Sensitive Label-Free Immunoassay by Colorimetric Plasmonic Biosensors Using Silver Nanodome Arrays

The sensitive direct detection of biomolecules is demonstrated by a colorimetric plasmonic biosensor utilizing the surface colors of plasmonic metasurfaces named Ag nanodome arrays. The Ag nanodome arrays consist of polystyrene bead monolayers coated with Ag thin films whose surface colors are optimized by changing the size of the polystyrene beads. The bulk refractive index sensitivity of colorimetric detection evaluated using the hue angle is 590° RIU⁻¹ (RIU: refractive index unit). For selected geometry, the refractive index resolution (5.0 × 10⁻⁵ RIU) obtained by colorimetric detection surpasses that of spectroscopic detection evaluated via the dip wavelength in the reflection spectrum. The numerical simulations predict an enhanced sensing performance when the hue angle of the surface colors of the Ag nanodome arrays changes from 300° to 200°, corresponding to changes in the dip wavelength from 570 to 600 nm in the reflection spectra. Furthermore, the detection sensitivity of the biomolecules is characterized using a direct IgG immunoassay format. The detection limit of the IgG concentration is as low as 134 pM using simple colorimetric detection. The feasibility of sensitive label-free immunoassays using a colorimetric plasmonic biosensor is expected to accelerate the development of highly sensitive and reliable smartphone-based plasmonic biosensors.     

Broadband Tuning of a Continuous-Wave, Doubly Resonant, Lithium Triborate Optical Parametric Oscillator from 791 to 1620 nm

We report the use of extreme broadband, high reflectivity >99.5%, optical parametric oscillator (OPO) cavity mirrors. A continuous-wave, doubly resonant, OPO demonstrated tuning over a range of 791-1620 nm with a single mirror set. Wavelength tuning was performed by temperature tuning the nonlinear material of lithium triborate. Narrow linewidth oscillation was confirmed throughout the tuning range, and threshold pump power increased gradually from 50 mW near the degeneracy to 800 mW at the tuning band edge in a double-pass pumping configuration.     

Performance evaluation of immunity-based statistical en-route filtering in wireless sensor networks

Statistical en-route filtering (SEF) schemes can detect and eliminate false data injection attacks in wireless sensor networks. However, SEF does not address the identification of the compromised nodes which are injecting false reports. Therefore, we have proposed an immunity-based SEF to identify compromised nodes and achieve earlier detection of false reports. In the proposed scheme, each node has a list of neighborhood nodes and assigns credibility to each neighboring node. Each node can update the credibility of a neighboring node based on the success or failure of filtering and communication, and can then use the updated credibility as the probability of the next communication. In this article, some simulation results show that the immunity-based SEF outperforms the original SEF.     

Geometrical analysis and design of a motion transmitting element for mobile vehicles

The range of speed or torque of motors is limited to some degree. Industrial vehicles working in factories are required to output a large force when they carry loads, and also to move with high velocity when they move to their destination. However, it is impossible to realize both those requirements if a conventional reduction device is used. Velocity variation devices using gears are widely used to change the velocity ratio between the input and output shafts. However, the motion transmission from the input shaft to the output shaft is interrupted during the velocity ratio variation process. In order to solve this problem, a velocity variation method that can transmit motion continuously is proposed, in which a motion transmitting element is used. In this report, a motion transmitting element using the geometrical analysis method is proposed, a method of expressing the curve using dispersed points is proposed, and a method of calculating the inclination and the distances along the curve is developed. Based on the proposed methods, the geometrical form of the transmitting element is analyzed.     

New pixel driving circuit using self‐discharging compensation method for high‐resolution OLED micro displays on a silicon backplane

A new 4T2C pixel circuit formed on a silicon substrate is proposed to realize a high‐resolution 7.8‐μm pixel pitch AMOLED microdisplay. In order to achieve high luminance uniformity, the pixel circuit compensates its Vth variation of the MOSFET for the driving transistor internally by using self‐discharging method. Also presented are 0.5‐in Quad‐VGA and 1.25‐in wide Quad‐XGA microdisplays with the proposed pixel circuit.     

