Characterization of the brown pigment in sautéed onion by metal-chelating Sepharose 6B column chromatography and microbial decolorization

The brown pigment in sautéed onion was characterized by a microbiological procedure (involving microbial decolorization) and a chemical procedure (involving metal-chelating chromatography with a Sepharose 6B column). The suitability of the chemical procedure as a method for classifying brown pigments was also examined.The pigment from sautéed onion was about 40% decolorized by Coriolus versicolor IFO 30340, as were the model pigments caramel and model melanoidin (MM). The phenol-type model pigments (PTMP) were approximately 40-60% decolorized by Paecilomyces canadensis NC-1, but the sautéed-onion pigment, the MM and the caramel were merely 5% decolorized by it. The pigment from sautéed onion was separated into two components by Cu²⁺, Fe²⁺, or Zn²⁺-chelating chromatography, as were caramel and MM; the PTMP was not separated into multiple components by either Cu²⁺ or Fe²⁺-chelating chromatography. The results from both methods indicate that the pigment from sautéed onion is similar to MM and caramel but not the phenol-type pigment.     

Sex Attractant Pheromone from the Rice Stalk Stink Bug, Tibraca limbativentris Stal

The male-produced sex pheromone from the Brazilian rice stalk stink bug Tibraca limbativentris is reported. Olfactometer bioassays with sexually mature males and females showed that males attracted females, which suggests that males release a sex pheromone. Males were not attracted to either sex, nor were females attractive to conspecific females. Attraction of the females to males was highest at night. The headspace volatiles collected from male and female bugs were analyzed by gas chromatography (GC) and GC–mass spectrometry. Two male-specific compounds were identified as isomers of 1′S-zingiberenol, whereas a series of defensive compounds were identified in extracts from both sexes. Zingiberenol has three chiral centers, and the nonselective syntheses used produced two groups of isomers, zingiberenol I containing four isomers, namely (1RS,4RS,1′R)-4-(1′,5′-dimethylhex-4′-enyl)-1-methylcyclohex-2-en-1-ol, and zingiberenol II containing the other four isomers, namely (1RS,4RS,1′S)-4-(1′,5′-dimethylhex-4′-enyl)-1-methylcyclohex-2-en-1-ol. Both groups of stereoisomers were more attractive than hexane controls. The absolute configuration of the insect-produced pheromonal components remains to be elucidated, but the 1′S stereochemistry was established for at least one of the isomers.     

Quantitative risk assessment of noroviruses in drinking water based on qualitative data in Japan

Noroviruses are one of the major causes of viral gastroenteritis in Japan. A quantitative risk assessment was conducted to evaluate the health risk caused by this virus in drinking water. A Monte Carlo analysis was used to calculate both the probability of infection and the disease burden using disability-adjusted life years (DALYs). The concentration of noroviruses in tap water was estimated based on qualitative data and a most probable number (MPN) method with an assumed Poisson lognormal distribution. This numerical method was evaluated using two sets of available count data of Cryptosporidium: that collected from a river and that found in tap water in Japan. The dose-response relationships for noroviruses were estimated using assumed ID50 (10 or 100). The annual risk was higher than the US-EPA acceptable level (10(-4) [infection/ person-year]) but around the WHO level (10(-6) [DALYs/ person-year]). As suggested by others, since microbial concentrations are generally lognormally distributed, the arithmetic mean was directly related to the annual risk, suggesting that the arithmetic mean is more useful in representing the degree of microbial contamination than the geometric mean.     

On phenomenological failure criteria for composite bolted joint analysis

There has been a recent trend to use phenomenological strength models in combination with point stress or average stress failure criteria to form strength models for the analysis of composite bolted joints. Several papers published using these approaches have demonstrated practical reductions in the size of the empirical data base required to perform bolted joint analysis for specific materials and laminates. Since the inception of phenomenological failure models for bolted joints several refinements have been proposed, but the generality of the method is not clearly defined. This paper addresses the concepts underlying the formulation of these strength models, compares the capabilities of several commonly used failure criteria and discusses the limits of applicability through example analyses and comparison with experimental data. The results indicate that differences in predicted strengths for the models examined were insignificant. Failure mode predictions based on single point failure location information varied from model to model and differed significantly from experimentally observed failure modes for some geometries. The models cannot be applied with confidence for the analysis of a wide class of laminates and geometries without calibrating the model through empirical data.     

Lightweight Boolean Network Tomography Based on Partition of Managed Networks

Boolean network tomography is a promising technique to achieve fault management in networks where the existing IP-based troubleshooting mechanism cannot be used. Aiming to apply Boolean network tomography to fault management, a variety of heuristic methods for configuring monitoring trails and paths have been proposed to localize link failures in managed networks. However, these existing heuristic methods must be executed in a centralized server that administers the entire managed network and the methods present scalability problems when applied to large-scale managed networks. Thus, this paper proposes a novel scheme for achieving lightweight Boolean network tomography in a decentralized manner. The proposed scheme partitions the managed network into multiple management areas and localizes link failures independently within each area. This paper also proposes a heuristic network partition method with the aim of efficiently implementing the proposed scheme. The effectiveness of the proposed scheme is verified using typical fault management scenarios where all single-link failures and all dual-link failures are localized by the least number of monitoring paths on predetermined routes. Simulation results show that the proposed scheme can greatly reduce the computational load on the fault management server when Boolean network tomography is deployed in large-scale managed networks. Furthermore, the degradation of optimality in the proposed scheme can be mitigated in comparison with a centralized scheme that utilizes heuristics to reduce the computational load on the centralized server.     

