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61.
E Noguchi N Hayashi Y Azuma T Seki M Nakamura N Nakashima M Yanagida X He U Mueller S Sazer T Nishimoto 《Canadian Metallurgical Quarterly》1996,15(20):5595-5605
Using the two-hybrid method, we isolated a Saccharomyces cerevisiae cDNA encoding a protein homologous to Schizosaccharomyces pombe protein Dis3sp, using as bait, human GTPase Ran. The DIS3 gene is essential for viability and complements S.pombe mutant dis3-54 which is defective in mitosis. Although Dis3sc has no homology to RanBP1, it bound directly to Ran and the S.cerevisiae Ran homologue Cnr1, but not to the S.cerevisiae RCC1 homologue Srm1. Upon binding to Ran with a 1:1 molar ratio, Dis3sc enhanced a nucleotide-releasing activity of RCC1 on Ran. In the presence of Dis3sc, the K(m) of RCC1 on Ran decreased by half, while the kcat was unchanged. In vivo, Dis3sp was present as oligomers of M(r) 670-200 kDa as previously reported, and the 200 kDa oligomer of Dis3sp was found to include Spi1 and Pim1, the S.pombe homologues of Ran and RCC1, respectively. Although the biological function of the heterotrimeric oligomer consisting of Dis3, Spi1 and Pim1 is unknown, our results indicate that Dis3 is a component of the RCC1-Ran pathway. 相似文献
62.
Fukuyama Y Yasuda N Kim J Murayama H Ohshima T Tanaka Y Kimura S Kamioka H Moritomo Y Toriumi K Tanaka H Kato K Ishikawa T Takata M 《The Review of scientific instruments》2008,79(4):045107
An ultra-high-precision clock system for long time delay has been developed for picosecond time-resolved x-ray diffraction measurements using synchrotron radiation (SR) pulses and synchronized femtosecond laser pulses. The time delay control between pump laser pulse and the probe SR pulse was achieved by combining an in-phase quadrature modulator and a synchronous counter. This method allowed us to change the delay time by a nearly infinite amount while maintaining the precision of +/-8.40 ps. Time-resolved diffraction measurements using the delay control system were demonstrated for precise measurement of an acoustic velocity in a single crystal of gallium arsenide. 相似文献
63.
K Hashimoto C Azuma Y Tokugawa T Nobunaga TA Aki Y Matsui T Yanagida H Izumi F Saji Y Murata 《Canadian Metallurgical Quarterly》1997,28(7):862-865
An unusual clinical presentation of a patient with neuronal intestinal dysplasia is presented. A 46-year-old male noted a palpable mass in the right lower quadrant of his abdomen for two months. A computed axial tomographic scan showed a thickened wall of the cecum with a tumor-like appearance. The excised specimen consisted of a mass caused by the thickened, edematous wall of the dilated cecum and appendix. The wall of the cecum and appendix measured up to 2.5 and 0.8 cm, respectively, in thickness. Microscopic studies showed extensive hyperplasia and hypertrophy of the ganglia and nerve plexuses and hypertrophy of the muscularis propria, consistent with neuronal intestinal dysplasia. 相似文献
64.
Taizo Sano Masamichi Tsuji Nobuyuki Gokon Hiroshi Kaneko Yutaka Tamaura 《Journal of the American Ceramic Society》2000,83(10):2509-2514
Oxygen uptake and release of (Li,Mn) ferrite [LMF; (Li0.60 Mn1.20 Fe1.20 )1−δ O4 ; δ= 0.007 to 0.033] was investigated concerning the relations between redox reactions of ferrite and cation migration. Mössbauer spectroscopy and X-ray diffractometry showed that some of the Li+ and Fe3+ ions migrated from the A sites to the B sites of the spinel-type structure and Mn4+ ions migrated from the B sites to the A sites during oxygen uptake at 573 K. The cation-deficient LMF formed by the oxygen uptake released oxygen molecules in He gas only at 660 K. The cation migration during the oxygen release was in the opposite direction of the movement during oxygen uptake at 573 K. 相似文献
65.
Chlorophyll-a (Chl-a) assembled in hydrophobic domain by fatty acid with long alkyl hydrocarbon chain such as myristic acid (Myr), stearic acid (Ste) and cholic acid (Cho) modified onto nanocrystalline TiO2 electrode is prepared and the photovoltaic properties of the nanocrystalline TiO2 film by Chl-a are studied. Incident photon to current efficiency (IPCE) value at 660 nm in photocurrent action spectrum of Chl-a/Ste-TiO2, Chl-a/Myr-TiO2 and Chl-a/Cho-TiO2 electrodes are 5.0%, 4.1% and 4.1%, respectively. Thus, the IPCE is maximum using Chl-a/Ste-TiO2 electrode. From the results of photocurrent responses with light intensity of 100 mW cm−2 irradiation or monochromatic light with 660 nm, generated photocurrent increases using Chl-a/Ste-TiO2 electrode compared with the other Chl-a assembled TiO2 electrodes. These results show that the hydrophobic domain formed by stearic acid with long alkyl hydrocarbon chain is suitable for fixation of Chl-a onto TiO2 film electrodes and photovoltaic performance is improved using Chl-a onto Ste-TiO2 film electrode. 相似文献
66.
