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141.
MJ Lizak EF Secchi JW Lee S Sato E Kubo Y Akagi PF Kador 《Canadian Metallurgical Quarterly》1998,39(13):2688-2695
PURPOSE: To investigate flux through the polyol pathway in the dog lens by 19F-nuclear magnetic resonance (19F-NMR) spectroscopy, using 3-fluoro-3-deoxy-D-glucose (3-FG) as a substrate. METHODS: 3-FG metabolism was monitored by 19F-NMR analysis. Dog lenses were incubated in Dulbecco's modified Eagle's medium containing 10 mM 3-FG. Enzymatic reductase and dehydrogenase activities were spectrophotometrically determined, whereas the analyses of 3-FG metabolites were conducted by 19F-NMR analysis. Aldose reductase (AR) was immunohistochemically localized in dog lens with antibodies raised against dog kidney AR. RESULTS: 19F-NMR spectra indicate that incubation of purified dog lenses AR with 3-FG results in the formation of 3-fluoro-3-deoxy-D-sorbitol (3-FS) and that incubation of dog liver sorbitol dehydrogenase (SDH) with 3-FS results in the formation of 3-fluoro-3-deoxy-D-fructose (3-FF). This confirms that 3-FG is metabolized to 3-FF by the polyol pathway enzymes. The affinity (Km) of AR for 3-FG is approximately 20-fold better than that for D-glucose, whereas the Km of SDH for 3-FS was fourfold less than for D-sorbitol. 3-FG in cultured dog lenses is metabolized primarily to 3-FS; however, small amounts of 3-FF and 3-fluoro-3-deoxy-D-gluconic acid (3-FGA) are also formed. 3-FS formation was reduced by the AR inhibitor AL 1576, and 3-FF formation was eliminated by the SDH inhibitor CP-166,572. In dog lens epithelial cells cultured with 3-FG, only 3-FS is formed. Similarly, only 3-FS is formed when lens capsule containing primarily epithelial lens contaminated with superficial epithelial cells was incubated in 3-FG. Similar incubation of the remaining cortex resulted primarily in the formation of 3-FS and 3-FGA. This enzymatic distribution was confirmed by spectrophotometric activity analysis and the immunohistochemical localization of AR. CONCLUSIONS: The data confirm that flux through the polyol pathway primarily results in sorbitol accumulation. The absence of fructose and gluconic acid from cultured lens epithelium suggests that the epithelial cells primarily contain AR, whereas differentiated fiber cells also contain SDH and glucose dehydrogenase. 相似文献
142.
Tyrosinase inhibitors from Anacardium occidentale fruits 总被引:1,自引:0,他引:1
Anacardic acids, 2-methylcardols, and cardols isolated from various parts of the cashew [Anacardium occidentale] (Anacardiaceae) fruit have been found to exhibit tyrosinase inhibitory activity. Kinetic studies with the two principal active compounds, 6-[8(Z),11(Z),14-pentadecatrienyl]salicylic acid and 5-[8(Z),11(Z),14-pentadecatrienyl]resorcinol, have indicated that both of these phenolic compounds exhibit characteristic competitive inhibition of the oxidation of L-3,4-dihydroxyphenylalanine (L-DOPA) by mushroom tyrosinase. 相似文献
143.
Hiroi Masato Hojo Masayuki Hashimoto Yukio Abe Yoshikazu Dote Yasuhiko 《Industrial Electronics, IEEE Transactions on》1986,(2):110-113
A methodology of discontinuous feedback and continuous feedforward control is developed to achieve accurate decoupled tracking in a class of nonlinear, time varying systems in the presence of disturbances, parameter variations and nonlinear dynamic interactions. The method is based on an improved variable structure control with a sliding mode. 相似文献
144.
Makoto Sakuragi Saki Tsuzuki Sei Obuse Akira Wada Kenji Matoba Izumi Kubo Yoshihiro Ito 《Materials science & engineering. C, Materials for biological applications》2010,30(2):316-322
A novel photoreactive polymer containing sulfobetaine polar groups was prepared by copolymerization of two kinds of methacrylic acids with sulfobetaine and azidoaniline. The polymer was photoimmobilized on polyester and polystyrene surfaces. Its effects on surface modification were investigated from its interactions with water, proteins and cells. Polymer immobilization altered both of the plain surfaces to becoming hydrophilic in a similar range of static contact angles (12.5 ± 1.6° on polyester and 14.7 ± 2.2° on polystyrene). This suggests that the surfaces were covered with sulfobetaine polar groups. Micropattern immobilization was carried out on both polymers using a photomask. The formed pattern was identical to the photomask, showing that the polymer was formed in response to ultraviolet irradiation. Measurements using atomic force microscopy showed that the polymer was formed at a thickness of 550 nm, demonstrating that the polymer was cross-linked with itself and with the substrate molecules. Measurements using time-of-flight secondary ion mass spectrometry detected an abundance of sulfur-containing ions in the patterned polymer, confirming that sulfobetaine had been immobilized. Protein adsorption and mammalian cell adhesiveness were reduced markedly on the immobilized regions. The reduction of cell adhesiveness was concentration-dependent for the immobilized polymer on polyester surfaces. In conclusion, a novel sulfobetaine-containing polymer was immobilized photoreactively on conventional polymer surfaces and significantly reduced interactions with proteins and mammalian cells. 相似文献
145.
