Prevalence of Campylobacter species in meat, milk and other food commodities in Pakistan

A surveillance study was carried out to determine the prevalence of Campylobacter in meat, milk and other food commodities in Pakistan. Over a period of 3 years (January 2002-December 2004), a total of 1636 food samples of meat, milk and other food commodities were procured from three big cities of Pakistan (Faisalabad, Lahore and Islamabad) and were analysed. Among meat samples, the highest prevalence (48%) of Campylobacter was recorded in raw chicken meat followed by raw beef (10.9%) and raw mutton (5.1%). Among other food commodities, the highest prevalence was observed in vegetable/fruit salad (40.9%), sandwiches (32%), cheese (11%) and raw bulk milk samples (10.2%). The overall prevalence of Campylobacter was found to be 21.5%, out of which 70.6% were identified as Campylobacter (C.) jejuni and 29.4% as C. coli. The study reported that the prevalence of Campylobacter spp. was significantly higher in the food commodities, which included raw/undercooked ingredients.     

Algal ecology and composition in the Garmat ali river,Iraq

Planktonic, epipelic and epiphytic algae were studied monthly, during the period from January to December 1985 in the Garmat ali river, a naturally regulated river draining to the al-Hammar marsh, Iraq. Chlorophyll-a, primary productivity and water quality variables were also measured. High light penetration was noticed during the winter and spring. The water is oligohaline, alkaline and very hard. Reactive nitrate was found in higher concentrations than nitrite, and there were low concentrations of reactive phosphate. Two peaks were noticed for chlorophyll-a concentrations during spring and autumn, whereas only one occurred during autumn for primary productivity. The study area may be characterized as typically mesotrophic. A total of 154 species of algae was identified, including 65, 89, 98 and 77 species of plankton, epipelic and epiphytic algae on Potamogeton lucens and on Ceratophyllum demersum, respectively. Only 25 species were common to all the different communities. Diatoms were the dominant group in the epipelic and epiphytic algae during the study period, as well as in the plankton during spring and autumn. Seasonal variations were clear in the total cell numbers of algae from different habitats.     

The Therapeutic Potential of Exosomes in Soft Tissue Repair and Regeneration

Soft tissue defects are common following trauma and tumor extirpation. These injuries can result in poor functional recovery and lead to a diminished quality of life. The healing of skin and muscle is a complex process that, at present, leads to incomplete recovery and scarring. Regenerative medicine may offer the opportunity to improve the healing process and functional outcomes. Barriers to regenerative strategies have included cost, regulatory hurdles, and the need for cell-based therapies. In recent years, exosomes, or extracellular vesicles, have gained tremendous attention in the field of soft tissue repair and regeneration. These nanosized extracellular particles (30–140 nm) can break the cellular boundaries, as well as facilitate intracellular signal delivery in various regenerative physiologic and pathologic processes. Existing studies have established the potential of exosomes in regenerating tendons, skeletal muscles, and peripheral nerves through different mechanisms, including promoting myogenesis, increasing tenocyte differentiation and enhancing neurite outgrowth, and the proliferation of Schwann cells. These exosomes can be stored for immediate use in the operating room, and can be produced cost efficiently. In this article, we critically review the current advances of exosomes in soft tissue (tendons, skeletal muscles, and peripheral nerves) healing. Additionally, new directions for clinical applications in the future will be discussed.     

Temperature-Invariant Scaling for Compressible Turbulent Boundary Layers with Wall Heat Transfer

In a recent paper by Zhang et al. in 2012, a Mach number-invariant scaling was proposed to account for the effect of variation of free-stream Mach number in supersonic turbulent boundary layers. The present work focuses on the effect of variation of wall temperature with strong heating and cooling at the wall. Direct numerical simulation is used to study scaling and turbulence structure of a spatially evolving Mach 2 supersonic boundary layer at a friction Reynolds number of 500. A new scaling law is proposed to account for temperature-dependent fluid-property variations. This universal scaling appears superior to the existing models with the novelty that it applies not only for the mean-velocity profile but also extends to the turbulent transport, production, and dissipation terms in the budget of the turbulent kinetic energy.     

Highly-efficient technique for automatic segmentation of X-ray bone images based on fuzzy logic and an edge detection technique

Multidimensional Systems and Signal Processing - Development of medical image segmentation techniques has become one of the most important challenges in many applications that employ...     

