啤酒酵母吸附重金属离子铬的研究

将啤酒酵母自制成生物吸附剂，用于吸附重金属离子铬，考察了啤酒酵母吸附Cr^3＋过程中的影响因素，即pH值、初始Cr^3＋质量浓度、吸附时间、菌体浓度及酸碱预处理方法等因素。结果表 明，啤酒酵母对Cr^3＋的吸附量随pH值的增加而增大，吸附的最佳pH值范围是4～7，当pH7时达到吸附最大值；随着初始Cr^3＋质量浓度增加，吸附量有所提高，当溶液初始Cr^3＋质量浓度为80mg／L吸附效果最佳；最佳吸附时间为1h；菌体的最佳浓度为50mg／L；及啤酒酵母经碱处理后吸附量增大：正交试验确定反应时间为反应过程的显著因素，确定啤酒酵母对Cr^3＋的吸附过程遵循Langmuir方程。     

Characterization of petroleum-degrading bacteria from oil-contaminated sites in Vietnam

Four petroleum-degrading bacterial strains, 2TN-NB, 6TBX-CL, MVK2-5, and XCK, were isolated from various oil-contaminated sites in Vietnam. Determination of the nucleotide sequence of the gene encoding 16S rRNA allowed 2TN-NB to be identified as Acinetobacter sp. and the other three stains as Pseudomonas sp. Among the four isolates, 2TN-NB was most effective in degrading crude oil: in 1 d, it degraded 95% of the crude oil in the culture medium (5%, v/v). The isolated strains could also degrade a sulfur-containing aromatic hydrocarbon, dibenzothiophene (DBT), with low efficiency. Except for MVK2-5, which degraded crude oil least efficiently, the isolates produced biosurfactants in amounts sufficient for structural analysis. FT-IR measurement suggested that strains 6TBX-CL and XCK produced glycolipid-type biosurfactants while that produced by 2TN-NB was of the polysaccharide type.     

Speciation of selenium in selenium-enriched seeds,buckwheat (<Emphasis Type="Italic">Fagopyrum esculentum</Emphasis> Moench) and quinoa (<Emphasis Type="Italic">Chenopodium quinoa</Emphasis> Willdenow)

Buckwheat (Fagopyrum esculentum Moench) and quinoa (Chenopodium quinoa Willdenow) are widely used as food ingredients. The nutritional characteristics of these plants, i.e., high contents of proteins and amino acids suggest that selenium (Se) is preserved as selenoamino acid derivatives, in particular, selenomethionine (SeMet) in proteins, similar to selenized yeast. Therefore, buckwheat and quinoa are expected to be a good nutritional source of Se. Selenized buckwheat and quinoa were cultivated on Se-fortified soil using sparingly soluble Se salts, such as barium selenate and barium selenite. Se concentration in the edible parts of these plants was determined, and Se extraction efficiency with enzyme or alkali was evaluated. In addition, the chemical species of Se in the low molecular weight fraction of these plants were determined by HPLC-ICP-MS. Total Se concentrations in the edible parts of selenized buckwheat and quinoa were 170.4 ± 2.9 μg/g and 102.7 ± 2.4 μg/g wet weight, respectively. Thus, these selenized seeds were found to be Se accumulators. The results indicate that Se in selenized buckwheat exists mainly as SeMet, while Se in selenized quinoa exists not only as SeMet but also as selenate (Se(VI)) and non-protein forms.     

鳗鱼皮胶原蛋白水解物对结肠炎的改善作用

溃疡性结肠炎(UC)是一种特发性肠道疾病,发病率不断上升,且治疗药物都存在不同程度的副作用。研究发现胶原蛋白肽可缓解UC症状,并且安全、健康。然而,目前研究主要局限于UC的预防作用,缺少对其治疗作用的研究。鳗鱼皮富含胶原蛋白,而鳗鱼头一般在加工中被丢弃,造成资源浪费,目前缺少对鳗鱼皮胶原蛋白水解物的研究。本研究尝试利用鳗鱼头中的鱼皮提取胶原蛋白并进行水解,希望得到对UC具有治疗作用的鳗鱼皮胶原蛋白水解物(CH),提高鳗鱼加工副产物的利用价值。研究发现鳗鱼皮所含胶原蛋白丰富,占鱼皮湿重的27.87%,纯度较高且主要为Ⅰ型胶原蛋白,可作为胶原蛋白的优质来源。对胶原蛋白进行酶解,得到具有抗氧化活性的CH,并给药UC模型小鼠,结果显示:与阳性对照和模型组相比,CH可以安全健康地降低UC模型小鼠的DAI评分至0,并改善结肠状态;结肠组织病理切片表明CH可以恢复黏膜上皮完整性,减轻炎性细胞浸润;结肠组织氧化应激指标表明CH可以降低结肠组织MDA含量(0.75 nmol/mg pro)和MPO活性(0.11 U/g tissue),并通过提高GSH含量(22.92 umol/g pro)和SOD(693.61 U/g pro)、CAT(1.69 U/g pro)、GPx(141.29 U/g pro)3种抗氧化酶活性来提高结肠组织抗氧化能力,降低氧化应激水平,从而治疗UC。     