High performance liquid chromatographic separation of monoacylglycerol enantiomers as di-3,5-dinitrophenylurethane derivatives
was carried out on a chiral stationary phase, N-(S)-2-(4-chlorophenyl)isovaleroyl-D-phenylglycine chemically bonded tov-aminopropyl silanized silica. Complete separation of the urethane derivatives of racemic monoacylglycerols with saturated
acyl groups of C12−C18 was achieved using a stainless steel column (25 cm long) packed with the 5μ particles, an isocratic elution at ambient temperature
with a mixture of hexane/ethylene dichloride/ethanol as a mobile phase, and a UV detector. Thesn-1 enantiomers were eluted ahead of the correspondingsn-3 enantiomers. Complete separation of thesn-2 isomers from the corresponding enantiomers and partial separation of the enantiomer homologues differing by two acyl carbons
also were observed. 相似文献
67.
Simultaneous extraction by microwave-irradiation and crystallisation were performed in the same pot of solvent of 70% (v/v) aqueous ethanol for isolation of hesperidin from thinned immature fruit peels of Citrus unshiu as refining of Citrus waste biomass. The hesperidin content in immature fruits peels was about 3.2-fold higher than that of mature fruit. After microwave-assisted extraction (MAE), the yield of hesperidin reached 58.6 mg/g, which was comparable to the amount obtained after extraction using DMSO:methanol (1:1, v/v) as a solvent for 30 min at room temperature. Heating temperature and time for isolation of hesperidin crystallites were optimised as 140 °C and 8 min by using response surface methodology. Under this optimal condition, 86.8% (47.7 mg/g) of total hesperidin was isolable by MAE and low-temperature storage (5 °C, 24 h). 相似文献
68.
Satoshi Suzuki Mari Fukuoka Hikaru Ookuchi Motoaki Sano Kenji Ozeki Emi Nagayoshi Yukio Takii Mayumi Matsushita Sawaki Tada Ken-Ichi Kusumoto Yutaka Kashiwagi 《Journal of Bioscience and Bioengineering》2010,109(2):115-117
This is the first report of glycoside hydrolase family 43 β-xylosidase from Aspergillus oryzae. To characterize this enzyme, the recombinant enzyme was expressed in Escherichia coli. Unlike known β-xylosidases from fungal origins, the enzyme did not show substrate ambiguity and was stable at alkaline pH. 相似文献
69.
Ethanol and lactic acid production using sap squeezed from old oil palm trunks felled for replanting
Akihiko Kosugi Ryohei Tanaka Kengo Magara Yoshinori Murata Takamitsu Arai Othman Sulaiman Rokiah Hashim Zubaidah Aimi Abdul Hamid Mohd Khairul Azri Yahya Mohd Nor Mohd Yusof Wan Asma Ibrahim Yutaka Mori 《Journal of Bioscience and Bioengineering》2010,110(3):322-325
Old oil palm trunks that had been felled for replanting were found to contain large quantities of high glucose content sap. Notably, the sap in the inner part of the trunk accounted for more than 80% of the whole trunk weight. The glucose concentration of the sap from the inner part was 85.2 g/L and decreased towards the outer part. Other sugars found in relatively low concentrations were sucrose, fructose, galactose, xylose, and rhamnose. In addition, oil palm sap was found to be rich in various kinds of amino acids, organic acids, minerals and vitamins. Based on these findings, we fermented the sap to produce ethanol using the sake brewing yeast strain, Saccharomyces cerevisiae Kyokai no.7. Ethanol was produced from the sap without the addition of nutrients, at a comparable rate and yield to the reference fermentation on YPD medium with glucose as a carbon source. Likewise, we produced lactic acid, a promising material for bio-plastics, poly-lactate, from the sap using the homolactic acid bacterium Lactobacillus lactis ATCC19435. We confirmed that sugars contained in the sap were readily converted to lactic acid with almost the same efficiency as the reference fermentation on MSR medium with glucose as a substrate. These results indicate that oil palm trunks felled for replanting are a significant resource for the production of fuel ethanol and lactic acid in palm oil-producing countries such as Malaysia and Indonesia. 相似文献
70.