Limits on a Common Approximation for Layered Consolidation Analysis

A widely used procedure for solving the problem of vertical consolidation of layers with different properties is to transform the thicknesses of the layers in proportion to the square root of their coefficients of consolidation. However, the Gray–Barber closed-form solution shows that this procedure is not correct except under a restrictive set of conditions. The transformation method should not be used. When solutions are needed for layered systems, the Gary–Barber solution or numerical methods should be employed.     

Seasonal changes and temperature-dependent accumulation of polycyclic aromatic hydrocarbons in high-altitude soils

Polycyclic Aromatic Hydrocarbons (PAHs) are a major group of pollutants whose occurrence in the environment is mainly of anthropogenic origin. In this paper, we examine the effect of topographical slope exposure on PAH contamination and seasonal change in PAH concentrations in soils. We collected soil samples on three dates in 2007 (early May, end of July and beginning of November) from south- and north-facing aspects at 1900 m a.s.l. in the central Italian Alps. We found greater PAH contamination in soils from a north-facing slope than in those from a south-facing slope at all seasons. We calculated North-South Enrichment Factors as the ratio between the concentrations measured in soils from northern and southern aspects. These ratios ranged from 1.4 to 1.9 for lighter PAHs (from 2 to 4 rings). These values are consistent with theoretical calculations based on temperature-specific octanol-air partition coefficients (predicted North-South Enrichment Factors range from 1.6 to 2.0). For heavier PAHs (from 5 to 6 rings), smaller differences were observed between soils from northern and southern aspects, due to the gas/particle distribution of these compounds. We also found consistent differences in normalised PAH concentrations across the three sampling periods. The majority of compounds showed a significant decreasing trend from the beginning of May to the end of July, due to the annual cycles of physical processes (deposition vs. volatilisation) and biological processes (uptake and/or biotransformation). Only a few compounds showed different trends, presumably due to season-specific local emission sources.     

Transformation Methods for the Numerical Integration of Three-Dimensional Singular Functions

The implementations of the eXtended Finite Element Method and the Boundary Element Method need to face the challenge of integrating singular functions. Since standard quadrature techniques usually produce inaccurate results, a number of specific algorithms have been developed to address this problem. We present a general framework for the systematic formulation of the three-dimensional case. The classical cubic transformation is also considered, including an analytical optimization of its parameters for improved practical efficiency.     

EXPLORATION AND EVALUATION OF THE ZUATA AREA, ORINOCO OIL BELT, VENEZUELA

Exploration activity in the Zuata area within the Orinoco Oil Belt in Venezuela has included the drilling of 161 wells and the recording of 3,000 km of seismic lines. Based on these results, a productive area of 9,200 sq. km has been defined, containing 79.3 B cu.m. (499 B brl)? of crude oil in-place, with densities ranging from 1.021 to 0.934 g/cu.cm (7–20 °API). The principal productive sands are of the Tertiary Officina Formation, deposited during a transgression which occurred in the Miocene. A distinctive feature of the productive formation is its progressive onlap against older rocks (Cretaceous, Paleozoic or Pre-Cambrian) in a southerly direction which, together with overlying shales, provide the trapping mechanism for the oil which migrated from the north. The productive sands were deposited in a fluvial to coastal plain with deltaic developments. The sands are unconsolidated and fine- to very coarse-grained, with an average porosity of 34% and a permeability of 7μm²(7D). The structure is a monocline dipping 0.5° to 2° to the NE, and the trapping mechanism is principally stratigraphic. In the 134 producers, 300 tests were completed which resulted in an average production of 25 cu.m/d (160 b/d) per well by beam pumping without dilution or heat. Subsequently, a steam cycle of 5,000 metric tons was injected into 16 wells; production increased to some 200 cu.m/d (1,250 b/d) per well. For future development, a Priority Area has been selected: this Area covers 3,500 sq kin, and contains 40.5 B cu.m (255 B brl) of crude oil with a density range of 1.014 to 0.993 g/cu.cm (8–11°API). The oil is at an average depth of 610 m, and the average net oil-sand thickness is 60 m. The oil-sand is generally divided into three zones separated by shales, and the gross productive thickness is some 110 m. Similar to Maraven ‘s experience in the oilfields of the Bolivar Coast in Western Venezuela, it may be expected that reservoir compaction will occur, and will be efficiently activated by steam-soak or “huff and puff’. Based on core compressibility measurements, a recovery factor from compaction drive and solution gas treatments could reach 12 % of the oil in-place. Under this premise, the Priority Area could have a potential recovery of 4.9 B cu. m. (30.6 B brl), which could support a production of 160,000 cu.m/d(1MM b/d) for 100 years. Any follow-up steam drive can be assumed to recover at least an additional 10% of the oil in-place. A Conceptual Development Plan based on clusters, inclined wells (due to the shallow depth) and steam soak has been proposed, and will be validated by a pilot test consisting of a seven-well cluster.     

