Hydroxypropyl-Distarch Phosphate from Potato Starch Increases Fecal Output, but Does Not Reduce Zinc, Iron, Calcium, and Magnesium Absorption in Rats

The physiological effects of hydroxypropyl‐distarch phosphate (HDP) were examined in rats. Male rats were fed a fiber‐free purified diet containing gelatinized potato starch (PS, 50 g/kg diet) that was not modified chemically or gelatinized, chemically modified potato starch (CMS, 50 g/kg diet) for 21 d. PS was used as the control. Six kinds of gelatinized HDP from potato with 2 different degrees of hydroxypropylation and 3 different degrees of cross‐linking were used as CMS. The wet weight and moisture of the fecal output of the rats fed highly hydroxypropylated HDP was 70% and 30% greater, respectively, than that in the control rats. The weights of the cecal wall and content of the rats fed highly hydroxypropylated HDP were 15% and 30% higher, respectively, than those of the control rats. The apparent absorption of Mg was 15% higher in the rats fed highly hydroxypropylated HDP than in the control rats. On the other hand, the apparent absorptions of Ca, Zn, and Fe were not affected by the diet. Cross‐linking did not influence the above‐mentioned physiological effects of HDP. Fecal excretion of bile acids and the plasma cholesterol concentration were not affected by the diet. These results show that the physiological effects of HDP depend on the hydroxypropylation but not the cross‐linking.     

Design,Synthesis, and Biological Evaluation of Lysine Demethylase 5 C Degraders

Lysine demethylase 5 C (KDM5C) controls epigenetic gene expression and is attracting great interest in the field of chemical epigenetics. KDM5C has emerged as a therapeutic target for anti-prostate cancer agents, and recently we identified triazole 1 as an inhibitor of KDM5C. Compound 1 exhibited highly potent KDM5C-inhibitory activity in in vitro enzyme assays, but did not show strong anticancer effects. Therefore, a different approach is needed for the development of anticancer agents targeting KDM5C. Here, we attempted to identify KDM5C degraders by focusing on a protein-knockdown strategy. Compound 3 b , which was designed based on compound 1 , degraded KDM5C and inhibited the growth of prostate cancer PC-3 cells more strongly than compound 1 . These findings suggest that KDM5C degraders are more effective as anticancer agents than compounds that only inhibit the catalytic activity of KDM5C.     

Upregulated Expression of Activation-Induced Cytidine Deaminase in Ocular Adnexal Marginal Zone Lymphoma with IgG4-Positive Cells

Immunoglobulin G4-related disease (IgG4-RD) is a systemic disorder characterized by tissue fibrosis and intense lymphoplasmacytic infiltration, causing progressive organ dysfunction. Activation-induced cytidine deaminase (AID), a deaminase normally expressed in activated B-cells in germinal centers, edits ribonucleotides to induce somatic hypermutation and class switching of immunoglobulin. While AID expression is strictly controlled under physiological conditions, chronic inflammation has been noted to induce its upregulation to propel oncogenesis. We examined AID expression in IgG4-related ophthalmic disease (IgG4-ROD; n = 16), marginal zone lymphoma with IgG4-positive cells (IgG4+ MZL; n = 11), and marginal zone lymphoma without IgG4-positive cells (IgG4- MZL; n = 12) of ocular adnexa using immunohistochemical staining. Immunohistochemistry revealed significantly higher AID-intensity index in IgG4-ROD and IgG4+ MZL than IgG4- MZL (p < 0.001 and = 0.001, respectively). The present results suggest that IgG4-RD has several specific causes of AID up-regulation in addition to inflammation, and AID may be a driver of oncogenesis in IgG4-ROD to IgG4+ MZL.     

Multiresidue analysis of pesticides in agricultural products by GC/MS coupled with database software

We evaluated a multiresidue method for determination of pesticides in agricultural products by SCAN mode GC/MS coupled with three kinds of database for 253 pesticides: relative retention time, mass spectra and calibration curve (SCAN method). Twenty-six pesticides, a total of 131 pesticides were detected in samples by the SCAN method. The detection results agreed closely with those of the SIM mode GC/MS method using calibration standards (SIM method). The ratios of the SCAN method to the SIM method ranged from 0.3 to 3.1 with SD values of 0.63. It was judged that the SCAN method could be applied to the screening analysis of pesticide residues in agricultural products, provided that the sample preparation method makes it possible to effectively remove sample matrixes with minimal loss of analytes.     

