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排序方式: 共有369条查询结果,搜索用时 15 毫秒
31.
We described previously (H. Imamura, et al., Cancer Res., 54: 3620-3624, 1994) a quantitative and reproducible 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay for tumor cell invasiveness that uses a water-repellent, paraffin-treated Chemotaxicell chamber to produce a uniform Matrigel layer. In the present experiments, we studied 71 human gastrointestinal carcinomas, including 53 maintained as xenografts in nude mice and 18 fresh surgical specimens. We found a correlation between metastatic behavior and the percent invasion (PI) calculated from the MTT assay. Tumors producing liver metastases had a significantly higher PI than did tumors without liver metastases (P < 0.01), and seven of nine fresh tumors with a PI greater than 1.0 showed liver metastases within 2 years. No significant correlations were noted between the PI and clinicopathological factors. In the tumor xenografts, type IV collagenase activity was significantly higher in tumors with clinically evident liver metastases than in those without liver metastases (P < 0.05). Colorectal carcinomas with liver metastases and a high PI showed higher expression of matrix metalloproteinase 9 than matrix metalloproteinase 2 as assessed by gelatin zymography. Thus, the invasion-MTT assay is clinically useful for predicting liver metastases. Type IV collagenase plays an important role in the development of liver metastases from human gastrointestinal carcinoma. 相似文献
32.
We hypothesize that the interaction between protein kinase C (PKC) and nitric oxide (NO) plays a role in the modulation of cerebral vascular tone, and the disturbance of this interaction following subarachnoid hemorrhage (SAH) results in vasospasm. To prove this hypothesis with direct evidence, PKC activities of smooth muscle cells of canine basilar arteries in the control and in the SAH groups were measured by an enzyme immunoassay method. N omega-nitro-L arginine (L-NA), an inhibitor of NO production, enhanced PKC activity. This enhancement was inhibited neither by 8-bromo-guanosine 3',5'-cyclic monophosphate (8-bromo-cGMP) nor SIN-1, a NO releasing agent. PKC activity in the SAH was significantly higher than in the control; however, no further enhancement was produced with L-NA. In the SAH, PKC activity was not inhibited either by 8-bromo-cGMP or SIN-1. We conclude that NO maintains an appropriate vascular tone through inactivation of PKC, and that this effect is disturbed following SAH, resulting in PKC-dependent vascular contraction, such as vasospasm. On the other hand, once PKC has been activated, NO precursors do not inhibit PKC. These facts indicate NO inactivates PKC through the inhibition of phosphatidylinositol breakdown. 相似文献
33.
K Uemura H Otani K Sakamoto Y Sakanishi T Ota T Kobayashi N Ariyoshi A Shirahata 《Canadian Metallurgical Quarterly》1995,43(11):1173-1177
To our knowledge, this report represents the first confirmed case in Japan of a 15-year-old boy with acute lymphoblastic leukemia (ALL).L3 with hand-mirror cells (HMC) in the bone marrow. HMC lymphoid leukemia is an unusual variant of ALL in which the bone marrow lymphoblasts manifest distinctive hand-mirror morphologic features. HMC lymphoblast is characterized by an asymmetric foot-like cytoplasmic process that extends from the portion of the cell, thus giving it the light-microscopic appearance responsible for its name. Besides ALL, HMC has been reported in acute myeloblastic leukemia (AML), blastic crisis of chronic myelogenous leukemia, non-Hodgkin's lymphoma, and infectious mononucleosis. HMC has been reported to be prevalent in ALL.L1 and L2 as compared with L3. 相似文献
34.
Manabu Iguchi Tsuneo Kondoh Zen-Ichiro Morita Keiji Nakajima Kazuharu Hanazaki Tomomasa Uemura Fujio Yamamoto 《Metallurgical and Materials Transactions B》1995,26(2):241-247
Laser Doppler velocimeter (LDV) measurements were made to clarify the fluid flow behavior in a bath subject to centric bottom
gas injection. Correlations of the axial mean velocity and turbulence components in the gas-liquid two-phase flow region,i.e., in the bubbling jet region, were proposed as functions of the inner diameter of nozzle, gas flow rate, and densities of
gas and liquid. Measured values of the flow rate, momentum, and kinetic energy of water rising upward were approximated satisfactorily
by these empirical correlations. In addition, the Reynolds shear stress was calculated and compared with measured values.
Formerly Graduate Student, Osaka University, 相似文献
35.
