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101.
Alexandre J. Kabla 《Journal of the Royal Society Interface》2012,9(77):3268-3278
A number of biological processes, such as embryo development, cancer metastasis or wound healing, rely on cells moving in concert. The mechanisms leading to the emergence of coordinated motion remain however largely unexplored. Although biomolecular signalling is known to be involved in most occurrences of collective migration, the role of physical and mechanical interactions has only been recently investigated. In this study, a versatile framework for cell motility is implemented in silico in order to study the minimal requirements for the coordination of a group of epithelial cells. We find that cell motility and cell–cell mechanical interactions are sufficient to generate a broad array of behaviours commonly observed in vitro and in vivo. Cell streaming, sheet migration and susceptibility to leader cells are examples of behaviours spontaneously emerging from these simple assumptions, which might explain why collective effects are so ubiquitous in nature. The size of the population and its confinement appear, in particular, to play an important role in the coordination process. In all cases, the complex response of the population can be predicted from the knowledge of the correlation length of the velocity field measured in the bulk of the epithelial layer. This analysis provides also new insights into cancer metastasis and cell sorting, suggesting, in particular, that collective invasion might result from an emerging coordination in a system where single cells are mechanically unable to invade. 相似文献
102.
Mohammad Golbabaee Alexandre Alahi Pierre Vandergheynst 《Journal of Mathematical Imaging and Vision》2014,48(1):160-175
Detecting and tracking people in scenes monitored by cameras is an important step in many application scenarios such as surveillance, urban planning or behavioral studies to name a few. The amount of data produced by camera feeds is so large that it is also vital that these steps be performed with the utmost computational efficiency and often even real-time. We propose SCOOP, a novel algorithm that reliably localizes people in camera feeds, using only the output of a simple background removal technique. SCOOP can handle a single or many video feeds. At the heart of our technique there is a sparse model for binary motion detection maps that we solve with a novel greedy algorithm based on set covering. We study the convergence and performance of the algorithm under various degradation models such as noisy observations and crowded environments, and we provide mathematical and experimental evidence of both its efficiency and robustness using standard datasets. This clearly shows that SCOOP is a viable alternative to existing state-of-the-art people localization algorithms, with the marked advantage of real-time computations. 相似文献
103.
Although traditional approaches to code profiling help locate performance bottlenecks, they offer only limited support for removing these bottlenecks. The main reason is the lack of detailed visual runtime information to identify and eliminate computation redundancy. We provide three profiling blueprints that help identify and remove performance bottlenecks. The structural distribution blueprint graphically represents the CPU consumption share for each method and class of an application. The behavioral distribution blueprint depicts the distribution of CPU consumption along method invocations and hints at method candidates for caching optimizations. The behavioral evolution blueprint compares profiles of different versions of a software system and highlights performance‐critical changes in the system. These three blueprints helped us to significantly optimize Mondrian, an open source visualization engine. Our implementation is freely available for the Pharo development environment and has been evaluated in a number of different scenarios. Copyright © 2011 John Wiley & Sons, Ltd. 相似文献
104.
Valdivino Alexandre de Santiago Júnior Nandamudi Lankalapalli Vijaykumar 《Software Quality Journal》2012,20(1):77-143
Natural Language (NL) deliverables suffer from ambiguity, poor understandability, incompleteness, and inconsistency. Howewer,
NL is straightforward and stakeholders are familiar with it to produce their software requirements documents. This paper presents
a methodology, SOLIMVA, which aims at model-based test case generation considering NL requirements deliverables. The methodology
is supported by a tool that makes it possible to automatically translate NL requirements into Statechart models. Once the
Statecharts are derived, another tool, GTSC, is used to generate the test cases. SOLIMVA uses combinatorial designs to identify
scenarios for system and acceptance testing, and it requires that a test designer defines the application domain by means
of a dictionary. Within the dictionary there is a Semantic Translation Model in which, among other features, a word sense disambiguation method helps in the translation process. Using as a case study
a space application software product, we compared SOLIMVA with a previous manual approach developed by an expert under two
aspects: test objectives coverage and characteristics of the Executable Test Cases. In the first aspect, the SOLIMVA methodology
not only covered the test objectives associated to the expert’s scenarios but also proposed a better strategy with test objectives
clearly separated according to the directives of combinatorial designs. The Executable Test Cases derived in accordance with
the SOLIMVA methodology not only possessed similar characteristics with the expert’s Executable Test Cases but also predicted
behaviors that did not exist in the expert’s strategy. The key benefits from applying the SOLIMVA methodology/tool within
a Verification and Validation process are the ease of use and, at the same time, the support of a formal method consequently
leading to a potential acceptance of the methodology in complex software projects. 相似文献
105.
