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51.
We have developed a microdispenser array made of PDMS, in which a number of nanoliter-sized droplets can be accurately dispensed and mixed with the aid of specific channels under pneumatic pressure. In this system, hydrophobic and narrow channels act as a kind of valve and help structural liquid manipulation. Also, by arranging multiple dispensers in parallel, a single injection of liquid becomes sufficient for the preparation of multiple nanoliter-sized aliquots for different reactions. We designed two kinds of microdevices for multiple liquid dispensing and mixing and evaluated their performance and reproducibility, proving them sufficient for quantitative reactions. As a practical application, biochemical analysis of glucose was performed using enzymatic reactions. This liquid dispensing technology can be widely applied in the field of microscale analysis due to its low consumption, small dead volume of reagents and samples, and ease of operation. 相似文献
52.
Amizuka N Shimomura J Li M Seki Y Oda K Henderson JE Mizuno A Ozawa H Maeda T 《Journal of electron microscopy》2003,52(6):503-513
Previous studies have reported enhanced osteoclastogenesis, increased bone resorption and osteoporosis in osteoprotegerin (OPG)-deficient mice. In the present study, we show that the tibial epiphyses contain abundant, thin trabeculae lined with numerous osteoclasts and cuboidal osteoblasts. The increase in osteoblasts and osteoclasts was associated with a dramatic increase in calcein labelling of the mineralization fronts and replacement of much of the intertrabecular marrow with numerous alkaline phosphatase-positive preosteoblasts. Furthermore, the discrete, linear cement lines seen in wild-type mice were replaced by a randomly oriented meshwork of cement lines that were stained intensely for tartrate-resistant acid phosphatase and osteopontin in the OPG-/- mice. These indices of accelerated bone remodelling in mutant bone were associated with irregular trabecular surfaces, a disorganized collagen matrix interspersed with amorphous ground substance and numerous fissures between old and new bone. In total, these observations indicate that enhanced osteoclastic activity in OPG-/- epiphyses led to a coupled increase in osteoblast differentiation and activity and an increase in bone remodelling. The high bone turnover, disorganized matrix and impaired attachment of new to old bone in the cement lines in OPG-/- mice appear to cause bone fragility. 相似文献
53.
Shibatani S Fujiyama K Nishiguchi S Seki T Maekawa Y 《Journal of Bioscience and Bioengineering》2001,91(1):85-87
An active and soluble human beta1,4-galactosyltransferase (beta-GT) was produced in Escherichia coli using a maltose-binding protein fusion system. The purified recombinant beta-GT has a K(m) value of 0.035 mM for UDP-galactose and a V(max) of 643 x 10(3) nmol/mg/h. The enzyme catalyzes the transfer of galactose from UDP-galactose to N-linked oligosaccharides. The properties of the purified enzyme were identical to those of bovine milk beta-GT. 相似文献
54.
Fujiyama K Sakuradani S Moran DG Yoshida T Seki T 《Journal of Bioscience and Bioengineering》2001,91(4):419-421
We produced the human kidney Man9-mannosidase in Escherichia coli and studied the effect of the alpha-1,2-mannosidic linkage located in the alpha branch of Manalpha1-6(Manalpha1-2Manalpha1-3)Manalpha1-6(Manalpha1-3)Manbeta1-4GlcNAcbeta1-4-GlcNAc, an N-linked oligosaccharide, on the enzyme activity. The alpha1,2-mannose residue influenced the rate of hydrolysis of and substrate preference for other alpha-1,2-mannosidic linkages. 相似文献
55.
Motoyama Y. Murakami Y. Seki M. Kurauchi T. Kikuchi N. 《Electron Devices, IEEE Transactions on》2007,54(6):1308-1314
The sustain pulse voltage of the panel for 66-kPa Ne + Xe (5%-30%) is 20%-40% lower with a Sr0.62Ca0.38O protective layer than with a MgO protective layer. At a normal sustain voltage of 160-200 V, the luminous efficiency of the panel with the Sr0.62 Ca0.38O protective layer for Xe (30%) is about twice as high as with the MgO protective layer for Xe (10%). The luminances of these panels are almost the same. This high efficiency at normal sustain pulse voltage and normal luminance is obtained through the combined use of the Sr0.62Ca0.38O protective layer and high Xe content. With regard to ion bombardment, the Sr0.62Ca0.38O film has a 4.5 times longer life than SrO film and nearly 80% of the life of MgO film. We also calculated the values of theoretical secondary electron emission yield gammaimin of MgO, SrO, and CaO without energy bands in the band gap for rare gas ions and found that [ gammaimin of MgO] les [gammaimin of CaO] < [gammaimin of SrO] except for the one case with He. The breakdown voltage decreases with higher gammaimin values. As expected, the discharge voltage of the panel is much lower with the SrO protective layer than with the MgO protective layer. The discharge voltages of the panels with Sr0.62Ca0.38O and SrO protective layers are almost the same. These findings show that the life of the SrO protective layer can be made 4.5 times longer without any increase in the discharge voltage by adding CaO (40 at.%) 相似文献
56.
