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61.
According to the investigations of Cameron and his coworkers1–5 into the chain-matching phenomena between normal paraffin as a solvent and straight-chain fatty acid as an additive, the optimum condition in boundary lubrication is attained when the chain lengths of the additive and solvent are equal. A series of experiments has been conducted over the past ten years on the role of the chain-matching effect, not only on boundary lubrication but also widely on relevant interfacial phenomena. The results of the investigations are discussed. 相似文献
62.
Sakai K Mori M Fujii A Iwami Y Chukeatirote E Shirai Y 《Journal of Bioscience and Bioengineering》2004,98(1):48-56
Reproducible amounts of lactic acid accumulate in minced kitchen refuse under open conditions with intermittent pH neutralization [Sakai et al., Food Sci. Technol. Res., 6, 140 (2000)]. Here, we showed that such pH-controlled open fermentation of kitchen refuse reproducibly resulted a selective proliferation of a major lactic acid bacterial (LAB) species. In one experiment, the predominant microorganisms isolated during the early phase (6 h) were Gammaproteobacteria. In contrast, those that predominated during the late phase (48 h) were always Lactobacillus plantarum in three independent experiments. To further quantify the microbial community within open lactic acid fermentation, we performed fluorescent in situ hybridization (FISH) analysis targeting 16S (23S) rRNA. We designed two new group-specific DNA probes: LAC722(L) was active for most LAB including the genera Lactobacillus, Pediococcus, Leuconostoc and Weisella, whereas Lplan477 was specific for L. plantarum and its related species. We then optimized sample preparation using lysozyme and hybridization conditions including temperature, as well as the formamide concentration and the salt concentration in the washing buffer. We succeeded in quantification of microorganisms in semi-solid, complex biological materials such as minced kitchen refuse by taking color microphotographs in modified RGB balance on pre-coated slides. FISH analysis of the fermentation of kitchen refuse indicated that control of the pH swing leads to domination by the LAB population in minced kitchen refuse under open conditions. We also confirmed that L. plantarum, which generates lactic acid in high quantities but with low optical activity, became the dominant microorganism in kitchen refuse during the late phase of open fermentation. 相似文献
63.
Kusano M Sakai Y Kato N Yoshimoto H Tamai Y 《Journal of Bioscience and Bioengineering》1999,87(5):690-692
Acetate ester synthesis was studied in vitro with the ethyl acetate-producing yeast Candida utilis. The level of enzyme activity observed for the NAD+-dependent hemiacetal dehydrogenase acting on hemiacetal, which was produced non-enzymatically from an alcohol and an aldehyde, was much greater than that for the other enzyme involved in ester synthesis, alcohol acetyltransferase. The level of ethyl acetate synthesis in vivo approximately paralleled the hemiacetal dehydrogenase (HADH) activity. The results suggest that the main pathway for ethyl acetate synthesis in C. utilis involves a novel hemiacetal dehydrogenase activity. 相似文献
64.
Direct modulation of an InGaAsP/InP double heterostructure laser was investigated experimentally. Sinusoidal modulation up to 2.5 GHz was achieved with almost constant modulation efficiency. Pulse responses showed that the damped relaxation oscillation in light output was well suppressed. 相似文献
65.
Room-temperature c.w. operation of InGaAsP/InP heterostructure lasers grown by liquid-phase epitaxy was achieved at 1.56 ?m. An active InGaAsP layer was essentially sandwiched by InP, though a thin InGaAsP buffer layer was deposited to prevent the melt-back of the active layer. Threshold current was typically 300 mA for a 17 ?m wide oxide-defined stripe laser. 相似文献
66.
The evolution mechanisms of new high-angle boundaries as well as ultrafine grains at large strains were studied by means of multidirectional forging (MDF) of pure copper at low temperature and aluminum alloy at high temperature, where dynamic recovery operates as a main restoration process. The structural changes can be characterized by the evolution of deformation bands such as microshear or kink bands at moderate strains. Multidirectional forging accelerates the evolution of many mutually crossing microshear or kink bands developed in various directions. The misorientations between (sub)grains increased gradually with increasing cumulative strain, finally leading to the development of a new fine-grained structure. The dynamic grain formation can be resulted from in situ or continuous dynamic recrystallization which is discussed in detail. 相似文献
67.
68.
Nakao Y. Itoh H. Hoshino S. Sakai Y. Tagashira H. 《Dielectrics and Electrical Insulation, IEEE Transactions on》1994,1(3):383-389
Prebreakdown phenomena in n-hexane are observed in detail for positive and negative polarities by using simultaneously a high speed schlieren technique and an LED current measuring system, when an impulse voltage (1.1/225 μs) is applied to a point-to-plane electrode gap. Furthermore, the effects of several additives on the streamer propagation are investigated. Especially the effects of electron-trapping additives on negative streamer propagation and of low ionization potential additives on the positive streamer propagation, are examined, as is a correlation between the shape and the propagation velocity of the streamers 相似文献
69.
Kimura T. Sakai Y. Tagashira H. Nakamura S. 《Dielectrics and Electrical Insulation, IEEE Transactions on》1994,1(4):644-647
Fast and slow electron current signals in N2/Ar liquid mixtures have been observed. From the fast signal, the drift velocity and life time of delocalized electrons in the liquid with N2 molar fractions X=N2/(N2+Ar) below 20 mol% were obtained for field strengths E between 5 and 45 kV/cm. The mobility for delocalized electrons in the liquids was shown to decrease exponentially with X as indicated in a previous paper. However, the mobility for localized electrons was shown to increase with increasing X, but only slightly around the N2 liquid value 相似文献
70.
Steroid cyclophanes, each having a macrocyclic ring attached to four bile acid moieties via chiral lysine connectors, were synthesized, and the binding of the 2-naphthylphenylketone (guest) to the steroid cyclophanes in water was investigated. The circular dichroism spectra of the steroid cyclophane with cholic acid and L-lysine were significantly affected by the binding of the guest, and the induced circular dichroism based on the absorption of the achiral guest was also observed. The binding of the guest to the steroid cyclophane with cholic acids and D-lysines induced changes in the circular dichroism spectra with the opposite sign of the molecular ellipticities. An induced circular dichroism spectral change was not observed upon binding of the guest to the analogous host without OH sites. These results strongly suggest that the guest is conformationally fixed through hydrogen bonding between the carbonyl group of the guest and the steroidal hydroxyl group of the host. The assembly of only four steroid residues on the macrocyclic ring probably provided a hydrophobic nanocavity for hydrogen bonding. 相似文献