Specific substitutions at amino acid 256 of the sarcoplasmic/endoplasmic reticulum Ca2+ transport ATPase mediate resistance to thapsigargin in thapsigargin-resistant hamster cells

High levels of resistance to thapsigargin (TG), a specific inhibitor of intracellular Ca2+ transport ATPases (SERCAs), can be developed in culture by stepwise exposure of mammalian cells to increasing concentrations of TG. We have identified, in two independently selected TG-resistant hamster cell lines of different lineages, mutant forms of SERCA. In the TG-resistant Chinese hamster lung fibroblast cell line DC-3F/TG, a T --> C change at nucleotide 766 introduces a Phe256 --> Leu alteration within the first cytosolic loop of the SERCA. In contrast, in the TG-resistant Syrian hamster smooth muscle cell line DDT/TG 4 microM, a T --> C change at nucleotide 767 introduces a Phe256 --> Ser mutation at that position. When these specific mutations are introduced into a wild-type full-length avian SERCA1 cDNA, transfection experiments reveal that Ca2+ transport function and ATP hydrolytic activity are not altered by such mutations. However, a 4-5-fold resistance to TG inhibition of Ca2+ transport function occurs upon the introduction of either the Phe256 --> Leu or the Phe256 --> Ser mutation into wild-type SERCA1. These specific mutations also render the hydrolytic activity of the ATPase resistant to inhibition by TG. Our results not only implicate amino acid 256 in TG-SERCA interactions, but also demonstrate that specific mutations within SERCA can mediate resistance to TG.     

Kinetics of catalytic esterification of terephthalic acid with methanol vapour

This paper describes the investigations carried out on the kinetics of the solid-gas reaction between terephthalic acid and methyl alcohol in presence of phosphoric acid as the catalyst. As phosphoric acid itself reacts with the alcohol forming methyl phosphates, the kinetic data obtained have been analysed taking this reaction into account. The rate of formation of monomethyl phosphate has been found to be proportional to the square of the mole fraction of the acid present at any instant. The activation energy involved in this reaction is 11.3 kcal/g mole. The data obtained on terephthalic acid esterification show that; (i) the conversion sharply increases with an increase in the catalyst concentration between 30–35 per cent (w/w), (ii) the ‘shrinking particle’ model, proposed by Levenspiel for solid-gas reactions, can be conveniently adapted to represent the reaction, and (iii) the temperature dependency of the reaction follows the Arrhenius law, with activation energy being 11.5 kcal/g mole.     

Amido-Amine-Based Cationic Gemini Surfactants: Thermal and Interfacial Properties and Interactions with Cationic Polyacrylamide

Experimental studies were conducted to investigate thermal and interfacial properties of two in‐house synthesized amido‐amine‐based cationic gemini surfactants namely: dodecanoic acid [3‐({4‐[(3‐dodecanoylamino‐propyl)‐dimethyl‐amino]‐butyl}‐dimethyl‐amino)‐propyl]‐amide dibromide ( 12‐4‐12 ) and dodecanoic acid [3‐({6‐[(3‐dodecanoylamino‐propyl)‐dimethyl‐amino]‐hexyl}‐dimethyl‐amino)‐propyl]‐amide dibromide ( 12‐6‐12 ). Thermogravimetric analysis showed the excellent thermal stability of surfactants and no structural degradation was observed at temperatures up to 250 °C. The long‐term thermal stability of the surfactants was investigated with the aid of spectroscopic techniques such as nuclear magnetic resonance (NMR (¹H and ¹³C) and Fourier transform infrared (FTIR) spectroscopy. Both surfactants were found to be thermally stable, and no changes in structure were observed after aging for 10 days at 90 °C. The interfacial tension of the surfactants was measured at three different temperatures (30, 60, and 80 °C), and the results showed a decrease in interfacial tension with increasing temperature and increasing spacer length of the surfactants. Rheological measurements were used to assess the interactions between the cationic gemini surfactant and cationic polyacrylamide. The addition of cationic surfactant reduced the viscosity and storage modulus of the polymer at low shear rate and frequency due to surfactant–polymer interactions and charge screening. The investigated surfactant–polymer system has great potential in high‐temperature carbonate reservoirs, where conventional anionic surfactants are not recommended due to high adsorption.     

Experimental estimation of the settling velocity and drag coefficient of the hollow cylindrical particles settling in non-Newtonian fluids in an annular channel

The drag coefficient data of particles settling in an annular channel is very much essential for designing different solid–fluid handling equipment, such as the fluidized bed. Experimental settling velocity, wall factor, and drag coefficient data of the hollow-cylinder particle are presented. Carboxymethyl cellulose solution has been used as the working fluid with a flow index of 0.64 ≤ n ≤ 0.91 and a consistency index of 0.31 ≤ K ≤ 1.81. The experimental results covered a wide diameter ratio range (0.14 ≤ d_eq/L ≤ 0.46), hollow cylinder inner to outer diameter ratio (0.2 ≤ d_i/d_o ≤0.8), and Reynolds number (0.05 ≤ Re ≤ 51 and 0.09 ≤ Re_∞ ≤ 55). d_eq, d_i, and d_o are the equivalent inner and outer diameters of the particle, L is the annular gap, and Re and Re_∞ are the Reynolds numbers in the presence and absence of the wall effect, respectively. The wall factor decreased, and the drag coefficient increased with d_eq/L and d_i/d_o ratios. The above parameters declined with the Reynolds number. The hollow cylinder experienced a lesser wall effect than the spherical particles settling in a non-annular channel. In some cases, the wall factor of the hollow cylinder is found to be equal to the spherical particles settling in an annular channel. The developed correlations have successfully predicted the drag coefficients of the hollow cylinder.