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51.
Otsuka H Hirano A Nagasaki Y Okano T Horiike Y Kataoka K 《Chembiochem : a European journal of chemical biology》2004,5(6):850-855
A two-dimensional microarray of ten thousand (100 x 100) hepatocyte heterospheroids, underlaid with endothelial cells, was successfully constructed with 100 microm spacing in an active area of 20 x 20 mm on microfabricated glass substrates that were coated with poly(ethylene glycol) brushes. Cocultivation of hepatocytes with endothelial cells was essential to stabilize hepatocyte viability and liver-specific functions, allowing us to obtain hepatocyte spheroids with a diameter of 100 microm, functioning as a miniaturized liver to secret albumin for at least one month. The most important feature of this study is that these substrates are defined to provide an unprecedented control of substrate properties for modulating cell behavior, employing both surface engineering and synthetic polymer chemistry. The spheroid array constructed here is highly useful as a platform of tissue and cell-based biosensors and detects a wide variety of clinically, pharmacologically, and toxicologically active compounds through a cellular physiological response. 相似文献
52.
AbstractA method for low-temperature drying with high drying rate was developed for heat-sensitive foods and agricultural products. A freezing pretreatment was combined with fluidized bed drying under reduced pressure. Cylindrical carrot samples were frozen and then placed without thawing in a fluidized bed of hygroscopic porous silica gel particles that adsorbed water from the sample during the drying process. The effects of the freezing pretreatment and hygroscopicity of fluidizing particles on the drying characteristics of carrots were examined. A higher drying rate was achieved when carrots samples were subjected to freezing pretreatment than without it. At 12?kPa, the volume change was smaller in carrots subjected to freezing pretreatment than in untreated samples. A larger amount of water was absorbed during rehydration by carrots dried at 12?kPa than at 101?kPa within 120?min. The properties of dried carrots were affected not only by freezing pretreatment but also by the pressure applied during the drying process. 相似文献
53.
54.
Poly(L-lactic acid) (PLLA) microfibers were obtained by a carbon dioxide (CO2) laser-thinning method. A laser-thinning apparatus used to continuously prepare microfibers was developed in our laboratory; it consisted of spools supplying and winding the fibers, a continuous-wave CO2-laser emitter, a system supplying the fibers, and a traverse. The laser-thinning apparatus produced PLLA microfibers in the range of 100-800 m min−1. The diameter of the microfibers decreased as the winding speed increased, and the birefringence increased as the winding speed increased. When microfibers, obtained through the laser irradiation (at a laser power of 8.0 W cm−2) of the original fiber supplied at 0.4 m min−1, were wound at 800 m min−1, they had a diameter of 1.37 μm and a birefringence of 24.1×10−3. The draw ratio calculated from the supplying and winding speeds was 2000×. The degree of crystal orientation increased with increasing the winding speed. Scanning electron microscopy showed that the microfibers obtained with the laser-thinning apparatus had smooth surfaces not roughened by laser ablation that were uniform in diameter. The PLLA microfiber, which was obtained under an optimum condition, had a Young's modulus of 5.8 GPa and tensile strength of 0.75 GPa. 相似文献
55.
Koji Makino 《Electrochimica acta》2005,51(5):961-965
The electrodes prepared by a sputtering method were evaluated as the cathodes for direct methanol fuel cells (DMFCs). Pt loading below 0.25 mg cm−2 achieved higher mass activities than that of 0.5 mg cm−2 prepared by the paste method, which was general conventional method. However, an increase in Pt loading reduced the catalyst activity for the oxygen reduction reaction (ORR). This result may suggest an increase in only electrochemically inactive Pt. Pt utilization efficiency can be found about ten times higher at Pt loading of 0.04 mg cm−2. Moreover, addition of Nafion to sputter-deposited Pt cathodes is found possible to improve the catalyst activity for the ORR, but the excess Nafion over the optimum condition reduces the active sites. 相似文献
56.
