Nonlinear black-box modeling in system identification: a unified overview

A nonlinear black-box structure for a dynamical system is a model structure that is prepared to describe virtually any nonlinear dynamics. There has been considerable recent interest in this area, with structures based on neural networks, radial basis networks, wavelet networks and hinging hyperplanes, as well as wavelet-transform-based methods and models based on fuzzy sets and fuzzy rules. This paper describes all these approaches in a common framework, from a user's perspective. It focuses on what are the common features in the different approaches, the choices that have to be made and what considerations are relevant for a successful system-identification application of these techniques. It is pointed out that the nonlinear structures can be seen as a concatenation of a mapping form observed data to a regression vector and a nonlinear mapping from the regressor space to the output space. These mappings are discussed separately. The latter mapping is usually formed as a basis function expansion. The basis functions are typically formed from one simple scalar function, which is modified in terms of scale and location. The expansion from the scalar argument to the regressor space is achieved by a radial- or a ridge-type approach. Basic techniques for estimating the parameters in the structures are criterion minimization, as well as two-step procedures, where first the relevant basis functions are determined, using data, and then a linear least-squares step to determine the coordinates of the function approximation. A particular problem is to deal with the large number of potentially necessary parameters. This is handled by making the number of ‘used’ parameters considerably less than the number of ‘offered’ parameters, by regularization, shrinking, pruning or regressor selection.     

Human chromosome 5 sequence primer amplifies Alu polymorphisms on chromosomes 2 and 17

Members of the Alu family of repetitive elements occur frequently in the human genome and are often polymorphic. Techniques involving Alu element mediated polymerase chain reactions (Alu PCR) allow the isolation of region-specific human DNA fragments from mixed DNA sources. Such fragments are a source of region-specific Alu elements useful for the detection of Alu-related polymorphisms. A clone from human chromosome 5, corresponding to locus D5F40S1, was isolated using Alu PCR differential hybridization. Alu elements within this clone were investigated for the presence of potentially polymorphic 3' polyA tails. Primers were devised to amplify the 3' polyA tail of an Alu element present within the clone. One primer, D5F40S1-T, was specific to the DNA flanking the 3' end of the Alu element, and the other primer was homologous to sequences within the element. When these primers were used in PCR reactions, products from chromosomes 2 and 17 (loci D2F40S2 and D17F40S3) were amplified in addition to the expected product from chromosome 5. The most likely explanation for this nonspecific amplification is that the D5F40S1-T primer is located within a low-copy repetitive element that is 3' of the Alu element. This phenomenon presents a potential problem for the identification of region-specific Alu polymorphisms.     

Absorbing surface impedances of lossy layers in the finite difference time-domain method

In this paper the time-domain surface impedances of an homogeneous absorber layer, are given for the vertical and horizontal polarizations, or respectively for the electric field perpendicular or parallel to the incidence plane. It turns out that the application of the concept in finite difference time-domain (FDTD) in absorbing surface impedances boundary conditions, gives results in good agreement with analytical Fresnel reflection coefficients.     

Dynex: multiplex ELISA technology

Conventional enzyme-linked immunosorbent assay (ELISA) is a gold standard for screening antibodies and testing for protein or antigen presence. A significant limitation of this assay resides in the fact that only one analyte can be assessed per microplate well. Here, we describe and investigate a new technology consisting of an automated ELISA system in which up to 10 analytes can be measured within one single well, thus improving productivity, accuracy, and repeatability by reducing the amount of human labor required. Another strength of the platform is that a user can load any necessary sets/subsets of beads to perform required assays, with improved flexibility compared to manufactured-loaded arrays for multiplex analysis. We also demonstrate that this system can be used to determine the pathogenicity (i.e., presence of Shiga toxins) and serotype (i.e., Escherichia coli O157) of E. coli isolates.     

