Long-term characteristics of XLPE cables

To study the long-term characteristics of XLPE cables installed in free air and in water, aging tests were conducted under various testing conditions using XLPE cables with both 3.5 mm and 6 mm insulation. From the Weibull plots of lifetime distribution under the voltage stress E_L as the minimum breakdown strength, the minimum value of time to breakdown t_L under the constant voltage was estimated. The results of accelerated aging tests of XLPE cables installed in free air demonstrated that the V-t characteristics of XLPE cables could not be described by the conventional inverse power law (t ∝ V⁻ⁿ) with a single constant life exponent n. Based on the microscopic observation of a sliced insulation removed from XLPE cables, it was concluded that bow-tie trees with longer tree length observed in cables tested in water were caused by the moisture from outside, whereas the trees in cables tested in free air were caused by the residual moisture originally existing in the insulation. The breakdown strength of the aged cables tested in water increases through cable drying. However, it does not recover to the original values.     

Comparative studies of in vitro and in vivo function of three different shaped bioartificial pancreases made of agarose hydrogel

Various kinds of bioartificial pancreas (BAP) have been developed in the past. There have been many disputes about the advantages and disadvantages of each BAP. However, little attention has been paid to the shape of the devices. In this study, three different shaped BAPs were made of the same material, agarose hydrogel. These are microbead-, rod- and disc-shaped BAPs, which are comparable to microcapsules, hollow fibre diffusion chambers and disc-shaped diffusion chambers, respectively, in shape. Their performances were studied by in vitro culture, mathematical simulations and in vivo implantation into diabetic animals. The islets in the BAPs could survive and release insulin for more than 100 d regardless of the BAP shape during in vitro culture. Numerical analyses showed that insulin release kinetics depended greatly on the thickness of the gel membrane but not on the shape of the agarose hydrogel. The microbead BAP normalized blood glucose levels of all five recipients for more than 100 d. Three of eight recipients of the rod BAP and only one of six recipients of the disc BAP demonstrated long normoglycaemia. These results suggest that the shape of the BAPs determines the in vivo functioning period of the BAPs and that the microbead is the most suitable shape for the BAP.     

Effects of ionizing radiation on proliferation and differentiation of osteoblast-like cells

Diagnostic radiation for immediate post-surgical assessment of osseointegrated dental implants has been discouraged, due to the possibility of detrimental effects of ionizing radiation on healing and remodeling of bone. To assess this possibility, we investigated the effects of ionizing radiation on proliferation and differentiation of osteoblasts using osteoblast-like cells isolated from the calvariae of newborn rats (ROB) and a clonal osteoblastic cell line (MC3T3-E1). The cells were exposed on day 3 to a single dose of x-rays at either 40, 100, 400, or 4000 mGy, respectively, from a linear accelerator radiotherapeutic machine (Linac) or a 40-mGy dose from a diagnostic chest x-ray machine. The effects of radiation on cell growth and alkaline-phosphatase-specific (ALP) activity were evaluated at three-day intervals after irradiation up to day 12 in ROB cells, and evaluated at day 12 in MC3T3-E1 cells. At the culture end-point, the effects on formation of bone-like nodules were also evaluated in both ROB and MC3T3-E1 cells. Exposure of 4000 mGy differentially affected the two cell types. It inhibited cell growth and alkaline phosphatase activity, and inhibited DNA content in MC3T3-E1 cells. This irradiation also strongly inhibited the formation of bone-like nodules in ROB cells. On the other hand, exposure of 40-, 100-, and 400-mGy (Linac) and 40-mGy (diagnostic quality) irradiation induced no significant changes in cell growth, alkaline phosphatase activity, and formation of bone-like nodules in ROB cells. These doses also induced no significant changes in DNA content and ALP activity in MC3T3-E1 cells. These results indicate that ionizing radiation at a single dose of up to 400 mGy induces no significant changes in cell growth and differentiation of osteoblast-like cells, at least in vitro. Higher radiation doses (4000 mGy) may exert different effects on cell proliferation and cell differentiation of osteoblasts, depending on the cell types affected. Thus, diagnostic radiation seems to have less effect on proliferation and differentiation of osteoblasts.     

Sustained release double-layered progesterone suppository for luteal support therapy

OBJECTIVES: To evaluate the risk of having a child with a congenital anomaly in relation to occupational exposure to low level ionising radiation in the pre-conception period. METHODS: A case-control study based on the Canadian congenital anomalies registry used record linkage techniques to identify congenital anomalies among male and female workers in Canada's largest electric company. Cases were defined as parents of a child with a congenital anomaly born between April 1979 and December 1986 who had a congenital anomaly diagnosed within the first year of life. Controls were an individually matched sample of parents of a liveborn child without an anomaly. Risk of congenital anomaly was assessed in relation to parental exposure to ionising radiation acquired through work within a nuclear generating station of an electric power company. Exposure was assessed according to employment, whether or not the worker was monitored for radiation exposure, and quantitative estimates of radiation dose. RESULTS: Employment within the electric power industry was not associated with an increased risk of congenital anomalies in the offspring of mothers or fathers. Risk estimates for workers monitored (those who are likely to be exposed to ionising radiation) were 1.75 (95% confidence interval (95% CI) 0.86 to 3.55) for mothers and 0.84 (95% CI 0.68 to 1.05) for fathers. Exposure for fathers before conception, defined cumulatively and for six months before conception, was not associated with increased risk of anomalies in their offspring. There were no significant increases in risk found between type of anomaly and any measure of exposure, although the statistical power in these groups was limited. The study had insufficient numbers to evaluate the effects of ionising radiation in mothers as only three mothers had recorded doses > 0 mSv. CONCLUSIONS: Overall, workers in a nuclear power industry, and specifically those exposed before conception to low levels of ionising radiation, do not appear to be at an increased risk of having a liveborn child with a congenital anomaly.     