Slow eye movement as a possible predictor of reaction delays to auditory warning alarms in a drowsy state

In recently developed intelligent vehicles, warning alarms are often used to prompt avoidance behaviours from drivers facing imminent hazardous situations. However, when critical reaction delays to auditory stimulation are anticipated, the alarm should be activated earlier to compensate for such delays. It was found that reaction times to an auditory stimulus significantly increased in the presence of slow eye movement (SEM), which is known to occur frequently during the wake-sleep transition. The reaction delay could not be attributed to temporal effects such as fatigue and was invariant regardless of response effectors (finger or foot). Moreover, it was found that applied pedal force decreased immediately after an auditory stimulus interrupted SEM. Consequently, it was concluded that SEM can be a good predictor of reaction delays to auditory warning alarms when drivers are in a drowsy state. STATEMENT OF RELEVANCE: The present study demonstrated that simple auditory reaction time significantly increased when SEM emerged. In the design of vehicle safety systems using warning alarms to prompt avoidance behaviours from drivers, such reaction delays during SEM must be taken into account.     

Urban growth modeling of Kathmandu metropolitan region, Nepal

The complexity of urban system requires integrated tools and techniques to understand the spatial process of urban development and project the future scenarios. This research aims to simulate urban growth patterns in Kathmandu metropolitan region in Nepal. The region, surrounded by complex mountainous terrain, has very limited land resources for new developments. As similar to many cities of the developing world, it has been facing rapid population growth and daunting environmental problems. Three time series land use maps in a fine-scale (30 m resolution), derived from satellite remote sensing, for the last three decades of the 20th century were used to clarify the spatial process of urbanization. Based on the historical experiences of the land use transitions, we adopted weight of evidence method integrated in cellular automata framework for predicting the future spatial patterns of urban growth. We extrapolated urban development patterns to 2010 and 2020 under the current scenario across the metropolitan region. Depending on local characteristics and land cover transition rates, this model produced noticeable spatial pattern of changes in the region. Based on the extrapolated spatial patterns, the urban development in the Kathmandu valley will continue through both in-filling in existing urban areas and outward rapid expansion toward the east and south directions. Overall development will be greatly affected by the existing urban space, transportation network, and topographic complexity.     

薄壁曲线箱梁材料非线性分析的内力塑性系数法

目的 研究薄壁曲线箱梁考虑剪力滞效应和材料非线性的计算方法.方法 基于势能变分原理,推导了薄壁曲线箱梁的刚度方程.采用变刚度初法应力法,并结合截面内力塑性系数修正刚度系数,对薄壁曲线箱梁的材料非线性问题进行了求解.结果 通过算例计算,得到了曲线箱梁顶底板的应力分布以及挠度和扭转角的变化曲线.结论 笔者的计算结果与试验值整体吻合较好.对于薄壁曲线箱梁,悬臂板、顶板和底板,分别取三个不同的剪力滞翘曲位移函数的结果,精度优于三者取一个统一的剪力滞翘曲位移函数;随荷载的增加,弯曲剪力滞效应系数较挠度和扭转角更早进入非线性状态,翼缘截面上的应力分布逐渐趋于均匀,上翼缘应力的这种变化趋势要略滞后于下翼缘,而中支座截面翼缘应力的这种变化趋势则略快于左跨跨中和右跨跨中截面.     

基于6-DOF解耦操作臂的空间遥操作控制及运动学分析

针对由6-DOF解耦操作臂及其末端手爪组成的操作臂系统,设计了符合工程实际的遥操作控制策略,并提出了一种简单的运动学解算方法.采用Motoman工业操作臂,对提出的方法进行了实验验证.实验结果证明了所提方法的可行性.     

Effects of hematocrit variations on cerebral blood flow and oxygen transport in ischemic cerebrovascular disease

The contribution of hematocrit (Ht) changes on cerebral blood flow (CBF) and brain oxygenation in ischemic cerebrovascular disease is still controversial. In the present study, effects of Ht variations of CBF and oxygen delivery were investigated in patients with ischemic cerebrovascular disease. CBF was measured by the Xe-133 intracarotid injection method in 27 patients, whose diagnoses included completed stroke, reversible ischemic neurological deficit, and transient ischemic attack. Ht values in the patients ranged from 31 to 53%. There was a significant inverse correlation between CBF and Ht in these Ht ranges. Oxygen delivery, i.e., the product of arterial oxygen content and CBF, increased with Ht elevation and reached the maximum level in the Ht range of 40-45% and then declined. The CBF-Ht and oxygen transport-Ht relations observed in our study were similar to those in the glass-tube model studies by other workers rather than to those in intact animal experiments. From these results, it is conceivable that in ischemic cerebrovascular disease, the vasomotor adjustment was impaired in such a manner that the relations among Ht, CBF, and oxygen delivery were different from those in healthy subjects. Further, an "optimal hematocrit" for brain oxygenation was also discussed.