146.
Bong-Hwan Oh Kazuaki Ishikawa Naoki Hayakawa Hitoshi Okubo Yukio Kito 《Electrical Engineering in Japan》1994,114(1):45-53
A superconducting multistranded cable is used to realize high current capacity for ac use. The critical current value of the cable is reported to be less than the simple summation of the individual critical current value of each strand. The causes for such a degradation of the critical current value have not been revealed. This paper investigates the current distribution in multistrands before and after their quenching by using seven-strand superconducting cable and 7x7 cable. The following experimental results are derived: (1) the quenching is initiated at one strand in the cable; (2) the current in the quenched strand is transferred into the other strands; (3) an avalanche of quenching is induced among the strands; and (4) the central strand is quenched finally among the strands. The critical current values of the 7- and 7 × 7-stranded cables also are measured. These values are in good agreement with the predicted values based on the mutual inductance among the strands. It is concluded that the unbalance of the current distribution in the superconducting multistrands can be one of the promising causes for the degradation of the critical current value. 相似文献
147.
148.
T Tsukamoto H Kinoshita K Hirohashi S Kubo S Otani 《Canadian Metallurgical Quarterly》1997,44(15):744-750
BACKGROUND/AIM: There are various indices of liver regeneration, but no clinically useful index that reflects the current status of liver regeneration. We assayed human erythrocyte polyamine levels after partial hepatectomy to define the relationship between erythrocyte polyamine levels and liver regeneration. MATERIALS AND METHODS: Levels of human erythrocyte polyamines (putrescine, spermidine, and spermine) were assayed by high-pressure liquid chromatography in 91 patients after partial hepatectomy and in 13 patients after surgery other than partial hepatectomy (controls). Of the patients after partial hepatectomy, 37 underwent hepatectomy of 20% or more of the liver (group A), 27 underwent segmentectomy or subsegmentectomy of the liver amounting to less than 20% of the liver (group B), and 27 underwent an operation smaller in scale than sub-segmentectomy (group C). RESULTS: The greater the proportion of the liver resected, the greater was the percent increase. In groups A, B, and C, erythrocyte levels of spermidine and spermine increased after surgery compared with the base line, and were significantly higher at 7 or 14 days, decreasing later. The differences in spermidine among the three groups were significant. CONCLUSIONS: After partial hepatectomy, the erythrocyte polyamine levels, especially the level of spermidine, were related to the proportion of liver resected. They seemed to reflect the degree of liver regeneration. 相似文献
149.
Masamitsu Kosaki Masayuki Nagao Yukio Mizuno Noriyuki Shimizu Kenji Horii 《Electrical Engineering in Japan》1991,111(6):67-75
A superconducting power cable is one of the promising ways of underground transmission of huge electric power in the future. The authors have long proposed the idea of the extruded polymer insulation for superconducting cables. The prominent features of the design are to exploit the excellent electrical properties of polymer in the cryogenic temperatures and to separate the helium coolant from the electrical insulation. Although the extruded cross-linked polyethylene cable has proved ability at the liquid nitrogen temperature, the cable insulation cracked due to mechanical stress during cooling to the liquid helium temperature. To overcome this problem, ethylene propylene rubber (EPR) was selected as a new insulating material considering the good results of mechanical and electrical tests of EPR samples at cryogenic temperatures. An extruded EPR insulated superconducting cable 15 m in length was fabricated and a cooling test down to the liquid helium temperature and a voltage test at the liquid helium temperature were carried out with fair success. This is a breakthrough in terms of the electrical insulation design of cryogenic cables. 相似文献
150.
Osamu Fujiwara Ikuko Mori Shinobu Ishigami Yukio Yamanaka 《Electrical Engineering in Japan》2005,153(1):17-24
An ESD (electrostatic discharge) testing is specified in the IEC 61000‐4‐2, in which the detailed waveform of the discharge current injected by an ESD gun is prescribed. However, due to lack of understanding of the discharge process, it is difficult to confirm whether or not the IEC current waveform can be injected onto actual equipment. We thus previously proposed an equivalent circuit model for the ESD gun based on its geometrical structure, and showed decisive factors for the discharge current. In this study, in order to confirm the feasibility of the above equivalent circuit model, we measured with a 6‐GHz wide‐band digital oscilloscope the discharge current through an SMA connector and the resultant magnetic near field for the contact discharge of an ESD gun. As a result, we found that both measured waveforms approximately agree with those calculated from our equivalent circuit model. We then measured with respect to charge voltages the magnetic near fields for the contact discharge of the ESD gun to the ground, which revealed that the measured waveform around the first peak is in fair agreement with the calculated one. Furthermore, we found that the magnetic field peak increases with increasing charge voltage, whose dependence can be predicted from our equivalent model. © 2005 Wiley Periodicals, Inc. Electr Eng Jpn, 153(1): 17–24, 2005; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/eej.20194 相似文献