Characterization of endogenous enzymes of milled rice and its application to rice cooking

The relationship between the activities of endogenous enzymes in milled rice and accumulation of chemical components in the rice grains during cooking was investigated. Maltose and soluble starch were optimally hydrolyzed around 60 °C by both crude extracts and purified α-glucosidases of milled rice. Gelatinization onset temperature of rice flour was determined to be 63 °C with DSC analysis under usual cooking condition. Hydrolytic activities on carbohydrates and proteolytic activities of milled rice were enhanced at pH 5 compared to pH 7 at the range of 4–80 °C. When rice was cooked at pH 5, glucose and amino acids highly accumulated with soaking for 16 h before heating. We propose a new method of cooking with prolonged soaking at acidic pH, which is effective for increasing the amounts of chemical components in cooked rice.     

Accumulation of Arsenic and Copper by Bryophytes Growing in an Aquatic Environment near Copper Mine Tailings

Bryophytes with high As accumulation affinity were identified in the aquatic environment. We surveyed a stream near copper mine tailings and then conducted laboratory experiments to confirm As accumulation in the bryophytes with high As affinity. We found that a moss, Scopelophila cataractae, accumulates As in addition to Cu in aquatic environments and confirmed it in laboratory experiments. The highest value for As in S. cataractae from the field survey was 1300 mg/kg dry weight at relatively low As concentrations in the stream water (0.005 mg/L). In addition, Brachythecium plumosum and Rhynchostegium riparioides may also be useful bryophytes for accumulation of Cu and As, though the mechanisms of As accumulation might differ between these two bryophytes and S. cataractae.     

Metals in recycled polyethylene terephthalate and discrimination method for its use

Metals in recycled polyethylene terephthalate (PET) were analyzed by ICP-MS following microwave digestion with nitric acid. Physically and superclean-like recycled PET contained both Ge and Sb, and sometimes contained Co, P or Si. In contrast, the chemically recycled PET contained only Ge or Sb, and some samples contained Co. The recycled PETs did not contain Pb or Cd. Ge and Sb were catalysts of the polymerization, and the other metals also originated from the PET resin as additives. It was concluded that there is no safety concern about metals in recycled PET. It became clear that the presence of both Ge and Sb could identify products formed using physically or superclean-like recycled PET. According to this discrimination method, about half of the sheet molding products used recycled PET.     

Plasma LCAT Activity and Lipid Subfraction Composition in Obese Beagles Undergoing Weight Loss

The relationship between lecithin:cholesterol acyltransferase (LCAT) activity and weight loss in dogs was investigated. Four experimental weight-loss diets were fed to 12 obese female beagles for 56 days in a partial crossover design (n = 6). High- (HGI) or low- (LGI) glycemic index starch and diacylglycerol or triacylglycerol oils were combined to compose experimental diets with similar fatty acid profiles. Food intake and body weights were measured daily and weekly, respectively. Fasted blood samples were drawn at day 0, day 28, and day 56 to measure plasma LCAT activity and total (TC), unesterified (UC), and esterified (EC) cholesterol concentrations, and for fatty acid analysis of the phospholipid (PL) and EC fractions. The LGI groups lost more weight than the HGI groups due to starch digestibility differences. An HGI starch effect on TC and UC concentrations was observed but was unrelated to weight loss. LCAT activities increased over time but were not different after controlling for percentage weight loss. However, a positive linear correlation was found between LCAT and UC concentrations in all groups. Plasma PL fatty acid profiles reflected the diets fed, but increases in 16 and 18 carbon saturated and monounsaturated fatty acids in all groups appeared to be an effect of fatty acid mobilization from storage sites. Both plasma PL and EC fatty acid profiles were similar with both acylglycerol types and EC fatty acids reflected linoleic acid specificity with minimal diet or time effects.     

Split Enzyme-Based Biosensors for Structural Characterization of Soluble and Insoluble β-Glucans

β-Glucan is widely distributed in various plants and microorganisms and is composed of β-1,3-linked d-glucose units. It may have a branched short or long side chain of glucose units with β-1,6- or β-1,4-linkage. Numerous studies have investigated different β-glucans and revealed their bioactivities. To understand the structure-function relationship of β-glucan, we constructed a split-luciferase complementation assay for the structural analysis of long-chain β-1,6-branched β-1,3-glucan. The N- and C-terminal fragments of luciferase from deep-sea shrimp were fused to insect-derived β-1,3-glucan recognition protein and fungal endo-β-1,6-glucanase (Neg1)-derived β-1,6-glucan recognition protein, respectively. In this approach, two β-glucan recognition proteins bound to β-glucan molecules come into close proximity, resulting in the assembly of the full-length reporter enzyme and induction of transient luciferase activity, indicative of the structure of β-glucan. To test the applicability of this assay, β-glucan and two β-glucan recognition proteins were mixed, resulting in an increase in the luminescence intensity in a β-1,3-glucan with a long polymer of β-1,6-glucan in a dose-dependent manner. This simple test also allows the monitoring of real-time changes in the side chain structure and serves as a convenient method to distinguish between β-1,3-glucan and long-chain β-1,6-branched β-1,3-glucan in various soluble and insoluble β-glucans.