Cytokines and PGE2 modulate the phagocytic function of the beta-glucan receptor in macrophages

Under serum-free conditions the beta-glucan receptor of mouse macrophages mediates phagocytosis of beta-1,3-D-glucan-coated microbeads (diameter 2 microns). IFN-gamma increases the phagocytic function of the beta-glucan receptor in a dose-dependent manner, giving the plateau level at 100 U/ml. Maximum activity appears 9 h after addition of IFN-gamma to the cells. The effect disappears within 24 h. The effect of IFN-gamma may be a result of augmented receptor synthesis since treatment with cycloheximide reduces the phagocytosis. IL-1 also increases the phagocytic function of the beta-glucan receptor giving a dose-dependent response and with the plateau level reached at 10 U/ml. Maximum activity is found 4 h after addition of IL-1 to macrophages. The effect disappears within 24 h. TNF does not alter the phagocytic function of the beta-glucan receptor, but TNF together with IL-1 prolongs the effect of IL-1. PGE2 reduces the phagocytic function of the beta-glucan receptor. Maximum reduction is achieved with 8 ng/ml. Time-course studies show the lowest phagocytic activity 9 h after addition of PGE2 to the cells.     

Synthesis and biological studies on analogs of cyclolinopeptide A with a shortened peptide chain

Continuing our work on the immunosuppressive activity of cyclolinopeptide A (CLA), a cyclic nonapeptide of the sequence: c-(-Leu-Ile-Ile-Leu-Val-Pro-Pro-Phe-Phe), we synthesized a series of 6 linear analogs of CLA, successively shortened at the N-terminus of the peptide chain. Immunosuppressive activity of acetates as well as trifluoroacetates of these peptides was investigated using PFC (humoral immunity) and DTH (cellular immunity) tests. It was found that the immunosuppressive potency of the peptides examined diminishes with shortening of the peptide chain. Octapeptide I with the sequence Ile-Ile-Leu-Val-Pro-Pro-Phe-Phe was found to be the most active of the whole series. The immunosuppressive activity increased again for a tripeptide fragment of CLA of the sequence Pro-Phe-Phe. The immunosuppressive activity of octapeptide probably depends on the suppression of IL-1, IL-6 and TNF production by the cells involved in immune and inflammatory response.     

An automatic machine for bending a furniture fitting tie rod

Translated from Khimicheskoe i Neftyanoe Mashinostroenie, No. 8, pp. 31–32, August, 1992.     

Lead acid battery storage configurations for improved availablecapacity

Battery storage is a crucial element in alternative energy and electric vehicle systems. Three battery storage configurations: a conventional; a parallel; and a dual, were analyzed for both shallow cycle and deep cycle lead-acid batteries to determine if capacity improvement is achievable. Daily profiles for the weekly irradiance, daily loads, and ambient temperature are simulated. Cycle tests were performed monthly to determine the effect each configuration had on the available battery capacity. Results for each battery type differ. Available capacity was improved using the parallel configuration with shallow cycle batteries and the dual configuration with deep cycle batteries     

An electrical network approach to the analysis of semiconductordevices

A simple electrical network is used to represent the five differential equations describing basic phenomena in one-dimensional semiconductor devices. Both standard and integrated approaches for solving transport equations are developed. Also, an electrical network equivalent for the nonlinear Poisson equation was derived. It allows students to better understand the physical phenomena and the process of computer simulation of such semiconductor devices     

Pneumothorax. A complication of fine needle aspiration of breast tumors

Of 1,666 patients who underwent fine needle aspiration for breast lumps, 4 developed pneumothorax as a complication. The incidence of this complication in our patients is 1:417. The clinical manifestations were immediate chest pain, shoulder pain and, occasionally, dyspnea that developed later. Observation is sufficient treatment in most patients, and rarely is drainage of the pneumothorax required.