全文获取类型
收费全文 | 294篇 |
免费 | 19篇 |
国内免费 | 1篇 |
专业分类
电工技术 | 10篇 |
化学工业 | 101篇 |
机械仪表 | 7篇 |
建筑科学 | 6篇 |
能源动力 | 7篇 |
轻工业 | 77篇 |
水利工程 | 1篇 |
无线电 | 20篇 |
一般工业技术 | 34篇 |
冶金工业 | 18篇 |
原子能技术 | 2篇 |
自动化技术 | 31篇 |
出版年
2024年 | 1篇 |
2023年 | 1篇 |
2022年 | 10篇 |
2021年 | 11篇 |
2020年 | 1篇 |
2019年 | 3篇 |
2018年 | 10篇 |
2017年 | 5篇 |
2016年 | 9篇 |
2015年 | 5篇 |
2014年 | 14篇 |
2013年 | 20篇 |
2012年 | 11篇 |
2011年 | 21篇 |
2010年 | 18篇 |
2009年 | 13篇 |
2008年 | 15篇 |
2007年 | 12篇 |
2006年 | 18篇 |
2005年 | 14篇 |
2004年 | 9篇 |
2003年 | 11篇 |
2002年 | 15篇 |
2001年 | 9篇 |
2000年 | 3篇 |
1999年 | 7篇 |
1998年 | 4篇 |
1997年 | 4篇 |
1996年 | 8篇 |
1995年 | 3篇 |
1993年 | 3篇 |
1992年 | 5篇 |
1991年 | 3篇 |
1990年 | 5篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1982年 | 2篇 |
1981年 | 2篇 |
1973年 | 2篇 |
1971年 | 1篇 |
1968年 | 1篇 |
1966年 | 1篇 |
排序方式: 共有314条查询结果,搜索用时 31 毫秒
21.
Hung-Hsuan Huang Aleksandra Cerekovic Igor S. Pandzic Yukiko Nakano Toyoaki Nishida 《AI & Society》2009,24(3):225-235
Embodied conversational agents (ECAs) are computer-generated, human-like characters that interact with human users in face-to-face
conversations. ECA is a powerful tool for representing cultural differences and is suitable for interactive training or edutainment
systems. This article presents preliminary results from the development of a culture-adaptive virtual tour guide agent for
serving Japanese, Croatian, and general Western users by displaying appropriate verbal and non-verbal behaviors. It is being
implemented in Generic ECA Framework, a modular framework for developing ECAs. Dividing the ECA functions into reusable and
loosely coupled modules minimizes the effort required to implement additional behavior and facilitates incremental scale up
of the system. 相似文献
22.
23.
Sone T Nagamori E Ikeuchi T Mizukami A Takakura Y Kajiyama S Fukusaki E Harashima S Kobayashi A Fukui K 《Journal of Bioscience and Bioengineering》2002,94(1):87-91
We have produced micrometer-sized calcium alginate beads referred to as "bio-beads" that encapsulate plasmid DNA molecules carrying a reporter gene. In order to evaluate the efficiency of the bio-beads in mediating genetic transfection, protoplasts isolated from cultured tobacco cells (BY-2) were transfected with bio-beads containing a plasmid that carries the modified green fluorescent protein gene CaMV35S-sGFP. With the bio-beads treatment, approximately ten-fold higher GFP expression was observed after 24 h incubation compared to that with the conventional method using a naked plasmid solution. Transfection was up to 0.22% efficient. These results indicate that bio-beads have a possibility for efficient transformation in plants. 相似文献
24.
25.
The low-temperature grafting of butadiene on polyethylene (PE) preirradiated in air is compared with that on PE preirradiated in vacuo for the effect of several factors on the grafting. Little fundamental difference between the two cases was observed. In both cases the graft conversion curve is trapped radical type and the overall activation energies are 9.7 and 8.4 kcal/mol for 0.947-density PE and 0.916-density PE, respectively. Percent grafting is proportional to the square root of the dose rate (= total dose). The retarding effect of oxygen at irradiation or storage after irradiation on the grafting scarcely appears when the irradiation temperature or storage temperature is below 15°C. For thinner film and lower density of PE this effect appears clearly at the higher temperature. Therefore, it may be concluded that the low-temperature grafting of butadiene onto PE preirradiated in air is initiated predominantly by nonoxidized radical (allyl radical) trapped in PE and that the grafting reaction occurs in the semicrystalline part of PE. 相似文献
26.
