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61.
62.
Low rates of spontaneous G:C-->C:G transversions would be achieved not only by the correction of base mismatches during DNA replication but also by the prevention and removal of oxidative base damage in DNA. Escherichia coli must have several pathways to repair such mismatches and DNA modifications. In this study, we attempted to identify mutator loci leading to G:C-->C:G transversions in E.coli. The strain CC103 carrying a specific mutation in lacZ was mutagenized by random miniTn 10 insertion mutagenesis. In this strain, only the G:C-->C:G change can revert the glutamic acid at codon 461, which is essential for sufficient beta-galactosidase activity to allow growth on lactose. Mutator strains were detected as colonies with significantly increased rates of papillae formation on glucose minimal plates containing P-Gal and X-Gal. We screened approximately 40 000 colonies and selected several mutator strains. The strain GC39 showed the highest mutation rate to Lac+. The gene responsible for the mutator phenotypes, mut39 , was mapped at around 67 min on the E.coli chromosome. The sequencing of the miniTn 10 -flanking DNA region revealed that the mut39 was identical to the mutY gene of E.coli. The plasmid carrying the mutY + gene reduced spontaneous G:C-->T:A and G:C-->C:G mutations in both mutY and mut39 strains. Purified MutY protein bound to the oligonucleotides containing 7,8-dihydro-8-oxo-guanine (8-oxoG):G and 8-oxoG:A. Furthermore, we found that the MutY protein had a DNA glycosylase activity which removes unmodified guanine from the 8-oxoG:G mispair. These results demonstrate that the MutY protein prevents the generation of G:C-->C:G transversions by removing guanine from the 8-oxoG:G mispair in E.coli. 相似文献
63.
Nakahara K. Nagaishi H. Hasegawa H. Kominami S. Yamada H. Nishino T. 《Applied Superconductivity, IEEE Transactions on》1994,4(4):223-227
An optical input/output interface system for a Josephson junction integrated circuit is fabricated and tested. The system consists of a superconducting optical detector, a dc powered Josephson circuit, a dc powered Josephson high voltage circuit, a liquid-He-cooled semiconductor amplifier, and a liquid-He-cooled semiconductor laser. Features of the system are use of an ultrathin NbN film for the optical detector and adoption of the dc powered Josephson circuits for logic operation circuits. Correct optical output signal is detected by a liquid-He-cooled semiconductor photodiode. The optical input/output interface has the advantage of low heat penetration and low crosstalk compared to the interface using conventional coaxial lines. Moreover, dc powered Josephson circuits have an advantage of low crosstalk from power supply lines compared to conventional Josephson circuits, which are driven by ac supply current 相似文献
64.
R. H. Kratzer K. L. Paciorek J. Kaufman J. H. Nakahara A. M. Hartstein 《应用聚合物科学杂志》1977,21(8):2077-2084
Uncured styrene–butadiene gum, a known cured composition, and four SBR beltings used in underground coal mining were subjected to thermal oxidative degradation in static and dynamic systems. The room temperature volatiles produced under the static conditions at 370°C were identified and quantitated. Monomers and their interaction products were liberated by the raw gum; the cured composition formed together with the above products materials traceable to the curing ingredients. The four beltings gave a large spectrum of products; an attempt was made to correlate these with possible components used in belts manufacture. In the dynamic system, the occurrence of glow, the rate of smoke generation, and char yields were determined at 400°C and 500°C. None of the materials glowed at 400°C. At 500°C, the raw gum volatilized completely, and the cured rubber and two beltings glowed. One of the beltings, which failed to glow, liberated the smallest quantity of toxic products. 相似文献
65.
Transmission with a BER less than 10?11 has been achieved using an injection-locking technique with a 445.8 Mbit/s RZ signal over a 170 km single-mode fibre. The injection-locking technique provides a single-longitudinal-mode optical source with a 35 dB main/side-mode power ratio and a 1.5 GHz spectral width. 相似文献
66.
