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91.
92.
Wenting Wu Peng Wang Xiaochun Huang Liwen Su Haixuan Lv Jiquan Gou Zhikui Cheng Lianlian Ma Wenjin Yu Zhengguo Liu 《International journal of molecular sciences》2022,23(13)
Soluble solids content (SSC) is an important quality trait of wax gourd, but reports about its regulatory genes are scarce. In this study, the SSC regulatory gene BhSSC2.1 in wax gourd was mined via quantitative trait locus (QTL) mapping based on high-density genetic mapping containing 12 linkage groups (LG) and bulked segregant analysis (BSA)-seq. QTL mapping and BSA-seq revealed for the first time that the SSC QTL (107.658–108.176 cM) of wax gourd was on Chr2 (LG2). The interpretable phenotypic variation rate and maximum LOD were 16.033% and 6.454, respectively. The QTL interval contained 13 genes. Real-time fluorescence quantitative expression analysis, functional annotation, and sequence analysis suggested that Bch02G016960, named BhSSC2.1, was a candidate regulatory gene of the SSC in wax gourd. Functional annotation of this gene showed that it codes for a NADP-dependent malic enzyme. According to BhSSC2.1 sequence variation, an InDel marker was developed for molecular marker-assisted breeding of wax gourd. This study will lay the foundation for future studies regarding breeding and understanding genetic mechanisms of wax gourd. 相似文献
93.
Die Su Yi Pei Li Liu Zhixiao Liu Junfang Liu Min Yang Jiaxing Wen Jing Dai Huiqiu Deng Guozhong Cao 《纳微快报(英文)》2021,(7):91-104
Wearable and portable mobile phones play a critical role in the market,and one of the key technologies is the flexible electrode with high spe-cific capacity an... 相似文献
94.
Haneul Lee Su Jin Kang Jimin Lee Kyong Hwa Park Won Jong Rhee 《International journal of molecular sciences》2022,23(13)
Prostate cancer (PCa) is the most commonly diagnosed malignancy among men in developed countries. The five-year survival rate for men diagnosed with early-stage PCa is approximately 100%, while it is less than 30% for castration-resistant PCa (CRPC). Currently, the detection of prostate-specific antigens as biomarkers for the prognosis of CRPC is criticized because of its low accuracy, high invasiveness, and high false-positive rate. Therefore, it is important to identify new biomarkers for prediction of CRPC progression. Extracellular vesicles (EVs) derived from tumors have been highlighted as potential markers for cancer diagnosis and prognosis. Specifically, urinary EVs directly reflect changes in the pathophysiological conditions of the urogenital system because it is exposed to prostatic secretions. Thus, detecting biomarkers in urinary EVs provides a promising approach for performing an accurate and non-invasive liquid biopsy for CPRC. In this study, we effectively isolated urinary EVs with low protein impurities using size-exclusion chromatography combined with ultrafiltration. After EV isolation and characterization, we evaluated the miRNAs in urinary EVs from healthy donors and patients with CRPC. The results indicated that miRNAs (miR-21-5p, miR-574-3p, and miR-6880-5p) could be used as potential biomarkers for the prognosis of CRPC. This analysis of urinary EVs contributes to the fast and convenient prognosis of diseases, including CRPC, in the clinical setting. 相似文献
95.
In the future smart transportation system, reliable vehicle-to-infrastructure(V2 I) communication is very important to ensure vehicle driving safety and to improve vehicle driving efficiency. In this paper, V2 I channel measurements at 5.92 GHz are conducted in typical urban and highway scenarios.The frequency and bandwidth of transmission, as well as the deployment of the RSU(roadside unit) and the OBU(on board unit), are selected by considering the recommendation proposed by 3 GPP TR 36.885. T... 相似文献
96.
电力电子系统集成对于中大功率电力电子装置而言是要实现其系统结构和部件的标准化.作者以多相同步电动机中压变频调速装置为研究背景,探索了复杂大电力电子装置中的系统集成问题.作者对大电力电子装置进行模块化研究,首先分析如何选择主电路的一级标准模块,如三电平逆变单元的一级标准模块为一相NPC三电平桥臂;然后又分析了如何划分系统的二级标准模块,如整流模块、逆变模块、励磁模块等;随后作者又以多相同步电动机调速系统为例对控制系统进行分层研究,设计了各控制管理层的任务及各层问的信息流通信方式.利用这些研究成果,作者设计、制作了模块化的同步电机调速装置,试验取得了成功. 相似文献
97.
Hsia-Wei Liu Wen-Ta Su Ching-Yi Liu Ching-Cheng Huang 《International journal of molecular sciences》2022,23(15)
A gelatin-based hydrogel scaffold with highly uniform pore size and biocompatibility was fabricated for cartilage tissue engineering using microfluidic 3D-foaming technology. Mainly, bubbles with different diameters, such as 100 μm and 160 μm, were produced by introducing an optimized nitrogen gas and gelatin solution at an optimized flow rate, and N2/gelatin bubbles were formed. Furthermore, a cross-linking agent (1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimide, EDC) was employed for the cross-linking reaction of the gelatin-based hydrogel scaffold with uniform bubbles, and then the interface between the close cells were broken by degassing. The pore uniformity of the gelatin-based hydrogel scaffolds was confirmed by use of a bright field microscope, conjugate focus microscope and scanning electron microscope. The in vitro degradation rate, mechanical properties, and swelling rate of gelatin-based hydrogel scaffolds with highly uniform pore size were studied. Rabbit knee cartilage was cultured, and its extracellular matrix content was analyzed. Histological analysis and immunofluorescence staining were employed to confirm the activity of the rabbit knee chondrocytes. The chondrocytes were seeded into the resulting 3D porous gelatin-based hydrogel scaffolds. The growth conditions of the chondrocyte culture on the resulting 3D porous gelatin-based hydrogel scaffolds were evaluated by MTT analysis, live/dead cell activity analysis, and extracellular matrix content analysis. Additionally, a dynamic culture of cartilage tissue was performed, and the expression of cartilage-specific proteins within the culture time was studied by immunofluorescence staining analysis. The gelatin-based hydrogel scaffold encouraged chondrocyte proliferation, promoting the expression of collagen type II, aggrecan, and sox9 while retaining the structural stability and durability of the cartilage after dynamic compression and promoting cartilage repair. 相似文献
98.
最少时间最小费用路问题的修改Dijkstra算法 总被引:2,自引:0,他引:2
针对同时带有顶点权和弧权的运输网络的最少时间最小费用路问题,首先将该网络转化为一般的只带弧权的运输网络,然后设计了求解该类问题的修改的“带前点标号的Dijkstra算法”,最后给出在物资公路运输中的一个实例。 相似文献
99.
100.