应对脱离配电网情况的单三相多微网组合分析及切换控制

考虑单相及三相微网两者在地域上接入较为相近,互联而形成单三相多微网系统。由于其复杂的运行方式,亟待解决如何实现多微网灵活无缝切换的问题。介绍了单三相多微网系统拓扑结构;当多微网系统为应对脱离配电网情况时,即常规主动并转离,通过互联方案预筛选得到初始方案后,基于模糊多目标决策得到多微网互联方案;通过给定的互联方案目标进行系统状态平滑切换。所提策略经算例验证可行,为多微网切换控制技术提供建议性方案。     

一种直流微电网多光伏变换器新型功率分配策略

为了解决光伏单元在运行过程中因工作模式切换造成的母线电压波动问题,提出一种新型平滑切换控制策略。根据光伏电池的输出特性曲线,将输出功率对输出电流的微分作为控制变量。通过跟踪不同的dp/di指令值来实现光伏单元最大功率点跟踪模式、恒压下垂模式的控制以及两种模式间的平滑切换。在此基础上,针对多光伏变换器工作在恒压下垂控制时因线路阻抗差异造成功率分配精度较低的问题,提出一种基于二次调节的自适应下垂控制策略。此外,构建相邻光伏单元间的稀疏通信网络,并采用动态一致性算法实现相关平均信息的全局稳定收敛。仿真和实验结果表明,所提控制策略可以实现光伏单元工作模式切换过程平滑过渡和负荷功率的精确分配。     

基于证据权法的循化县积石镇地质灾害孕灾分区及孕灾因素简析

积石镇位于青海省循化县北部,黄河沿岸,是循化县的政治、经济和文化中心。由于镇内地形破碎、沟壑纵横,地质环境条件差,是泥石流、滑坡、崩塌等地质灾害的高发区。随着境内基础设施建设的快速发展,人类工程经济活动对地质环境的破坏日益加剧,地质灾害的潜在危害也愈发严重。前人研究主要集中在该区域地质灾害体的基本特征,对于区域地质灾害的孕灾因子和规律尚未进行详细分析。本文通过证据权法,结合野外调查选取了研究区的孕灾因子,并在ArcGIS软件平台的支持下,对研究区的孕灾地质条件进行了分区。通关研究分析区域内的高程、坡度、坡向、地形起伏度、距断层距离、距河流距离、距公路距离、工程地质岩组共8个孕灾因子,进而总结出研究区的地质灾害孕灾规律。本研究可有效预防和减轻地质灾害对人民生命财产的危害,为积石镇的城镇规划和合理开发利用提供重要的基础依据。     

A rapid-detection method for radioactive cesium in the air

We propose a method of rapidly detecting radioactive cesium floating in the air for the purpose of alerting people active in the ‘difficult-to-return zone’ of Fukushima to changes in airborne radioactivity. With this purpose in mind, we set requirement specifications and created a new system concept. The targeted detection limit was 30 Bq/m³ and the mandatory level of measurement time was 48 min with a target level of 12 min, and these periods are 10 times faster than those of conventional air monitors. The system consists of a conventional gamma-spectrometer with a novel shaped shield. The targeted peak region of the measured energy spectrum is analyzed. After the basic design and confirmation of feasibility, we perform some experiments in the normal environment of Yokohama city and the difficult-to-return zone of Fukushima. Using the results, we conducted the design improvement and performance evaluation. As a result, the system performance is found to satisfy requirements. We thus conclude that this system is a promising candidate for rapid detection of airborne radioactivity in the difficult-to-return zone of Fukushima.