Δ22-β-Muricholic acid in monoassociated rats and conventional ratsacid in monoassociated rats and conventional rats

Bile acids were analyzed in the bile, small and large intestines, and feces of germ-free rats after a single inoculation with one of six intestinal bacteria that had been originally isolated from human feces.Bacteroides vulgatus andBifidobacterium longum preferentially deconjugated tauro-β-muricholic acid and taurocholic acid, respectively.Clostridium ramosum, Peptostreptococcus productus andLactobacillus gasseri deconjugated both bile acids, butEscherichia coli did not deconjugate either one. Rats inoculated with bacteria that deconjugated tauro-β-muricholic acid produced Δ²²-β-Muricholic acid in the feces. In contrast, Δ²²-cholic acid could not be detected in rats inoculated with bacteria that deconjugated taurocholic acid.     

Grain-Boundary-Phase Identification of a Lead-Based Relaxor by X-ray Photoelectron Spectroscopy

X-ray photoelectron spectroscopy was used to study a lead-based relaxor dielectric ceramic to identify the grain-boundary phase most likely to be responsible for the insulation degradation of relaxor dielectric ceramics under humid loading conditions. The grain-boundary phase consisted mainly of lead and oxygen. The binding energies of Pb4f_7/2 and O1s in the grain-boundary phase were found to be 137.3 eV and 528.8 eV, respectively, and these values agreed well with those for Pb₃O₄ and PbO₂. In addition, the broadness of the Pb4f_7/2 peak suggested the presence of PbO. Measurements were made of lead dissolution from dielectric ceramics in hot water; specimens with a grain-boundary phase gave a 30 to 50 times greater lead concentration in the hot water than specimens without a grain-boundary phase. This demonstrated that the grain-boundary phase easily dissolved in hot water. Thus, it was concluded that the grain-boundary phase contained water-soluble PbO₂ and PbO. The results strongly supported the resistance degradation mechanism of relaxor dielectric ceramics under humid loading conditions as previously proposed by the authors.     

Effect of Hydrogen Fluoride Gas Treatment on High-Strength Fluoride-Fiber Fabrication

The effect of treating a ZrF₄-based fluoride-glass surface with hydrogen fluoride gas is investigated using secondary-ion mass spectroscopy. The analysis reveals that this treatment is effective in reducing the oxygen concentration at the surface. A strong fiber with a mean tensile strength of 550 MPa and maximum strength of 600 MPa is successfully fabricated by using this technique.     

The expression of invasive behavior of differentiated squamous carcinoma cell line evaluated by an in vitro invasion model

In order to elucidate the factors contributory to the expression of invasiveness of oral squamous cell carcinoma, we conducted biochemical and morphological comparisons of well differentiated squamous carcinoma cell line OSC-19 (oral squamous cell carcinoma) and undifferentiated carcinoma cell line KB, both cultured on 3T3 cell-embedded collagen gel (in vitro invasion model). OSC-19 cells invaded 3T3 cell-embedded collagen gel, while KB cells and OSC-19 cells on 3T3 cell-free gel matrix were less invasive. Cultured OSC-19 cells were characterized by lower proliferating activity, lower secretion of laminin and higher secretion of fibronectin than those of KB cells. Although the basement membrane with deposition of laminin and type IV collagen was formed, it was discontinuous at the invasion front. Gelatin zymography and western blotting showed matrix metalloproteinases (MMP), i.e., 72 kDa gelatinase (MMP-2) and 92 kDa gelatinase (MMP-9). Gelatinolytic activity was assayed, and was higher in OSC-19 cells than in KB cells or OSC-19 cells of the 3T3 cell-free model. By immunohistochemical analysis, MMP-2-positive cells were found scattered in both cell lines without any preferential localization, and the positivity for MMP-9 was localized in the invasion front of OSC-19 cells. These results strongly suggest that the invasiveness of squamous cell carcinoma is well correlated with cell-matrix adhesion by fibronectin and with focal elaboration of metalloproteinases, especially MMP-9, which play a major role in degrading the extracellular matrix components.     

CATALYTIC HYDROPYROLYSIS OF WESTERN CANADIAN COAL COMBINED WITH CRITICAL GAS EXTRACTION

Experimental results of catalytic hydropyrolysis combined with solvent extraction near critical conditions of an Alberta subbituminus coal indicated a conversion of 40 wt% of coal to hydrocarbon products. The effects of catalyst and solvent on the structural parameters of the extracts were evaluated from the modified Brown-Ladner's equation. Both alcoholic and aromatic solvents and Lewis acids (ZnCl₂ and SnCl₂ 2HO₂) showed Increased yields of hydrocarbon products, but the presence of hydrogen was necessary to improve this yield.