Toshiharu Makino Hiromitsu Kato Sung-Gi Ri Yigang Chen Hideyo Okushi 《Diamond and Related Materials》2005,14(11-12):1995
Electrical properties of homoepitaxial diamond p–n+ junction of boron (B)-doped p-type layer and phosphorus-doped n-type layer on Ib (111) diamond single crystal have been characterized. Current–voltage characteristics show a clear rectifying property with rectification ratio of over 105 at ± 10 V. From capacitance–voltage characteristics, it is found that a spatial distribution of space-charge density Ni of the p–n+ junction is not uniform and Ni at a middle region of the space-charge layer formed at zero bias voltage is higher than that of other region of the space-charge layer. This peculiar characteristic can be explained by superposition of two effects; one is the deep dopant effect due to B atoms in the p-type layer, which makes to reduce Ni at around the edge of the space-charge layer formed at zero bias voltage. The other is the compensation of B acceptors by impurity atoms diffusing during the p–n+ interface and incorporating during the growth of p-type layer, which makes to reduce Ni at the vicinity of the p–n+ interface. 相似文献
57.
Akihiro Shimamura Manabu Fukushima Mikinori Hotta Tatsuki Ohji Naoki Kondo 《Journal of the American Ceramic Society》2016,99(2):440-444
A novel fabrication route to make macroporous silicon carbide (SiC) has been proposed in this study. The route is composed of the following two steps: the fabrication of porous α‐SiC/novolac‐type phenolic composite using hexamethylenetetramine (HMT) as a curing/blowing agent for the novolac monomer and a conventional reaction‐bonded (RB) sintering of the composite. The α‐SiC/novolac‐type phenolic composite was carbonized at 800°C for 2 h in N2 gas and then reacted with the molten silicon at 1450°C for 30 min under vacuum, resulting in the macroporous RB‐SiC with an open porosity of 48% and relatively large pore size of ~110 μm. The compressive strength of the macroporous RB‐SiC was 113 MPa, which is relatively high compared to those reported for macroporous SiC of equivalent porosities and pore sizes. 相似文献
58.
Kaname Uno Shohei Iyoshi Masato Yoshihara Kazuhisa Kitami Kazumasa Mogi Hiroki Fujimoto Mai Sugiyama Yoshihiro Koya Yoshihiko Yamakita Akihiro Nawa Tomohiro Kanayama Hiroyuki Tomita Atsushi Enomoto Hiroaki Kajiyama 《International journal of molecular sciences》2022,23(8)
Epithelial ovarian cancer (EOC) is the most lethal gynecologic malignancy and has a unique metastatic route using ascites, known as the transcoelomic root. However, studies on ascites and contained cellular components have not yet been sufficiently clarified. In this review, we focus on the significance of accumulating ascites, contained EOC cells in the form of spheroids, and interaction with non-malignant host cells. To become resistant against anoikis, EOC cells form spheroids in ascites, where epithelial-to-mesenchymal transition stimulated by transforming growth factor-β can be a key pathway. As spheroids form, EOC cells are also gaining the ability to attach and invade the peritoneum to induce intraperitoneal metastasis, as well as resistance to conventional chemotherapy. Recently, accumulating evidence suggests that EOC spheroids in ascites are composed of not only cancer cells, but also non-malignant cells existing with higher abundance than EOC cells in ascites, including macrophages, mesothelial cells, and lymphocytes. Moreover, hetero-cellular spheroids are demonstrated to form more aggregated spheroids and have higher adhesion ability for the mesothelial layer. To improve the poor prognosis, we need to elucidate the mechanisms of spheroid formation and interactions with non-malignant cells in ascites that are a unique tumor microenvironment for EOC. 相似文献
59.
60.
Hiromi Nabeshi Tomoaki Yoshikawa Akihiro Arimori Tokuyuki Yoshida Saeko Tochigi Toshiro Hirai Takanori Akase Kazuya Nagano Yasuhiro Abe Haruhiko Kamada Shin-ichi Tsunoda Norio Itoh Yasuo Yoshioka Yasuo Tsutsumi 《Nanoscale research letters》2011,6(1):1-6
In this study, complexes composed of poly-l-tyrosine (pLT) and single-walled carbon nanotubes (SWCNTs) were produced and the dispersibility of the pLT/SWCNT complexes in water by measuring the ζ potential of the complexes and the turbidity of the solution were investigated. It is found that the absolute value of the ζ potential of the pLT/SWCNT complexes is as high as that of SWCNTs modified with double-stranded DNA (dsDNA) and that the complexes remain stably dispersed in the water at least for two weeks. Thermogravimetry analysis (TGA) and visualization of the surface structures of pLT/SWCNT complexes using an atomic force microscope (AFM) were also carried out. 相似文献