Localization of the gene encoding the alpha subunit of human interleukin-5 receptor (IL5RA) to chromosome region 3p24-3p26

The chromosomal location of the human gene for the alpha subunit of interleukin-5 receptor (IL5RA) has been determined. The human IL5RA gene was localized to the short arm of chromosome 3 by Southern blot analysis of DNA from a panel of mouse-human hybrid somatic cell lines. The IL5RA gene has been further localized to human chromosome region 3p24-3p26 by in situ hybridization of a molecularly cloned IL5RA cDNA fragment to metaphase chromosomes. The results suggest that the IL5RA locus is unlinked to other members of the hematopoietic receptor family. Assignment of the IL5RA gene to chromosome 3 at bands p26-p24 raises the possibility that it may be altered by certain nonrandom chromosomal abnormalities arising in human hematopoietic malignancies and solid tumors.     

Sesamin is a potent and specific inhibitor of Δ5 desaturase in polyunsaturated fatty acid biosynthesis

Incubation with sesame oil increases the mycelial dihomo-γ-linolenic acid content of an arachidonic acid-producing fungus,Mortierella alpina, but decreases its arachidonic acid content [Shimizu, S., K. Akimoto, H. Kawashima, Y. Shinmen and H. Yamada (1989)J. Am. Oil Chem. Soc. 66, 237–241]. The factor causing these effects was isolated and identified to be (+)-sesamin. The results obtained in experiments with both a cell-free extract of the fungus and with rat liver microsomes demonstrated that (+)-sesamin specifically inhibits Δ5 desaturase at low concentrations, but does not inhibit Δ6, Δ9 and Δ12 desaturases. Kinetic analysis showed that (+)-sesamin is a noncompetitive inhibitor (K_i for rat liver Δ5 desaturase, 155 μM). (+)-Sesamolin, (+)-sesaminol and (+)-episesamin, also inhibited only Δ5 desaturases of the fungus and liver. These results demonstrate that (+)-sesamin and related lignan compounds present in sesame seeds or its oil are specific inhibitors of Δ5 desaturase in polyunsaturated fatty acid biosynthesis in both microorganisms and animals. On leave from Suntory Ltd.     

Efficiency of sodium hypochlorite, fumaric acid, and mild heat in killing native microflora and Escherichia coli O157:H7, Salmonella typhimurium DT104, and Staphylococcus aureus attached to fresh-cut lettuce

The effect of the disinfectant sodium hypochlorite (NaClO), with or without mild heat (50 degrees C) and fumaric acid, on native bacteria and the foodborne pathogens Staphylococcus aureus, Escherichia coli O157:H7, and Salmonella Typhimurium DT104 attached to iceberg lettuce leaves was examined. The retail lettuce examined consistently harbored 6 to 7 log CFU/g of native bacteria throughout the study period. Inner leaves supported 1 to 2 log CFU/g fewer bacteria than outer leaves. About 70% of the native bacterial flora was removed by washing five times with 0.85% NaCl. S. aureus, E. coli, and Salmonella allowed to attach to lettuce leaves for 5 min were more easily removed by washing than when allowed to attach for 1 h or 2 days, with more S. aureus being removed than E. coli or Salmonella Typhimurium. An increase of time for attachment of pathogens from 5 min to 2 days leads to decreased efficiency of the washing and sanitizing treatment. Treatment with fumaric acid (50 mM for 10 min at room temperature) was the most effective, although it caused browning of the lettuce, with up to a 2-log reduction observed. The combination of 200 ppm of sodium hypochlorite and mild heat treatment at 50 degrees C for 1 min reduced the pathogen populations by 94 to 98% (1.2- to 1.7-log reduction) without increasing browning.     

Effect of matrix metalloproteinase on autolysis of sea cucumber Stichopus japonicus

To investigate the relationship between matrix metalloproteinase (MMPs) and autolysis of sea cucumber Stichopus japonicus, the dermis homogenate was incubated at 25°C to induce autolysis. EDTA Na₂ and 1,10-phenanthroline were used to verify the effect of MMPs on autolysis, which was measured by soluble protein and protein pattern. Soluble protein level increased during a 6-h autolysis process. SDS-PAGE demonstrated obvious protein degradation with the concomitant occurrence of degradation products. The above two indicators could be inhibited significantly by EDTA Na₂ and 1,10-phenanthroline, indicating that MMPs might play a significant role in autolysis of sea cucumber.     

A three-segment anisomorphic constant life diagram for the fatigue of symmetric angle-ply carbon/epoxy laminates at room temperature

The anisomorphic constant fatigue life (CFL) diagram approach that was developed in an earlier study is further tested for applicability to the matrix-dominated fatigue failure in symmetric angle-ply carbon/epoxy laminates. An extension of the CFL diagram approach is also attempted to improve the accuracy of fatigue life prediction. The original anisomorphic CFL diagram approach can be used for approximately predicting the CFL diagrams for the [±30]_3S and [±45]_3S laminates, while it fails to accurately predict the CFL diagram for the [±60]_3S laminate due to its significant local distortion. For accommodating the anisomorphic CFL diagram approach to the local distortion in CFL curves due to a significant change in mean stress sensitivity in fatigue, a transitional segment is inserted between the tension–tension and compression–compression dominated segments. It is demonstrated that the three-segment anisomorphic CFL diagram approach allows improved predictions of the CFL diagrams and S–N relationships for the angle-ply laminates.