Yoshinobu Murakawa Motoaki Hara Hiroyuki Oguchi Yuichiro Hamate Hiroki Kuwano 《Microsystem Technologies》2013,19(8):1255-1259
This paper describes a new type hydrogen sulfide (H2S) gas sensor using ionic liquid (IL). In this sensor, a reservoir for the IL was integrated on a surface acoustic wave (SAW) resonator. The IL serves as an absorber for H2S gas. Mass change due to this absorption is detected as shift in the resonant frequency. In this study, we fabricated and demonstrated the sensor using the lithium niobate (LiNbO3) SAW resonator with the resonant frequency of 38 MHz. The integrated reservoir was filled by the IL 1-butyl-3-methylimidazolium hexafluorophosphate ([bmim]-[PF6]). As an experimental result, we observed the linear correlation between the frequency-shift and the exposure time of the sensor to the H2S gas. 相似文献
57.
Yoshihito Honsho Atsushi Asano Shu Seki Takeyoshi Sunagawa Akinori Saeki 《Synthetic Metals》2009,159(9-10):843-846
Intra-molecular mobility of positive charge carriers in poly(n-hexyl-[S]-2-methylbutylsilane) (PHMBS) films doped with N,N′-bis(2,5-di-tert-buthylphenyl)-3,4,9,10-perylenedicarboximide (PDI) is studied by time-resolved microwave conductivity (TRMC) measurements. PHMBS with optically active side chains has a rod-like tightly locked Si catenation with unlikely long persistence length compared with conventional dialkyl-substituted polysilanes. It was found that PDI is a suitable electron acceptor for PHMBS as it provides the high product of photo carrier generation yield ? as ~0.08% under an excitation at 355 nm. The efficient electron transfer reaction from PHMBS to PDI gives clear conductivity transients ascribed to the positive charges on the Si catenation of PHMBS by TRMC measurements. The estimated value of isotropic mobility along the tightly locked Si catenation is 0.36 cm2 V?1 s?1, suggesting the presence of mobile holes on the backbones of PHMBS. 相似文献
58.
Kaname Kanai Takahiro Miyazaki Takanori Wakita Kouki Akaike Takayoshi Yokoya Yukio Ouchi Kazuhiko Seki 《Advanced functional materials》2010,20(13):2046-2052
How annealing influences the morphology of a highly regioregular poly(3‐hexylthiophene) (RR‐P3HT) film at the substrate interface as well as the lateral inhomogeneity in the electronic structure of the film are elucidated. Whereas previous studies have reported that high‐molecular‐weight (MW) RR‐P3HT films tend to show low crystallinity even after annealing, it is found that high‐MW RR‐P3HT does show high crystallinity after annealing at high temperature for a long time. Photoemission electron microscopy (PEEM), X‐ray photoemission spectroscopy, and ultraviolet photoemission spectroscopy results clearly resolve a considerable lateral inhomogeneity in the morphology of RR‐P3HT film, which results in a variation of the electronic structure depending on the local crystallinity. The PEEM results show how annealing facilitates crystal growth in a high‐MW RR‐P3HT film. 相似文献
59.
Nakai M. Akui S. Seno K. Meguro T. Seki T. Kondo T. Hashiguchi A. Kawahara H. Kumano K. Shimura M. 《Solid-State Circuits, IEEE Journal of》2005,40(1):28-35
High-performance and low-power microprocessors are key to PDA applications. A dynamic voltage and frequency management (DVFM) scheme with leakage power compensation effect is introduced in a microprocessor with 128-bit wideband 64-Mb embedded DRAM. The DVFM scheme autonomously controls clock frequency from 8 to 123 MHz in steps of 0.5 MHz and also adaptively controls supply voltage from 0.9 to 1.6 V in steps of 5 mV, achieving 82% power reduction in personal information management scheduler application and 40% power reduction in MPEG4 movie playback. This low-power embedded microprocessor, fabricated with 0.18-/spl mu/m CMOS embedded DRAM technology, enables high-performance operations such as audio and video applications. As process technology shrinks, this adaptive leakage power compensation scheme will become more important in realizing high-performance and low-power mobile consumer applications. 相似文献
60.
In a digital library environment, we propose a novel support concept that is like a reference service in real libraries. Based on the concept, we have developed a prototype, called DOLPHIN, using a frame based knowledge base to support reference services in a digital library. DOLPHIN can guide the user to the appropriate service 相似文献