Two-dimensional multiarray formation of hepatocyte spheroids on a microfabricated PEG-brush surface

A two-dimensional microarray of ten thousand (100 x 100) hepatocyte heterospheroids, underlaid with endothelial cells, was successfully constructed with 100 microm spacing in an active area of 20 x 20 mm on microfabricated glass substrates that were coated with poly(ethylene glycol) brushes. Cocultivation of hepatocytes with endothelial cells was essential to stabilize hepatocyte viability and liver-specific functions, allowing us to obtain hepatocyte spheroids with a diameter of 100 microm, functioning as a miniaturized liver to secret albumin for at least one month. The most important feature of this study is that these substrates are defined to provide an unprecedented control of substrate properties for modulating cell behavior, employing both surface engineering and synthetic polymer chemistry. The spheroid array constructed here is highly useful as a platform of tissue and cell-based biosensors and detects a wide variety of clinically, pharmacologically, and toxicologically active compounds through a cellular physiological response.     

Application of diamond-like Carbon (DLC) coatings for gravitational wave detectors

A laser interferometer gravitational wave detector requires an ultra high vacuum in the tubes in which the laser beams pass. To avoid noise due to scattered light from the surface of tubes, the surface should be treated with “black” coating without increasing the outgassing rate. We found that diamond-like Carbon (DLC) coatings are suitable for this aim. An outgassing rate of at was achieved for DLC coatings on SUS304 without baking. This rate was better than the surface of SUS316 with baking. The reflectivity of the DLC surface was 5% at minimum for a laser beam of Nd:YAG () which is used as a light source for the gravitational wave detector.     

Finite element iterative techniques for determining the interface boundary between Laplace and Poisson domains—characteristic analysis of a field effect transistor

Two kinds of techniques for solving a shape determination problem are proposed. The determination of the interface boundary between two domains governed by Poisson and Laplace equations under the compatible and constraint condition is considered. Influence coefficient and inverse variational approaches are examined by using the iterative finite element procedure. A two-dimensional model of a junction-type field effect transistor is a test example. The determination of its interface boundary and the prediction of the potential distribution and static characteristic are demonstrated.     

Copolymerization of glycidylmethacrylate–methylmethacrylate–divinylbenzene in cyclohexane

The copolymerization of glycidylmethacrylate–methylmethacrylate–divinylbenzene in cyclohexane was studied as an example of crosslinking precipitation copolymerization. The resultant copolymer is fine spheroidal. The copolymerization proceeds acceleratively at first, then steadily, and gradually slows down. The larger the divinylbenzene concentration, the smaller is the copolymerization rate. Glycidylmethacrylate is preferentially incorporated in the copolymer.     

Sliding window protocol with selective-repeat ARQ: performance modeling and analysis

A sliding window mechanism is well known to be a convenient way to integrate cumulative acknowledgement and flow control functions in a simple manner. Hence, widely used reliable data-transfer protocols such as TCP-SACK and RLC utilize this sliding window mechanism in conjunction with a selective-repeat ARQ (automatic repeat request) function, namely, the selective-repeat sliding window protocol (SR-SWP). We demonstrate that SR-SWP yields a throughput restraint effect (TORE) in particular situations when packet losses occur. We propose an analytical performance model to represent the TORE in the case of heavy traffic. Using this model, we derive explicit forms of goodput and mean resequencing delay, which are fundamental qualities of service parameters. From numerical results, we demonstrate that the impact of TORE on the performance is not negligible for high packet-loss probabilities. Furthermore, we show that the SR-SWP model exhibits the lower goodput and the smaller mean resequencing delay than those obtained from the traditional SR-ARQ protocol model.     

Speech recognition in living rooms: Integrated speech enhancement and recognition system based on spatial,spectral and temporal modeling of sounds

Research on noise robust speech recognition has mainly focused on dealing with relatively stationary noise that may differ from the noise conditions in most living environments. In this paper, we introduce a recognition system that can recognize speech in the presence of multiple rapidly time-varying noise sources as found in a typical family living room. To deal with such severe noise conditions, our recognition system exploits all available information about speech and noise; that is spatial (directional), spectral and temporal information. This is realized with a model-based speech enhancement pre-processor, which consists of two complementary elements, a multi-channel speech–noise separation method that exploits spatial and spectral information, followed by a single channel enhancement algorithm that uses the long-term temporal characteristics of speech obtained from clean speech examples. Moreover, to compensate for any mismatch that may remain between the enhanced speech and the acoustic model, our system employs an adaptation technique that combines conventional maximum likelihood linear regression with the dynamic adaptive compensation of the variance of the Gaussians of the acoustic model. Our proposed system approaches human performance levels by greatly improving the audible quality of speech and substantially improving the keyword recognition accuracy.     

Estimation of Lambert parameter based on leaf-scale hyperspectral images using dichromatic model-based PCA

Low-altitude hyperspectral observation systems are promising sensing tools for acquisition of optical remote-sensing data under the humid subtropical climate in Japan. The system is also capable of acquiring leaf-scale optical information free from atmospheric effect. However, the leaf-scale hyperspectral data are affected by shading and various illumination conditions such that it is difficult to obtain consistent characteristics of the spectral information. The aim of this article is the extraction of Lambert coefficients as an inherent leaf spectral profile. In this work, we propose a dichromatic model-based principal component analysis on hyperspectral data by utilizing leaf-scale hyperspectral data in order to diminish the spectral difference caused by the illumination condition and bidirectional reflectance distribution function. The results show that indices of chlorophyll content based on the estimated Lambert coefficients are consistent with the growth stages of a paddy field, whether the illumination condition is clear sky or overcast.     

Co-continuous monolithic titania prepared by organic polymer monolith as pore template

We achieved preparation of co-continuous titania based monolithic materials using several organic polymer monoliths as pore templates. Firstly, the organic polymer monoliths that had well controlled structures were prepared, and filled the pores of polymer monolith with tetra-n-butyl titanate (titan monomer). The following hydrolysis of the titan monomer resulted in titanium dioxide. The polymer monolith was removed by calcination at elevated temperature. We carefully studied the utility of polymer monolithic template, filling method of the titan monomer, and calcination conditions to realize co-continuous titania monolith. In addition, by the change of domain size (size of a skeleton + size of through a pore) of template, we were able to control domain size of the resulting titania monoliths.