Peptidases occupy a central position in the enzyme market because of their importance in many areas, such as for physiological processes, foods, and detergents, as well as in the pharmaceutical, leather, and biotechnology industries. Microbial production is among the major sources of peptidases because it presents many advantages when compared with other methods. In this study, the metallopeptidases produced by the fungus Eupenicillium javanicum under a solid-state fermentation bioprocess were spray-dried. The enzymatic extract was dried using drying adjuvants, and optimal conditions for preserving enzymatic activity were studied following a Box-Behnken experimental design. The spray process factors studied were the air-drying temperature, enzyme feed flow rate, and the proportion of enzyme/additive. The responses analyzed were the dry extract yield and enzymatic activity after spray drying. Additionally, the stability of the dry extracts was assessed during 180 days at 4°C and 25°C. The results revealed extract yields of up to 66.12% and good enzymatic activity for intermediate values of temperature and adjuvant proportions. Furthermore, the dried enzymatic extracts showed potential for future commercial applications because of their stability at 25°C for 180 days. 相似文献
106.
Alexandre Ruellan Violette Ducruet Audrey Gratia Lidia Saelices Jimenez Alain Guinault Cyrille Sollogoub Guillaume Chollet Sandra Domenek 《Polymer International》2016,65(6):683-690
Polylactide (PLA) is the most used biodegradable and biobased food packaging polymer for rigid containers and films. However, its low ductility is a hurdle for increasing its applications in flexible food packaging. A solution is the use of additives. Palm oil deodorizer distillate (PODC) is revealed to be an excellent additive promoting PLA ductility. PODC is a by‐product of vegetable oil refining, which is available in stable quality and in sufficient amounts. Amorphous PLA/PODC blends had an elongation at break of around 130% and that of semi‐crystalline blends was still around 55% compared to the initial 5% of neat PLA. At the same time the PLA rigidity and high glass transition temperatures were kept. PODC was also a very efficient processing aid, allowing for film blow extrusion. The blends were stable in properties during six months without exudation. They complied with legal norms of Food Contact Materials (EU 10/2011) and induced no sensorial alteration of packed food. Therefore PODC is a very interesting alternative to common plasticizers for the production of flexible PLA packaging films. © 2016 Society of Chemical Industry 相似文献
107.
Alexandre Antunes Ribeiro Rodrigo Fernando Costa Marques Antônio Carlos Guastaldi João Sinézio de Carvalho Campos 《Journal of Materials Science》2009,44(15):4056-4061
Many techniques have been used to coat metallic substrate with bioceramics. The aim of this study was to study the physical-chemical
characteristics of polyvinylidene fluoride (α-PVDF)/hydroxyapatite (HA) composite coating, obtained by casting method, on
commercially pure titanium (α-CP Ti) substrate surface modified by laser beam irradiation. The preparation of coating was
done for mixing α-PVDF pellets shape dissolved in dimethylacetamide (DMA) with HA/DMA emulsion. The mixture was poured onto
the α-CP Ti sample and left to dry in an oven. CP Ti plates were coated with α-PVDF/HA composite film, in proportions of 100/00
and 60/40 in weight, and characterized by particle size analysis, scanning electron microscopy, energy dispersive spectroscopy
(EDS), X-ray diffractometry, thickness measurement and contact angle. Uniform coating with a small thickness variation along
the coated surface was successfully obtained. 相似文献
108.
