A Multifunctional and Fast-Response Lysosome-Targetable Fluorescent Probe for Monitoring pH and Isoxaflutole

A new chemosensor, namely N-(2-morpholinoethyl)acetamide-4-morpholine-1,8-naphthimide (MMN), was designed and synthesized through an amidation reaction. MMN was fabricated as a multifunctional fluorescent probe for monitoring pH and isoxaflutole. MMN exhibited excellent stability in MeCN/H₂O (v/v, 9/1), with an obvious “off–on” fluorescence response toward pH changes due to intramolecular charge transfer (ICT), where the linear response ranges of MMN in the weakly acidic system were from 4.2 to 5.0 and from 5.0 to 6.0 with apparent pK_a = 4.62 ± 0.02 and 5.43 ± 0.02. Based on morpholine as the lysosome targetable unit, MMN could selectively locate lysosomes in live cells. MMN also successfully detected the presence of H⁺ in test papers. Finally, MMN could specifically recognize isoxaflutole at a detection limit of 0.88 μM. A possible sensing mechanism was identified based on density function theory calculations. These results indicate that MMN could be a superior potential chemosensor for detecting pH and isoxaflutole selectively and sensitively and could be used in real sample detection.     

矿井提升机下坠现象的解决措施

提出了矿井提升机下坠现象的解决措施,就此作了理论分析,并用计算机仿真出了速度跟随曲线。     

葡萄酒中铁含量测定的影响因素

葡萄酒中含有铁,而铁又是造成葡萄酒变质的重要因素,因此铁的测定就显得格外重要.文中采用邻啡罗啉比色法测定葡萄酒中铁含量,通过实际操作,探讨了不同波长的吸光度、显色时间、显色剂用量、PH值等对实验结果的影响.     

QSAR,Docking, and Molecular Dynamics Simulation Studies of Sigmacidins as Antimicrobials against Streptococci

Streptococci are a family of bacterial species significantly affecting human health. In addition, environmental Streptococci represent one of the major causes of diverse livestock diseases. Due to antimicrobial resistance, there is an urgent need for novel antimicrobial agent discovery against Streptococci. We discovered a class of benzoic acid derivatives named sigmacidins inhibiting the bacterial RNA polymerase-σ factor interaction and demonstrating excellent antimicrobial activity against Streptococci. In this work, a combinational computer approach was applied to gain insight into the structural basis and mechanism of action of sigmacidins as antimicrobials against Streptococcus pneumoniae. Both two- and three-dimensional quantitative structure-active relationships (2D and 3D QSAR) of sigmacidins displayed good predictive ability. Moreover, molecular docking and molecular dynamics simulation studies disclosed possible contacts between the inhibitors and the protein. The results obtained in this study provided understanding and new directions to the further optimizations of sigmacidins as novel antimicrobials.     

我国酒母培养技术的变迁

我国与西方的酿酒方法截然不同，以曲酿酒，其中酒母的培养技术大致分为浸曲法（汉唐时期）、浓缩酸浆酒母法（唐宋时期）、自然培养酒母法（宋代）、淋饭酒母法和速酿酒母法（现代）。（庞晓）     

一种改进的气动弹性时域推进方法

针对气动弹性控制方程,结构项采用标准龙格库塔方法推进,广义气动力项采用时间上的插值技术,发展了一种在每一时间步内只用计算一次非定常气动力的近似四阶龙格库塔推进方法。通过计算非线性Bernoulli方程和某一超音速翼面的气动弹性响应,证明该方法的时间准确度远高于采用气动力冻结技术的四阶龙格库塔方法。其准确度与标准龙格库塔方法相差不大,而其调用非定常气动力求解器的次数仅为其四分之一,大大提高了气动弹性数值模拟的效率。对于一般的气动弹性响应而言,该时间推进方法只用保证每个周期内的时间步在20～30个左右就能满足较高的准确度要求。