Length polymorphisms for intergenic spacer regions of 16S- 23S rDNA in members of the new hydrogen-producing bacteria

A method based on PCR amplification of the 16S rRNA gene (rDNA)-23S rDNA intergenic spacer regions (ISR) was developed for the identification of species within the novel group hydrogen-producing anaerobes. The sizes of the PCR products varied from 1264 to 398 bp. Strain of isolate Rennanqilyf 3 was characterized as having products of 1262,398,638,437 and 436 bp. The isolate Rennanqilyf 1 had product of 1264 bp. The isolate Rennanqilyf 13 had products of 1261,579 and 485 bp. Of the 3 species of the novel group hydrogen-producing anaerobes examined, no one was indistinguishable. Two environmental isolates were identified as hydrogen-producing bacteria, which were new species in present taxon. Rennanqilyf 3 could not be associated with any Clostridium sp. studied. Rennanqilyf 1 could be classified into Clostridium genus. The combination between 16S rDNA equencing and length polymorphisms of IRS in 16S-23S rDNA is a better method for determining species of the hydrogen-producing bacteria.     

La(Ⅲ)-IMP配合物的制备与催化合成乙酸异戊酯的研究

制备了稀土La(Ⅲ)与5-′次黄嘌呤核苷酸(5-′IMP)的配合物La(Ⅲ)-IMP,测定了其溶解性、IR、UV、电导率;并用作乙酸异戊酯合成的催化剂,探讨了催化剂用量、醇酸摩尔比、催化剂的重复使用对酯化反应的影响。结果表明La(Ⅲ)-IMP作为合成乙酸异戊酯的催化剂有较好的催化活性,醇酸摩尔比为21∶、催化剂用量为0.5g、反应温度为110℃、反应时间为2h时,乙酸异戊酯收率为60.8%。     

负载型多钒杂多酸催化剂在氧化脱硫反应中的应用

 使用两步法合成出H₁₂P₂Mo₁₂V₆O₆₂(HPA-6_2P)、H₁₄P₂Mo₁₀V₈O₆₂(HPA-8_2P)、H₁₅P₃Mo₁₈V₆O₈₄ (HPA-6_3P)和H₁₂P₃Mo₁₈V₇O₈₅(HPA-7_3P) Dawson 结构的含钒杂多酸化合物,并采用红外光谱、紫外光谱进行表征。以活性炭、二氧化硅为载体,浸渍以上化合物制备出负载型杂多酸催化剂。以二苯并噻吩(DBT)为模型化合物,考察了这类催化剂的氧化脱硫性能。结果表明,H₁₂P₃Mo₁₈V₇O₈₅(HPA-7_3P)/ C在90℃、5min的条件下,对二苯并噻吩(DBT)的氧化脱硫率可以达到73.2%,显示出优良的催化性能。     

微生物技术在油气田开发中的研究与应用进展

综述了微生物技术在油气勘探、渗透率调剖、提高采收率、清防蜡、原油破乳、浮油处理方面的应用,并对难点问题进行了分析,展望了微生物技术在油气田开发中的应用前景.