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91.
92.
Beatriz A. Rodas-Junco Graciela E. Racagni-Di-Palma Michel Canul-Chan Javier Usorach S. M. Teresa Hernndez-Sotomayor 《International journal of molecular sciences》2021,22(5)
Plants are subject to different types of stress, which consequently affect their growth and development. They have developed mechanisms for recognizing and processing an extracellular signal. Second messengers are transient molecules that modulate the physiological responses in plant cells under stress conditions. In this sense, it has been shown in various plant models that membrane lipids are substrates for the generation of second lipid messengers such as phosphoinositide, phosphatidic acid, sphingolipids, and lysophospholipids. In recent years, research on lipid second messengers has been moving toward using genetic and molecular approaches to reveal the molecular setting in which these molecules act in response to osmotic stress. In this sense, these studies have established that second messengers can transiently recruit target proteins to the membrane and, therefore, affect protein conformation, activity, and gene expression. This review summarizes recent advances in responses related to the link between lipid second messengers and osmotic stress in plant cells. 相似文献
93.
94.
Beatriz García-Fontana Sonia Morales-Santana Victoria Longobardo Rebeca Reyes-García Pedro Rozas-Moreno José Antonio García-Salcedo Manuel Mu?oz-Torres 《International journal of molecular sciences》2015,16(5):9469-9483
Type 2 diabetes mellitus patients are at significant risk of cardiovascular disease, however, the pathophysiology of these complications is complex and incompletely known in this population. The aim of this study was to compare the serum proteome of patients with type 2 diabetes mellitus presenting or not presenting cardiovascular disease with non-diabetic subjects to find essential proteins related to these cardiovascular complications. This cross-sectional study compares the serum proteome by a combination of protein depletion with 2D-DIGE (2-dimension Difference Gel Electrophoresis) methodology. The proteins differentially expressed were identified by MALDI TOF/TOF (Matrix-assisted laser desorption/ionization and Time-Of-Flight ion detector) or LC-MS/MS (Liquid Chromatography coupled to Mass-Mass Spectrometry). Type 2 diabetes mellitus patients with cardiovascular disease showed higher expression of plasma retinol binding protein and glutathione peroxidase-3 compared to those without cardiovascular disease and non-diabetic controls. These results show that proteins related to the inflammatory and redox state appear to play an important role in the pathogenesis of the cardiovascular disease in the type 2 diabetes mellitus patients. 相似文献
95.
On May 4th,2006,the international publication of a patentapplicationtitled“alloyed zinc powders for alkaline batteries withhigh Pyknometer density”WO2006/045470 A1[1],appeared·Theintention of this investigation was to see whether it is possible torepro… 相似文献
96.
Beatriz Prieto Fernando Tricas Juan J. Merelo Antonio Mora Alberto Prieto 《Journal of Network and Computer Applications》2008,31(4):677-698
Weblogs are dynamic websites updated via easy-to-use content management systems and organized as a set of chronologically ordered stories, frequently built around a link or including links to other weblogs. Since they are managed by individuals, their links tend to mirror or, in some cases, establish new types of social relations, thereby creating a social network. Studying the evolution of this network allows the discovery of emerging social structures and their growth trends. In this paper, we demonstrate the advantages of using the self-organizing maps (SOM) to visualize the evolution of a social network formed by a set of blogs, from their beginning to their current state. By observing the position a weblog is mapped to, it is easy to see what communities it belongs to nowadays, and how and when it became a part of those communities. The proposed procedure gives some insight on how communities are formed and have evolved. In this study, we apply this method to Blogalia, a blog-hosting site from which we have obtained a complete set of data and, by using SOM projections, we have drawn some conclusions on what drives the evolution of its implicit social network. 相似文献
97.
Spectrophotometric data followed by a suitable treatment of chemometric analysis were used for the simultaneous determination of monosodium glutamate (MSG), guanosine 5′-monophosphate (GMP) and inosine 5′-monophosphate (IMP) in stock cube samples, without any previous extraction step. By this way, the overlapping of the absorption spectra was resolved using a PLS-1 model. The concentration for experimental calibration matrix were varied between 5.03–34.2 μg mL−1 for IMP and GMP, and 448–1399 μg mL−1 for MSG. The relative errors of prediction (REPCV %) were 1.8, 2.8 and 3.1 for IMP, GMP and MSG, respectively. 相似文献
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99.
Sara C Cunha José O Fernandes M Beatriz P P Oliveira 《Food Additives & Contaminants》2007,24(2):156-164
Matrix solid-phase dispersion (MSPD) methodology has been developed to extract fenthion and its metabolites from olives and olive oils, and the technique compared with conventional liquid-liquid extraction (LLE). The method was applied to olives and olive oil samples obtained from olive groves treated with fenthion. Pesticide residues were analysed by gas chromatography (GC) using a nitrogen-phosphorus detector. Both extraction methods gave a linear response over the concentration range assayed (0.03-1 mg kg-1 for MSPD and 0.1-1 mg kg-1 for LLE). Recoveries and RSD (n = 6) values in olives were 85-112% and 2-6% for MSPD, and 68-108% and 4-16% for LLE, respectively. In the case of olive oil, recoveries and RSD (n = 6) values were 67-98% and 5-11% for MSPD, and 63-115% and 6-14% for LLE, respectively. When compared to LLE, the newly developed MSPD method was twice as sensitive and required 10 times less sample weight. 相似文献
100.
Miguel Angelo Faria Eugénia Nunes Maria Beatriz P. P. Oliveira 《European Food Research and Technology》2008,227(3):845-850
Varietal wines are defined as made primarily from a single variety of grape, which is identified on the label. However the
inclusion of other varieties in their production is permitted under defined percentages. The fact that different grape varieties/varietal
wines present far different economical values can attract fraudulent practices. Thus the development of methods, which allow
the quantification of those varieties, is of crucial importance. The first approach to the method for the relative quantification
of must containing two varieties based on microsatellite DNA analysis is presented. Quantification was achieved by densitometry
measurements of the amplification products of the pooled varieties, obtained with loci VVMD6 and VVMD7, after their separation and staining on polyacrilamide gels. We have found that a good correlation between
the proportion of each variety in must and the signal intensity of the alleles are dependent on the variety (varietal effect)
and the microsatellite locus used. The relative quantification of musts can be accomplished by the presented method depending on the presence of overlapping
alleles, and the composition of the varieties and their relative amounts. 相似文献