Multicolor Photo‐Crosslinkable AIEgens toward Compact Nanodots for Subcellular Imaging and STED Nanoscopy

Aggregation induced emission (AIE) has attracted considerable interest for the development of fluorescence probes. However, controlling the bioconjugation and cellular labeling of AIE dots is a challenging problem. Here, this study reports a general approach for preparing small and bioconjugated AIE dots for specific labeling of cellular targets. The strategy is based on the synthesis of oxetane‐substituted AIEgens to generate compact and ultrastable AIE dots via photo‐crosslinking. A small amount of polymer enriched with oxetane groups is cocondensed with most of the AIEgens to functionalize the nanodot surface for subsequent streptavidin bioconjugation. Due to their small sizes, good stability, and surface functionalization, the cell‐surface markers and subcellular structures are specifically labeled by the AIE dot bioconjugates. Remarkably, stimulated emission depletion imaging with AIE dots is achieved for the first time, and the spatial resolution is significantly enhanced to ≈95 nm. This study provides a general approach for small functional molecules for preparing small sized and ultrastable nanodots.     

Enhanced hydrothermal stability of ZSM-5 formed from nanocrystalline seeds for naphtha catalytic cracking

We successfully synthesized hydrothermally stable ZSM-5 with crystalline nano seeds. We employed a template-free method using ZSM-5 crystalline nano seeds and sodium silicate as a silica source. The prepared ZSM-5 exhibited uniform crystal size and relative crystallinity greater than 100 %. The size of the crystalline nano seed in the scale of 100 nm was found to be the optimum size for obtaining uniform, highly crystalline ZSM-5 with structural stability. After P-modification, the synthesized ZSM-5 with the optimally sized seed showed high hydrothermal stability and improved catalytic naphtha cracking activity compared to a commercial ZSM-5 catalyst. In order to find the elements for the increased hydrothermal stability, the samples were evaluated by studying crystallinity, aluminum spectrum, and acidity using XRD, solid-state NMR, and NH₃-TPD, respectively after steaming at 800 °C for 24 h. It is speculated that the increased hydrothermal stability of the ZSM-5 resulted mainly from the increased aluminum structural stability.     

The tetrahedral finite cell method: Higher‐order immersogeometric analysis on adaptive non‐boundary‐fitted meshes

The finite cell method (FCM) is an immersed domain finite element method that combines higher‐order non‐boundary‐fitted meshes, weak enforcement of Dirichlet boundary conditions, and adaptive quadrature based on recursive subdivision. Because of its ability to improve the geometric resolution of intersected elements, it can be characterized as an immersogeometric method. In this paper, we extend the FCM, so far only used with Cartesian hexahedral elements, to higher‐order non‐boundary‐fitted tetrahedral meshes, based on a reformulation of the octree‐based subdivision algorithm for tetrahedral elements. We show that the resulting TetFCM scheme is fully accurate in an immersogeometric sense, that is, the solution fields achieve optimal and exponential rates of convergence for h‐refinement and p‐refinement, if the immersed geometry is resolved with sufficient accuracy. TetFCM can leverage the natural ability of tetrahedral elements for local mesh refinement in three dimensions. Its suitability for problems with sharp gradients and highly localized features is illustrated by the immersogeometric phase‐field fracture analysis of a human femur bone. Copyright © 2016 John Wiley & Sons, Ltd.