Carbon Metabolism of a Soilborne Mn(II)-Oxidizing Escherichia coli Isolate Implicated as a Pronounced Modulator of Bacterial Mn Oxidation

Mn(II)-oxidizing microorganisms are generally considered the primary driving forces in the biological formation of Mn oxides. However, the mechanistic elucidation of the actuation and regulation of Mn oxidation in soilborne bacteria remains elusive. Here, we performed joint multiple gene-knockout analyses and comparative morphological and physiological determinations to characterize the influence of carbon metabolism on the Mn oxide deposit amount (MnODA) and the Mn oxide formation of a soilborne bacterium, Escherichia coli MB266. Different carbon source substances exhibited significantly varied effects on the MnODA of MB266. A total of 16 carbon metabolism-related genes with significant variant expression levels under Mn supplementation conditions were knocked out in the MB266 genome accordingly, but only little effect on the MnODA of each mutant strain was accounted for. However, a simultaneous four-gene-knockout mutant (namely, MB801) showed an overall remarkable MnODA reduction and an initially delayed Mn oxide formation compared with the wild-type MB266. The assays using scanning/transmission electron microscopy verified that MB801 exhibited not only a delayed Mn-oxide aggregate processing, but also relatively smaller microspherical agglomerations, and presented flocculent deposit Mn oxides compared with normal fibrous and crystalline Mn oxides formed by MB266. Moreover, the Mn oxide aggregate formation was highly related to the intracellular ROS level. Thus, this study demonstrates that carbon metabolism acts as a pronounced modulator of MnODA in MB266, which will provide new insights into the occurrence of Mn oxidation and Mn oxide formation by soilborne bacteria in habitats where Mn(II) naturally occurs.     

脉冲电流在改善金属凝固组织中的作用

综述了脉冲电流在改善金属凝固组织方面的研究现状。从理论上归纳和分析了脉冲电流在金属凝固过程中产生作用的主要机制。     

碳中和目标下电力需求预测体系构建及华东区域电力需求发展趋势研究

碳达峰、碳中和将加速全社会各行各业,尤其是能源系统的转型,而电力行业作为能源枢纽,能源领域在双碳转型过程中将对电力需求带来不可忽视的影响。该文立足于分析双碳目标对于能源电力的影响,挖掘双碳和电力需求的关联性,提出碳中和目标下分行业发展趋势分析思路,构建了一套适应于双碳目标下的电力电量预测方法体系;以华东区域作为典型案例,研究了华东区域碳达峰至碳中和过程中的电力需求发展趋势。研究结果表明,所构建的分析思路和方案体系科学可行,预测结论合理。     

服装材料悬垂伸长量与成衣样板修正量的研究

服装材料是成衣生产与制作的基本要素,其特性将直接影响成衣穿着的美观和舒适度,也影响成衣生产中工业样板的准确性.通过自制的模具(180°半圆模型)测量服装材料在悬垂状态下不同纱向、不同基础长度的悬垂伸长量,进行科学合理的量化分析,总结出服装材料悬垂伸长的规律.通过函数关系建立了服装材料悬垂伸长量和成衣样板修正量间的关系,为成衣样板的修正提供了科学的、可靠的依据,提高了成衣工业样板的制作效率和结构、规格的精度.     

Differential MicroRNA Expression in Porcine Endometrium Related to Spontaneous Embryo Loss during Early Pregnancy

Litter size is an important indicator to measure the production capacity of commercial pigs. Spontaneous embryo loss is an essential factor in determining sow litter size. In early pregnancy, spontaneous embryo loss in porcine is as high as 20–30% during embryo implantation. However, the specific molecular mechanism underlying spontaneous embryo loss at the end of embryo implantation remains unknown. Therefore, we comprehensively used small RNA sequencing technology, bioinformatics analysis, and molecular experiments to determine the microRNA (miRNA) expression profile in the healthy and arresting embryo implantation site of porcine endometrium on day of gestation (DG) 28. A total of 464 miRNAs were identified in arresting endometrium (AE) and healthy endometrium (HE), and 139 differentially expressed miRNAs (DEMs) were screened. We combined the mRNA sequencing dataset from the SRA database to predict the target genes of these miRNAs. A quantitative real-time PCR assay identified the expression levels of miRNAs and mRNAs. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed on differentially expressed target genes of DEMs, mainly enriched in epithelial development and amino acids metabolism-related pathways. We performed fluorescence in situ hybridization (FISH) and the dual-luciferase report gene assay to confirm miRNA and predicted target gene binding. miR-205 may inhibit its expression by combining 3′-untranslated regions (3′ UTR) of tubulointerstitial nephritis antigen-like 1 (TINAGL1). The resulting inhibition of angiogenesis in the maternal endometrium ultimately leads to the formation of arresting embryos during the implantation period. This study provides a reference for the effect of miRNA on the successful implantation of pig embryos in early gestation.     

移动IPv6安全绑定更新机制研究

介绍了移动IPv6网络中节点移动后进行三角路由优化所引出的绑定更新安全问题,阐述了在非PKI体系中解决绑定更新安全隐患的几种机制;在阐述的同时对这些机制进行了安全性分析,最后对这几种机制从安全性角度进行比较.     

Functionalization of Tailored Porous Carbon Monolith for Decontamination of Radioactive Substances

As the control over radioactive species becomes critical for the contemporary human life, the development of functional materials for decontamination of radioactive substances has also become important. In this work, a three-dimensional (3D) porous carbon monolith functionalized with Prussian blue particles was prepared through removal of colloidal silica particles from exfoliated graphene/silica composite precursors. The colloidal silica particles with a narrow size distribution were used to act a role of hard template and provide a sufficient surface area that could accommodate potentially hazardous radioactive substances by adsorption. The unique surface and pore structure of the functionalized porous carbon monolith was examined using electron microscopy and energy-dispersive X-ray analysis (EDS). The effective incorporation of PB nanoparticles was confirmed using diverse instrumentations such as X-ray diffraction (XRD), Fourier-transform infrared (FT-IR), and X-ray photoelectron spectroscopy (XPS). A nitrogen adsorption/desorption study showed that surface area and pore volume increased significantly compared with the starting precursor. Adsorption tests were performed with ¹³³Cs ions to examine adsorption isotherms using both Langmuir and Freundlich isotherms. In addition, adsorption kinetics were also investigated and parameters were calculated. The functionalized porous carbon monolith showed a relatively higher adsorption capacity than that of pristine porous carbon monolith and the bulk PB to most radioactive ions such as ¹³³Cs, ⁸⁵Rb, ¹³⁸Ba, ⁸⁸Sr, ¹⁴⁰Ce, and ²⁰⁵Tl. This material can be used for decontamination in expanded application fields.     

深度双重注意力的生成与判别联合学习的行人重识别

在行人重识别任务中存在数据集标注难度大,样本量少,特征提取后细节特征缺失等问题。针对以上问题提出深度双重注意力的生成与判别联合学习的行人重识别。首先,构建联合学习框架,将判别模块嵌入生成模块,实现图像生成和判别端到端的训练,及时将生成图像反馈给判别模块,同时优化生成模块与判别模块。其次,通过相邻的通道注意力模块间连接和相邻空间注意力模块间连接,融合所有通道特征和空间特征,构建深度双重注意力模块,将其嵌入教师模型,使模型能更好地提取行人细节身份特征,提高模型识别能力。实验结果表明,该算法在Market-1501和DukeMTMC-ReID数据集上具有较好的鲁棒性、判别性。