High nutrient loading and climatic parameters influence the dominance and dissimilarity of toxigenic cyanobacteria in northern bays of Lake Victoria

Eutrophication of Lake Victoria led to changes in its phytoplankton communities. However, different levels of eutrophication exist in the open lake and the bays, and between embayments. This study utilized spatial and temporal sampling of Napoleon Gulf and Murchison Bay, exhibiting different trophic conditions. Over one year, we investigated phytoplankton biomass, richness, diversity and dissimilarity, and related the dynamics of the dominant species to the limnological and climatic conditions. The results confirmed that Napoleon Gulf and Murchison Bay showed large differences in eutrophication status, with lower nutrient concentrations in Napoleon Gulf than in Murchison Bay, where a strong gradient was observed from inshore to offshore areas. These nutrient dynamics resulted in a 4 to 10 fold higher chlorophyll-a in Murchison Bay than in Napoleon Gulf. From the embayments, 135 phytoplankton taxa were recorded with no significant differences in alpha diversity. However, high dissimilarity in community structure was observed in beta diversity, mostly due to a turnover among the dominant toxigenic species. Thus, from a similar species pool, there was a shift in the dominant toxigenic cyanobacteria from Microcystis flos-aquae and M. aeruginosa in Murchison Bay, Dolichospermum circinale and Planktolyngbya circumcreta in Napoleon Gulf to D. circinale in the offshore stations. These cyanobacteria are toxigenic taxa with known health hazards. Using partial least square models, we showed that both climatic variables (e.g. wind, solar radiation) and levels of inorganic dissolved nutrients (e.g. SRP, NO₃^–, and NH₄⁺) are the main drivers of differences and dominance in cyanobacteria communities in northern Lake Victoria.     

Data stream classification with novel class detection: a review,comparison and challenges

Knowledge and Information Systems - Developing effective and efficient data stream classifiers is challenging for the machine learning community because of the dynamic nature of data streams. As a...     

A URI-based approach for addressing fragments of media resources on the Web

To make media resources a prime citizen on the Web, we have to go beyond simply replicating digital media files. The Web is based on hyperlinks between Web resources, and that includes hyperlinking out of resources (e.g., from a word or an image within a Web page) as well as hyperlinking into resources (e.g., fragment URIs into Web pages). To turn video and audio into hypervideo and hyperaudio, we need to enable hyperlinking into and out of them. The W3C Media Fragments Working Group is taking on the challenge to further embrace W3C??s mission to lead the World Wide Web to its full potential by developing a Media Fragment protocol and guidelines that ensure the long-term growth of the Web. The major contribution of this paper is the introduction of Media Fragments as a media-format independent, standard means of addressing media resources using URIs. Moreover, we explain how the HTTP protocol can be used and extended to serve Media Fragments and what the impact is for current Web-enabled media formats.     

TEM characterization of some crude or air heat-treated SiC Nicalon fibres

Commercial Nicalon fibres were prepared by thin transverse sectioning and studied by transmission electron microscopy. A progressive tilting of the incident beam allows us to explore the selected-area diffraction (SAD) pattern along two orthogonal directions, increasing the tilting angle (dark-field (DF) imaging). The lattice fringes technique was also used. The samples were Nicalon 001, 101 and 201 fibres, the latter also being studied after heat treatment in air at 1300° C for 48 h. The SAD pattern of the 001 fibre only shows the SiC, 1 1 intense halo whereas the other samples show all the SiC (1 1 1, 2 2 0 and 31 1) strongly scattered beams, indicating a microcrystalline state. Correspondingly, DF imaging does not indicate any localized measurable scattering domain for 001. Only bright dots can be seen, less than 1 nm in size. The other fibres show SiC microcrystals respectively 2 nm (1 01 ), 3 nm (201 ) and up to 7 nm (heat-treated 201) in extent. Free aromatic carbon, shaped in small units less than 1 nm in size fills up the interstices between SiC. These units tend to lie flat on SiC. In heat-treated fibres, they form incomplete layers around the edges. In addition, the heat-treated 2 01 fibre show a 1m thick layer of cristobalite at the fibre surface. These crystals are polytypes.     

Conception assistée par ordinateur des circuits intégrés monolithiques hyperfréquences

Computer aided design of microwave monolithic integrated circuits must combine two different fields. The first domain is the simulation of microwave circuits taking into account non linear aspects development of time domain and harmonic balance simulation algorithms specialized for microwave circuits. In the second place are found, the layout algorithms which were developed for logic circuits (on Si or GaAs). The association of these two types of algorithms leads to the constitution of aCad workstation for microwave monolithic integrated circuits. Two examples of design and realization of integrated circuits with these tools are presented.