. The release time of the oyster hydrolysate in the simulated gastrointestinal fluid was up to 16 h. The LA beads can be recommended to encapsulate oyster hydrolysate for bioavailability improvement.     

Thermal analysis of 9977 radioactive material package

The 9977 package is a certified type B transportation packaging that was designed to transport radioactive materials with a decay heat load of up to 19?W. The packaging was recently modified to accommodate increased content heat load (up to 38?W) by employing an aluminium heat dissipating sleeve outside the containment vessel (CV), as well as an aluminium spacer inside the CV holding two 3013 containers. This paper provides highlights of thermal analyses of the modified 9977 package that were performed to evaluate its compliance with the 10 CFR 71 regulatory safety requirements. Parametric studies were also performed to examine effects of (i) surface properties, e.g. light absorptivity and emissivity, of the packaging; (ii) total decay heat loads, ranging from 18 to 38?W; and (iii) distribution of decay heat load inside the CV on the temperature of the Viton O-ring seal. The results of thermal analyses show that for the normal condition of transport with insolation, increasing absorptivity and decreasing emissivity increase the temperature of the O-ring; decreasing heat load decreases the temperature of the O-ring, whereas changing heat load distribution has little effect on the temperature of the O-ring. Likewise, changing the thermal conductivity of the spacer inside the CVhas little effect on the temperature of the O-ring. Briefly discussed is the possible extension of the annual maintenance interval of the 9977 package, based on previous work and the data obtained in the long-term leak performance tests conducted by the Savannah River National Laboratory.     

Development of an embedded solar tracker for the enhancement of solar energy utilization

An embedded two‐axis solar tracking system using Laboratory Virtual Instrumentation Engineering Workbench to write the operation and control algorithms was developed for enhancing solar energy utilization. The system consists of a real‐time processor, two motion‐control modules, two step drives, two step motors, feedback devices, and other accessories needed for functional stability. The real‐time processor allows the solar tracker to be used as a stand‐alone, real‐time system that can operate automatically without any external control. The system combines two different solar tracking methods: the optical method and the astronomical method. Cadmium Sulfide (CdS) sensors are employed to continuously generate feedback signals to the controller, ensuring high‐precision solar tracking even under adverse conditions. The CdS sensor is a resistor whose electric resistance decreases with increasing incident light intensity. A database of solar altitude, azimuth, and sunrise and sunset times is provided by this solar tracking system. Other solar trackers operating in an astronomical method may access and use this database over the Internet. Solar position and sunrise and sunset times in the database were compared with those of the Astronomical Applications Department of the U.S. Naval Observatory. The differences were found to be negligible. Copyright © 2011 John Wiley & Sons, Ltd.     

Analytical method for fast screening and confirmation of multi-class veterinary drug residues in fish and shrimp by LC-MS/MS

A multi-class, multi-residue analytical method based on LC-MS/MS detection was developed for the screening and confirmation of 28 veterinary drug and metabolite residues in flatfish, shrimp and eel. The chosen veterinary drugs are prohibited or unauthorised compounds in Korea, which were categorised into various chemical classes including nitroimidazoles, benzimidazoles, sulfones, quinolones, macrolides, phenothiazines, pyrethroids and others. To achieve fast and simultaneous extraction of various analytes, a simple and generic liquid extraction procedure using EDTA-ammonium acetate buffer and acetonitrile, without further clean-up steps, was applied to sample preparation. The final extracts were analysed by ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS). The method was validated for each compound in each matrix at three different concentrations (5, 10 and 20 ng g^–1) in accordance with Codex guidelines (CAC/GL 71-2009). For most compounds, the recoveries were in the range of 60–110%, and precision, expressed as the relative standard deviation (RSD), was in the range of 5–15%. The detection capabilities (CCβs) were below or equal to 5 ng g^–1, which indicates that the developed method is sufficient to detect illegal fishery products containing the target compounds above the residue limit (10 ng g^–1) of the new regulatory system (Positive List System – PLS).     

Enzymatic synthesis of low-calorie structured lipids in a solvent-free system

For the synthesis of low-calorie structured lipids (LCSL), transesterification between triacetin and stearic acid using immobilized lipase in a solvent-free system was investigated. Stearic acid, a long-chain saturated fatty acid, was incorporated mainly into the sn-1 and/or sn-3 positions of triacetin by lipase-catalyzed reaction. Three types of reactor systems (open, closed, and vacuum) were studied for the production of LCSL. The effects of various reaction variables such as water activity of substrates and lipase, molar ratio of substrates, stirring speed and reaction temperature were investigated. In the vacuum reactor system, a certain amount of water was added periodically to maintain the optimal water content of the reaction system. When a suitable amount of water (0.65 wt% of substrates) was added at every 1 h into the vacuum reactor system, more than 88% LCSL was obtained within 4 h using Chirazyme^® L-2.     

The ability of zinc to inhibit the sporulation and viability of Clostridium sporogenes and growth of other bacteria

The objective of this study was to investigate the influence of zinc on the sporulation and viability of Clostridium sporogenes and on the growth of other bacteria. When 0.5% ZnCl₂ was added to a sporulation medium, it completely inhibited C. sporogenes (PA 3679) sporulation for up to 3 weeks. At concentrations of 0.5% and 1.0%, ZnCl₂ not only completely inactivated the vegetative cell viability (>7.0 Log reduction) but also significantly reduced the spore viability (<2.1 Log reduction) of C. sporogenes. Taken together, it was concluded that zinc blocks C. sporogenes sporulation by damaging (or killing) vegetative cells and probably by interfering with the biosynthesis of spore components. In addition to the inhibitory effect on the sporulation and viability of C. sporogenes, ZnCl₂ was found to have a broad antimicrobial spectrum against all Gram‐positive and Gram‐negative spoilage and pathogenic bacteria tested. The minimal inhibitory concentration for inhibiting the bacteria ranged between 3.7 and 7.4 mm . Therefore, we expect that this compound or a combination thereof has a potential as a surface‐cleaning agent or disinfectant.     

Modeling heterogeneous bacterial populations exposed to antibiotics: The logistic‐dynamics case

In typical in vitro tests for clinical use or development of antibiotics, samples from a bacterial population are exposed to an antibiotic at various concentrations. The resulting data can then be used to build a mathematical model suitable for dosing regimen design or for further development. For bacterial populations that include resistant subpopulations—an issue that has reached alarming proportions—building such a model is challenging. In prior work, we developed a related modeling framework for such heterogeneous bacterial populations following linear dynamics when exposed to an antibiotic. We extend this framework to the case of logistic dynamics, common among strongly resistant bacterial strains. Explicit formulas are developed that can be easily used in parameter estimation and subsequent dosing regimen design under realistic pharmacokinetic conditions. A case study using experimental data from the effect of an antibiotic on a gram‐negative bacterial population exemplifies the usefulness of the proposed approach. © 2015 American Institute of Chemical Engineers AIChE J, 61: 2385–2393, 2015     

Deformation and Fracture of β‐Silicon Nitride Micropillars

Room‐temperature deformation and fracture behaviors under microcompression of single crystal β‐silicon nitride (Si₃N₄) micropillars were investigated. Pillars were fabricated by focused ion beam (FIB) in large Si₃N₄ grains, located close to the basal and prismatic orientations, selected by electron backscatter diffraction (EBSD). The micromechanical test and the damage characterization were performed by nanoindentation and scanning electron microscopy (SEM), respectively. The elastic, plastic, and fracture properties, such as Young's modulus, yield stress, and rupture stress are considerably influenced by the pillar orientation. The activation of the type slip system was identified in case of basal oriented micropillars.