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61.
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Correlations between the chemical structures of agricultural fungicides and mRNA expression levels following exposure of Saccharomyces cerevisiae to toxic doses of thiuram, zineb, maneb, TPN, and PCP were examined. Structurally, thiuram, zineb, and maneb are dithiocarbamate fungicides, whereas TPN and PCP are not. To characterize chemical toxicity, genes expression was classified according to the functional groups used by the MIPS database. However, no correlations between the classification scheme and chemical structures were found. Hierarchical clustering of gene expression profiles was performed to characterize the effects of the five chemicals. According to this analysis the similarity of gene expression profiles depended on the similarity of chemical structures. These results suggest that DNA microarray technology has potential for predicting the major chemicals which will cause environmental toxicity and will provide information on new biomonitoring methods. 相似文献
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Takeba K Fujinuma K Sakamoto M Jimbo K Oka H Ito Y Nakazawa H 《Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan》2003,44(5):246-252
A simple and rapid liquid chromatographic method was developed for the simultaneous determination of twelve benzimidazole anthelmintics in livestock foods using reversed-phase high-performance liquid chromatography with photodiode array detection (PDA). A sample was homogenized with acetonitrile and n-hexane, and centrifuged. The acetonitrile phase was isolated and evaporated. The residue was dissolved in 0.1 mol/L carbonate buffer solution (pH = 9.1), sonicated, and then subjected to clean-up on a Bond Elut LRC-C18 cartridge. The benzimidazole compounds were separated isocratically on a Capcell Pak C18 UG 120 (5 microns, 150 x 4.6 mm i.d.) column and detected by PDA at 295 and 313 nm. Mixtures of acetonitrile and 0.05 mol/L ammonium acetate in mixing ratios of (20:80) and (40:60) were used as the mobile phase, and the flow-rate was 1.0 mL/min at 40 degrees C. The mean recoveries (n = 3) from 0.1-0.5 microgram/g added samples were 72.6-97.2% with coefficients of variation of 0.3-8.5%. The detection limits were 0.01-0.05 microgram/g. 相似文献
65.
Takeshi Ando Eiichi Ohki Yasutaka Nakashima Yutaka Akita Hiroshi Iijima Osamu Tanaka 《Advanced Robotics》2013,27(15):1727-1746
The article describes the development of a gait phase time-based split-belt treadmill measurement system. Conventional methods of measuring gait phase, such as the foot switch and force plate, require significant preparation and are costly. In this article, we propose a simple, cheap, and accurate gait phase measurement system that utilizes only the treadmill motor current value. Comparison of this algorithm with conventional methods reveals that the proposed algorithm is as accurate as the foot switch. Moreover, the proposed algorithm can estimate stance phase within a 0.2?s error of the measured value of the force plate in most cases (four out of five healthy subjects). This accuracy is higher than that of the foot switch which is widely used in the clinical field. 相似文献
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This paper examines the effect of rule weights in fuzzy rule-based classification systems. Each fuzzy IF-THEN rule in our classification system has antecedent linguistic values and a single consequent class. We use a fuzzy reasoning method based on a single winner rule in the classification phase. The winner rule for a new pattern is the fuzzy IF-THEN rule that has the maximum compatibility grade with the new pattern. When we use fuzzy IF-THEN rules with certainty grades, the winner is determined as the rule with the maximum product of the compatibility grade and the certainty grade. In this paper, the effect of rule weights is illustrated by drawing classification boundaries using fuzzy IF-THEN rules with/without certainty grades. It is also shown that certainty grades play an important role when a fuzzy rule-based classification system is a mixture of general rules and specific rules. Through computer simulations, we show that comprehensible fuzzy rule-based systems with high classification performance can be designed without modifying the membership functions of antecedent linguistic values when we use fuzzy IF-THEN rules with certainty grades 相似文献
68.
Zhao Xiaobo Nakashima K. 《IEEE transactions on systems, man, and cybernetics. Part A, Systems and humans : a publication of the IEEE Systems, Man, and Cybernetics Society》2000,30(2):159-165
We consider a production system in a general configuration with a new control strategy: the push mechanism for the part transport and the kanban technique for the work-in-process (WIP). The production system is composed of many stations such as an entrance station, a set of work stations, a central station, and an exit station, that are arranged in a general configuration. The push mechanism is followed for transporting a part from a station to a destination station. The kanban technique is adopted for controlling the WIP in a work station. The production system is modeled by a closed queuing network in a general configuration with a Markov part sending mechanism and a machine no blocking (MNB) technique. An optimal part sending policy that maximizes the expected system throughput is formulated into a long run average semi-Markov decision process. Three solution approaches are developed for obtaining optimal or suboptimal solutions. Numerical examples are given to evaluate the quality of the solutions obtained by the solution approaches 相似文献
69.
Lanthanide(lII) chelates with 1,1,1 -trif luoro-5,5-dimethyl-2,4-hexanedione form adducts with benzilic acid, an a-hydroxycar-boxylic acid, in chloroform. The order of. the adduct formation constants in magnitude is: Lam> Eum> Ybm although it is known that the stability constant of lanthanide(III) complexes with α-hydroxycarboxylic acids in aqueous solutions increases with increasing atomic number. 相似文献
70.