3D TEM Tomography of Templated Bilayer Films of Block Copolymers

Transmission electron microscope (TEM) tomography was used to visualize the morphology and 3D connectivity of a lithographically templated, self‐assembled bilayer film of cylinder‐forming 45.5 kg/mol polystyrene‐block‐polydimethylsiloxane. The structure, formed after a 5 min solvothermal anneal, was imaged with a resolution of ≈3 nm in 3D, enabling a comparison between measurement and self‐consistent mean‐field theory (SCFT) calculations. Images of etched and unetched samples showed that etching collapsed the PDMS microdomain structure and reduced the template dimensions. In addition to the general comparison between modeled and measured dimensions, the tomography revealed connections between the orthogonal layers of cylinders at their crossing points. Comparison with the SCFT model, even under solvothermal annealing conditions, shows that it is helpful in understanding the detailed nanoscale structure of features created by directed self‐assembly (DSA), which is essential in developing nanomanufacturing processes based on DSA.     

Rapid Detection of Wheat Blast Pathogen Magnaporthe oryzae Triticum Pathotype Using Genome-Specific Primers and Cas12a-mediated Technology

Wheat blast, caused by the fungus Magnaporthe oryzae Triticum (MoT) pathotype, is a devastating disease persistent in South America and Bangladesh. Since MoT generally fails to cause visual symptoms in wheat until the heading stage when the infection would have advanced, disease control by fungicide application solely based on the detection of visual symptoms is ineffective. To develop an accurate and sensitive method to detect MoT at the seedling and vegetative stages for disease control, we sequenced the genomes of two MoT isolates from Brazil and identified two DNA fragments, MoT-6098 and MoT-6099, that are present in the MoT genome but not in the genome of the rice-infecting Magnaporthe oryzae Oryzae (MoO) pathotype. Using polymerase chain reaction (PCR), we confirmed the specificity of the two markers in 53 MoT and MoO isolates from South America and Bangladesh. To test the efficiency of the two markers, we first established a loop-mediated isothermal amplification (LAMP) method to detect MoT at isothermal conditions, without the use of a PCR machine. Following this, we used the Cas12a protein and guide RNAs (gRNAs) to target the MoT-6098 and MoT-6099 sequences. The activated Cas12a showed indiscriminate single-stranded deoxyribonuclease (ssDNase) activity. We then combined target-dependent Cas12a ssDNase activation with recombinase polymerase amplification (RPA) and nucleic acid lateral flow immunoassay (NALFIA) to develop a method that accurately, sensitively, and cost-effectively detects MoT-specific DNA sequences in infected wheat plants. This novel technique can be easily adapted for the rapid detection of wheat blast and other important plant diseases in the field.     

Solutions to a Radical Problem: Overview of Current and Future Treatment Strategies in Leber’s Hereditary Opic Neuropathy

Leber’s Hereditary Optic Neuropathy (LHON) is the most common primary mitochondrial DNA disorder. It is characterized by bilateral severe central subacute vision loss due to specific loss of Retinal Ganglion Cells and their axons. Historically, treatment options have been quite limited, but ongoing clinical trials show promise, with significant advances being made in the testing of free radical scavengers and gene therapy. In this review, we summarize management strategies and rational of treatment based on current insights from molecular research. This includes preventative recommendations for unaffected genetic carriers, current medical and supportive treatments for those affected, and emerging evidence for future potential therapeutics.     

Microbiota Composition in Diverticular Disease: Implications for Therapy

Gut microbiota (GM) composition and its imbalance are crucial in the pathogenesis of several diseases, mainly those affecting the gastrointestinal tract. Colon diverticulosis and its clinical manifestations (diverticular disease, DD) are among the most common digestive disorders in developed countries. In recent literature, the role of GM imbalance in the onset of the different manifestations within the clinical spectrum of DD has been highlighted. This narrative review aims to summarize and critically analyze the current knowledge on GM dysbiosis in diverticulosis and DD by comparing the available data with those found in inflammatory bowel disease (IBD). The rationale for using probiotics to rebalance dysbiosis in DD is also discussed.