cPCN-Regulated SnO2 Composites Enables Perovskite Solar Cell with Efficiency Beyond 23％

Efficient electron transport layers(ETLs)not only play a crucial role in promoting carrier separation and electron extraction in perovskite solar cells(PSCs)but...     

Superhydrophobic Paper-Based Microfluidic Field-Effect Transistor Biosensor Functionalized with Semiconducting Single-Walled Carbon Nanotube and DNAzyme for Hypocalcemia Diagnosis

Hypocalcemia is caused by a sharp decline in blood calcium concentration after dairy cow calving, which can lead to various diseases or even death. It is necessary to develop an inexpensive, easy-to-operate, reliable sensor to diagnose hypocalcemia. The cellulose-paper-based microfluidic field-effect biosensor is promising for point-of-care, but it has poor mechanical strength and a short service life after exposure to an aqueous solution. Octadecyltrichlorosilane (OTS), as a popular organosilane derivative, can improve the hydrophobicity of cellulose paper to overcome the shortage of cellulose paper. In this work, OTS was used to produce the superhydrophobic cellulose paper that enhances the mechanical strength and short service life of MFB, and a microfluidic field-effect biosensor (MFB) with semiconducting single-walled carbon nanotubes (SWNTs) and DNAzyme was then developed for the Ca²⁺ determination. Pyrene carboxylic acid (PCA) attached to SWNTs through a non-covalent π-π stacking interaction provided a carboxyl group that can bond with an amino group of DNAzyme. Two DNAzymes with different sensitivities were designed by changing the sequence length and cleavage site, which were functionalized with SPFET/SWNTs-PCA to form Dual-MFB, decreasing the interference of impurities in cow blood. After optimizing the detecting parameters, Dual-MFB could determine the Ca²⁺ concentration in the range of 25 μM to 5 mM, with a detection limit of 10.7 μM. The proposed Dual-MFB was applied to measure Ca²⁺ concentration in cow blood, which provided a new method to diagnose hypocalcemia after dairy cow calving.     

CALMODULIN1 and WRKY53 Function in Plant Defense by Negatively Regulating the Jasmonic Acid Biosynthesis Pathway in Arabidopsis

Jasmonic acid (JA) is an important hormone that functions in plant defense. cam1 and wrky53 mutants were more resistant to Spodoptera littoralis than in the wild-type (WT) Arabidopsis group. In addition, JA concentration in cam1 and wrky53 mutants was higher compared with the WT group. To explore how these two proteins affect the resistance of Arabidopsis plants, we used a yeast two-hybrid assay, firefly luciferase complementation imaging assay and in vitro pull-down assay confirming that calmodulin 1 (CAM1) interacted with WRKY53. However, these two proteins separate when calcium concentration increases in Arabidopsis leaf cells. Then, electrophoretic mobility shift assay and luciferase activation assay were used to verify that WRKY53 could bind to lipoxygenases 3 (LOX3) and lipoxygenases 4 (LOX4) gene promoters and negatively regulate gene expression. This study reveals that CAM1 and WRKY53 negatively regulate plant resistance to herbivory by regulating the JA biosynthesis pathway via the dissociation of CAM1-WRKY53, then the released WRKY53 binds to the LOXs promoters to negatively regulate LOXs gene expression. This study reveals WRKY53′s mechanism in insect resistance, a new light on the function of WRKY53.     

集成膜工艺海水淡化与浓海水综合利用

综述了反渗透一电渗析集成工艺海水淡化与浓缩水综合利用研发现状与发展趋势。指出具有特殊分离功能的离子交换膜电渗析技术是高度分离、富集海洋化学元素的关键技术。提出了目前需要研究的几个问题。     

亚海水淡化技术处理滩涂水的中试

采用亚海水淡化技术对滩涂水库水淡化,建立了2m^3/h的试验工程,试验过程中对水温、吨水电耗、进水TDS、产水TDS等指标的相关性进行了研究。试验结果表明,滩涂水含盐量约为海水的1／10,在水温大于13℃时,吨水电耗低于2．5kWh,产水含盐量一般在200mg／L以内,出水水质达到国家生活饮用水卫生标准。     

神经元网络——它的背景、进展和问题

近年来神经元网络研究的进展,在国际学术界引起了强烈的轰动,成了各界学人的热门话题.本文分析了神经元网络研究的来龙去脉,简要介绍了若干代表性的进展,指出了这一研究的意义、问题和进一步研究的方向,希望引起各界同仁的关注.     

微机辅助设计广播天线阵

随着广播事业的迅速发展，中波广播的定向发射将很快在我国提上日程。由于天线阵综合的工作量和对经验的依赖都比较大，非专门工作者殊难进行。本文提出的借助微机辅助设计的方法，对天线阵有一般了解的人员即可用其设计广播天线阵。     

预应力砼井字梁结构测试分析

在预应力双向网格井字梁屋盖施工中，对井字梁的预应力摩擦损失、锚具变形、钢筋内缩、弹性压缩和反拱及挠度进行了实测，以检验梁中建立的有效预应力能否达到设计要求，并与理论值进行比较。