The yeast spindle pole body component Spc72p interacts with Stu2p and is required for proper microtubule assembly

We have previously shown that Stu2p is a microtubule-binding protein and a component of the Saccharomyces cerevisiae spindle pole body (SPB). Here we report the identification of Spc72p, a protein that interacts with Stu2p. Stu2p and Spc72p associate in the two-hybrid system and can be coimmunoprecipitated from yeast extracts. Stu2p and Spc72p also interact with themselves, suggesting the possibility of a multimeric Stu2p-Spc72p complex. Spc72p is an essential component of the SPB and is able to associate with a preexisting SPB, indicating that there is a dynamic exchange between soluble and SPB forms of Spc72p. Unlike Stu2p, Spc72p does not bind microtubules in vitro, and was not observed to localize along microtubules in vivo. A temperature-sensitive spc72 mutation causes defects in SPB morphology. In addition, most spc72 mutant cells lack cytoplasmic microtubules; the few cytoplasmic microtubules that are observed are excessively long, and some of these are unattached to the SPB. spc72 cells are able to duplicate and separate their SPBs to form a bipolar spindle, but spindle elongation and chromosome segregation rarely occur. The chromosome segregation block does not arrest the cell cycle; instead, spc72 cells undergo cytokinesis, producing aploid cells and polyploid cells that contain multiple SPBs.     

Self-in-relation theory and the role of the clinical nurse specialist. Part II: Application to advanced nursing roles in a professional practice model

CONCEPTS AND ASSUMPTIONS of self-in-relation theory of women's development, as proposed by feminist scholars at the Stone Center in Wellesley, Massachusetts, are applied in this article. This theory was used as a framework to guide staff development and mentorship roles of advanced practice nurses. How the theory served to direct these roles and assisted in development of a professional practice model is discussed. Exemplars from the authors' practices and professional practice outcomes after 2 years of theory implementation are presented.     

Studies of the neutralizing activity and avidity of anti-human immunodeficiency virus type 1 Env antibody elicited by DNA priming and protein boosting

DNA vaccination is an effective means of eliciting strong antibody responses to a number of viral antigens. However, DNA immunization alone has not generated persistent, high-titer antibody and neutralizing antibody responses to human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein (Env). We have previously reported that DNA-primed anti-Env antibody responses can be augmented by boosting with Env-expressing recombinant vaccinia viruses. We report here that recombinant Env protein provides a more effective boost of DNA-initiated antibody responses. In rabbits primed with Env-expressing plasmids, protein boosting increased titer, persistence, neutralizing activity, and avidity of anti-Env responses. While titers increased rapidly after boosting, avidity and neutralizing activity matured more slowly over a 6-month period following protein boosting. DNA priming and protein immunization with HIV-1 HXB-2 Env elicited neutralizing antibody for T cell line-adapted, but not primary isolate, viruses. The most effective neutralizing antibody responses were observed after priming with plasmids which expressed noninfectious virus-like particles. In contrast to immunizations with HIV-1 Env, DNA immunizations with the influenza virus hemagglutinin glycoprotein did not require a protein boost to achieve high-titer antibody with good avidity and persistence.     

小波变换在手写体汉字识别中的应用

汉字识别中的结构类特征具有抗变形的优点，合适做为手写体识别过程使用，但其中笔划特征的提取非常困难。本文用小波变换这一图象分析中有力工具对汉字图象分析处理之后，再进行笔划的提取，其过程简洁、明晰。     

电视监控系统云台控制器设计

电视监控系统是一种典型的分布式计算机信号采集系统。为了提高监控的范围和效果,云台控制器的优劣直接影响监控的范围和效果,因此对云台控制器的设计具有重要的意义。本系统采用串型通信,通过Modbus协议把控制信号输入单片机,经过对AT89S52单片机的编程,从而对八路继电器输出进行控制。系统不仅结构简单,而且还可以方便的对镜头、防护罩的控制进行扩展,功能强大,可以广泛应用于电视监控系统中的云台控制。     

FY-3气象卫星MERSI数据快速预处理的IDL实现

作为新型卫星数据源,FY\|3/MERSI(风云三号中分辨率光谱成像仪)影像的快速预处理方法与模块目前较少见。采用基于三角网的几何校正算法,根据研究区Shapefile文件和FY\|3/MERSI自带定位数据提取处理区域,实现对应区域全部20个通道几何校正,并利用后向映射重采样输出各通道图像纠正后像元点DN值,随后进行辐射定标、太阳高度角订正等预处理。该系列过程用IDL编写相关程序实现了用户界面化交互操作。运行结果表明:设计的算法流程占用内存小,计算速度快。对于一个面积约45万km²的区域,所有通道处理耗时仅450 s,且校正后影像质量和精度均较好。相较于相同区域借助ENVI软件下地理位置查找表法(GLT)仅实现单通道几何校正耗时超30 min,本文开发的模块大大提高了FY\|3/MERSI数据处理效率,为其在城市热环境、积雪等领域的应用做好准备工作。     

海量数据库迁移与升级

目前数据库中数据越来越多,应用对数据库在线时间的要求也越来越高,使得数据库迁移和数据库升级成为了一件非常困难的事情.为此,介绍了几种迁移和升级的方法,用以实现以尽可能少的停机时间对TB级数据库完成数据库的迁移和升级.