We present the results of an experimental and analytical study of two-dimensional normal zone propagation in pancake test coils, wound with silver-sheathed BSCCO-2223 tapes. Two test coils were studied in detail, one having three and the other eight layers. Each test coil was housed in an adiabatic environment whose temperature (20-70 K) was controlled and maintained by a two-stage G-M cryocooler and placed in a background field (0-6 T) generated by a Bitter magnet. With a test coil carrying a transport current (0-200 A), a local heat disturbance was applied by a heater attached to the outermost layer of the coil. The resulting electrical and thermal responses of the coil were recorded with voltage taps and thermometers attached to the coil. A normal zone propagation code was developed to accurately simulate the voltage and temperature responses of each coil for both quenching and recovering events. The code solves the nonlinear transient heat diffusion equation in two-dimensional cylindrical coordinates with a finite difference method. As an application of this code, a two-coil system, with each coil comprised of one double pancake wound with silver-sheathed BSCCO tape, was studied for its quench behaviour as one of the coils was driven normal locally. The simulation results indicate that the value of a shunt resistor connected across the terminals of each coil has a profound effect on the level of hot-spot temperature reached in the quench initiation spot. 相似文献
36.
37.
Yoshimitsu Uemura Takami Kai Rintarou Natori Takeshige Takahashi Yasuo Hatate Masahiro Yoshida 《Renewable Energy》2004,29(4):581
A study was carried out to see if the potential of renewable energy sources other than hy droelectric power, such as wind, photovoltaic, solar thermal, biomass and waste energy sources, can meet the current energy consumption in Yakushima. The current electricity consumption can be covered by wind and photovoltaic energy sources. The total potential of wind and photovoltaic energy sources is 5.4 times as much as the current electricity consumption. LP gas and kerosene can be replaced by solar thermal and biogas energy. The potential of plant biomass and municipal waste is not sufficient (approximately one third) to cover the rest of the fossil fuels (gasoline, diesel oil and heavy oil). Also, plant biomass and municipal waste must be converted into fluid form. This shortage can be covered by the po tential of wind and photovoltaic energy sources. We also investigated the possibility of tourism expansion using the potential of wind and photovoltaic energy sources. Taking into account three types of capacity (energy, accommodation and transportation), Yakushima can accept approximately four times as many tourists as the current number of tourists. 相似文献
38.
Thermocapillary convection in a half‐zone liquid bridge of high Prandtl number fluid is widely known to exhibit a three‐dimensional oscillatory flow after the onset of oscillation. The oscillatory flow presents ‘standing’ and ‘traveling’ flows depending upon the temperature difference between the top and bottom rods. In the oscillatory state, the flow shows a modal structure with an azimuthal wave number that depends on the aspect ratio of the liquid bridge and the intensity of the thermocapillarity expressed by the Marangoni number. The present study attempted to control the azimuthal wave number by heating the free surface locally with a prescribed frequency and intensity. The flow in the liquid bridge exhibited different modal structures depending on the heating conditions and a relationship between the frequency and the modal structure was obtained. © 2005 Wiley Periodicals, Inc. Heat Trans Asian Res, 34(7): 460–469, 2005; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/htj.20086 相似文献
39.
Kentaro Kuratani Masayasu Uemura Minoru Mizuhata Akihiko Kajinami Shigehito Deki 《Journal of the American Ceramic Society》2005,88(10):2923-2927
We report on the direct deposition of high-quality ZrO2 thin films on various kinds of substrates by the liquid phase deposition method. After reaction for 24 h, thin films formed on various kinds of substrates, and the obtained thin film was comprised of densely packed nano-sized particles. The film annealed at 500°C showed a tetragonal phase at room temperature and this phenomenon has been discussed from the viewpoint of crystallite size effect. The result of optical transmittance measurement revealed that high transparency, more than 70% transmittance, has been achieved for the film after annealing at 900°C. 相似文献
40.
Daisuke Hiramatsu Kaoru Koyanagi Kaiichirou Hirayama Yoichi Uemura Mikio Kakiuchi Toshiyuki Satoh Akahiro Ara 《Electrical Engineering in Japan》2005,153(3):31-40
This paper describes the effect of mutual leakage reactance in the rotor circuit on the analysis of generator transient characteristics. The authors derive a detailed equivalent circuit model for a 48P‐15,750‐kVA generator, analyze sudden short circuits and asynchronization phenomena of the generator by simulations with an electromagnetic transients program (ATP‐EMTP), and compare the results with experimental data. © 2005 Wiley Periodicals, Inc. Electr Eng Jpn, 153(3): 31–40, 2005; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/eej.20147 相似文献