The OSE Project aims at developing the technology to utilize solar energy for heating, cooling and hot water supply on the basis of various technology for energy conservation in buildings. For the first step of this project a solar heating and cooling system with flat plate collectors and absorption refrigeration machine was installed in a house in 1974. This paper reports outlines of the system and operating results. 相似文献
67.
Fisher information has been used to analyze the accuracy of neural population coding. This works well when the Fisher information does not degenerate, but when two stimuli are presented to a population of neurons, a singular structure emerges by their mutual interactions. In this case, the Fisher information matrix degenerates, and the regularity condition ensuring the Cramér-Rao paradigm of statistics is violated. An animal shows pathological behavior in such a situation. We present a novel method of statistical analysis to understand information in population coding in which algebraic singularity plays a major role. The method elucidates the nature of the pathological case by calculating the Fisher information. We then suggest that synchronous firing can resolve singularity and show a method of analyzing the binding problem in terms of the Fisher information. Our method integrates a variety of disciplines in population coding, such as nonregular statistics, Bayesian statistics, singularity in algebraic geometry, and synchronous firing, under the theme of Fisher information. 相似文献
68.
69.
Kazuhiro Hirano Hideki Yamauchi Naoya Nakahara Kazuo Kinoshita Maki Yamaguchi Shigeru Takemori 《International journal of molecular sciences》2021,22(23)
We performed X-ray diffraction analyses on rat plantaris muscle to determine if there are strain-specific structural changes at the molecular level after eccentric contraction (ECC). ECC was elicited in situ by supramaximal electrical stimulation through the tibial nerve. One hour after a series of ECC sessions, the structural changes that remained in the sarcomere were evaluated using X-ray diffraction. Proteins involved in cell signaling pathways in the muscle were also examined. ECC elicited by 100, 75, and 50 Hz stimulation respectively developed peak tension of 1.34, 1.12 and 0.79 times the isometric maximal tetanus tension. The series of ECC sessions phosphorylated the forkhead box O proteins (FoxO) in a tension-time integral-dependent manner, as well as phosphorylated the mitogen-activated protein kinases (MAPK) and a protein in the mammalian target of rapamycin (mTOR) pathway in a maximal tension dependent manner. Compared to isometric contractions, ECC was more efficient in phosphorylating the signaling proteins. X-ray diffraction revealed that the myofilament lattice was preserved even after intense ECC stimulation at 100 Hz. Additionally, ECC < 75 Hz preserved the molecular alignment of myoproteins along the myofilaments, while 75-Hz stimulation induced a slight but significant decrease in the intensity of meridional troponin reflection at 1/38 nm−1, and of myosin reflection at 1/14.4 nm−1. These two reflections demonstrated no appreciable decrease with triple repetitions of the standard series of ECC sessions at 50 Hz, suggesting that the intensity decrease depended on the instantaneous maximal tension development rather than the total load of contraction, and was more likely linked with the phosphorylation of MAPK and mTOR signaling proteins. 相似文献
70.
The stochastic mechanism of synchronous firing in a population of neurons is studied from the point of view of information geometry. Higher-order interactions of neurons, which cannot be reduced to pairwise correlations, are proved to exist in synchronous firing. In a neuron pool where each neuron fires stochastically, the probability distribution q(r) of the activity r, which is the fraction of firing neurons in the pool, is studied. When q(r) has a widespread distribution, in particular, when q(r) has two peaks, the neurons fire synchronously at one time and are quiescent at other times. The mechanism of generating such a probability distribution is interesting because the activity r is concentrated on its mean value when each neuron fires independently, because of the law of large numbers. Even when pairwise interactions, or third-order interactions, exist, the concentration is not resolved. This shows that higher-order interactions are necessary to generate widespread activity distributions. We analyze a simple model in which neurons receive common overlapping inputs and prove that such a model can have a widespread distribution of activity, generating higher-order stochastic interactions. 相似文献