We present a novel comprehensive optimization model for designing reconfigurable machining lines. Due to the proposed fine mathematical modelling, it is possible to optimize simultaneously the whole set of machines and machining modules as well as their cutting parameters, their configuration that will be used for processing of each part and part position at each machine. The experimental results show that the proposed optimization approach substantially outperforms the existing heuristic design method and therefore it can be used by the designers in order to reduce the total system cost and improve the efficiency of reconfigurable machining lines. 相似文献
109.
Impact of oxygen transport properties on the kinetic modeling of polypropylene thermal oxidation. II. Effect of oxygen diffusivity
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Alexandre François‐Heude Emmanuel Richaud Alain Guinault Eric Desnoux Xavier Colin 《应用聚合物科学杂志》2015,132(6)
The kinetic model, established in a previous article (François‐Heude et al., J. Appl. Polym. Sci., in press) to predict the homogeneous oxidation in iPP films typically thinner than 100 µm, is now extended to simulate the oxidation profiles in thicker plates by coupling the oxygen diffusion and its consumption by the chemical reactions. In this perspective, oxygen transport properties (namely oxygen solubility, diffusivity, and permeability) are measured by permeametry on a reference iPP. These values are compared with an exhaustive compilation of literature data to evaluate their variability among the whole iPP family, which one has been reasonably ascribed to initial differences in polymer morphology, but also to evaluate their consistency, especially their temperature dependence between 20 and 140°C. Failing to simulate oxidation profiles, the kinetic model is then used as an inverse resolution method for estimating more satisfactory values of oxygen transport properties. It is thus evidenced that the crystallinity changes induced by thermal oxidation largely explains the dramatic decrease in oxygen penetration toward the sample core just after the induction period. A strategy aimed for introducing the relationship between the polymer crystalline morphology and oxygen transport properties into the kinetic model is given in the graphical abstract, although the effect of polymer polarity remains to be established prior to this implementation. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132, 41562. 相似文献
110.
Oxidative Stability and Changes in Chemical Composition of Extra Virgin Olive Oils After Short‐Term Deep‐Frying of French Fries
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Emília Akil Vanessa Naciuk Castelo‐Branco André Mesquita Magalhães Costa Ana Lúcia do Amaral Vendramini Verônica Calado Alexandre Guedes Torres 《Journal of the American Oil Chemists' Society》2015,92(3):409-421
We aimed at investigating oxidative stability and changes in fatty acid and tocopherol composition of extra virgin olive oil (EVOO) in comparison with refined seed oils during short‐term deep‐frying of French fries, and changes in the composition of the French fries deep‐fried in EVOO. EVOO samples from Spain, Brazil, and Portugal, and refined seed oils of soybean and sunflower were studied. Oil samples were used for deep‐frying of French fries at 180 °C, for up to 75 min of successive frying. Tocopherol and fatty acid composition were determined in fresh and spent vegetable oils. Tocopherol, fatty acid, and volatile composition (by SPME–GC–MS) were also determined in French fries deep‐fried in EVOO. Oil oxidation was monitored by peroxide, acid, and p‐anisidine values, and by Rancimat after deep‐frying. Differential scanning calorimetry (DSC) analysis was used as a proxy of the quality of the spent oils. EVOOs presented the lowest degree of oleic and linoleic acids losses, low formation of free fatty acids and carbonyl compounds, and were highly stable after deep‐frying. In addition, oleic acid, tocopherols, and flavor compounds were transferred from EVOO into the French fries. In conclusion, EVOOs were more stable than refined seed oils during short‐term deep‐frying of French fries and also contributed to enhance the nutritional value, and possibly improve the flavor, of the fries prepared in EVOO. 相似文献