An aqueous suspension system for phospholipase D-mediated synthesis of PS without toxic organic solvent 总被引:3,自引:2,他引:1
Yugo?Iwasaki Yukiko?Mizumoto Takahiro?Okada Takaya?Yamamoto Kentaro?Tsutsumi Tsuneo?YamaneEmail author 《Journal of the American Oil Chemists' Society》2003,80(7):653-657
Enzymatic synthesis of PS by phospholipase D (PLD)-mediated transphosphatidylation in an aqueous media was investigated. The
purpose of this study was to establish a novel synthetic method where no toxic organic solvents were used. An attempt to react
soybean lecithin (simply dispersed in an aqueous buffer) with an aqueous solution of l-serine and PLD was unsuccessful, giving only 20% of PS. By contrast, a suspension of lecithin adsorbed on fine powders such
as silica was effectively converted into PS in an aqueous solution of l-serine and PLD. After screening various powders for use as the lecithin adsorbent, calcium sulfate was found to be the best
with respect to lecithin conversion. In addition, calcium sulfate did not require prior adsorption of lecithin (i.e., the
reaction proceeded effectively simply by adding the powder to an aqueous mixture of lecithin, l-serine, and PLD). With this “aqueous suspension system” of calcium sulfate, up to 180 mg/mL lecithin was completely converted,
resulting in more than 80% PS in 24 h. The synthesized PS could easily be recovered from the powder by extracting with a mixture
of n-hexane, ethanol, and diluted HCl. 相似文献
27.
Maruyama T Shirota O Kawahara N Yokoyama K Makino Y Goda Y 《Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan》2003,44(1):44-48
'Magic mushrooms' (MMs) are psychoactive fungi containing the hallucinogenic compounds, psilocin (1) and psilocybin (2). Since June 6, 2002, these fungi have been regulated by the Narcotics and Psychotropics Control Law in Japan. Because there are many kinds of MMs and they are sold even as dry powders in local markets, it is very difficult to identify the original species of the MMs by morphological observation. Therefore, we investigated the internal transcribed spacer (ITS) region in the ribosomal RNA gene of MMs obtained in Japanese markets to classify them by a genetic approach. Based on the size and nucleotide sequence of the ITS region amplified by PCR, tested MMs were classified into 6 groups. Furthermore, a comparison of the DNA sequences of the MMs with those of authentic samples or with those found in the databases (GenBank, EMBL and DDBJ) made it possible to identify the species of tested MMs. Analysis by LC revealed that psilocin (1) was contained at the highest level in Panaeolus cyanescens among the MMs, but was absent in the Amanita species. 相似文献
28.
For the visualization of phase objects by use of a differentiation filter, the phase variation is changed into the intensity variation by differentiation, and then the differentiated image is integrated. In the method used in practice, the differentiated image has been recorded on a film, and then integrated by use of a filter. In this paper, however, the image differentiated by means of a filter is entered into a computer using a CCD camera and then integrated. As a result, the method provides rapid on-line processing. We have performed computer simulations and a detailed analysis of the differentiation filter and also provide the experimental results of three-dimensional image visualization. 相似文献
29.
Oikawa D Nakanishi T Nakamura Y Takahashi Y Yamamoto T Shiba N Tobisa M Takagi T Iwamoto H Tachibana T Furuse M 《Lipids》2003,38(6):609-614
This study investigated the influence of PUFA on the properties of mouse skin. Mice (3 wk old) were given free access to oils
high in linoleic acid, CLA, or DHA for 4 wk. At the end of the experiment, their skins were compared by both biochemical and
histological methods. No significant differences in lipid and collagen contents were detected among treatments, although the
FA composition in the skin was altered depending upon the FA composition of the supplemented oils. Electron microscopy revealed
that the subcutaneous tissue layers in the CLA and DHA groups were significantly thinner than that in the high linoleic acid
group, whereas no differences in the thickness of dermis layers were observed among the three groups. These results suggest
that skin properties in mice are readily modified by dietary FA sources within 4 wk of dietary oil supplementation. 相似文献
30.
Min‐Jung Bae Takeshi Ishii Kanako Minoda Yukiko Kawada Tatsuya Ichikawa Taiki Mori Miya Kamihira Tsutomu Nakayama 《Molecular nutrition & food research》2009,53(6):709-715
(–)‐Epigallocatechin gallate (EGCg) is the major component of green tea and is known to show strong biological activity, although it can be easily oxidized under physiological conditions. In this study, we indicate that EGCg is stable in human serum and that human serum albumin (HSA) stabilizes EGCg under aerobic condition. Although EGCg is usually decomposed within 1 h in aqueous solution at neutral pH, EGCg in serum and phosphate buffer (pH 7.4) containing HSA was stable over 1 h, even at neutral and slightly alkaline pH. Under these conditions, EGCg binds to HSA non‐covalently. The sulfhydryl group acts as an antioxidant for EGCg oxidation. Incubation of EGCg with HSA is accompanied by the oxidation of a free sulfhydryl group in HSA. These results suggest that the antioxidant property and the binding capacity of HSA contribute to the stabilization of EGCg in human serum. 相似文献