实时可视决策系统在渤海湾旅大油田开发中的应用

实时可视决策系统可以将接收到的现场数据实时传输到陆地，井眼轨迹和随钻测井资料可实时在三维地质油藏模型中显示，供钻完井和地质油藏专家综合分析、判断并及时下达指令指导现场施工。旅大油田运用实时可视决策系统成功钻成了6口水平井和4口水平分支井，实现了不钻领眼、井眼轨迹在最佳油层内成功着陆，并使水平井段尽可能沿着油层最佳位置钻进。例如在LD10—1-A25m水平分支井段的钻井过程中，利用实时可视决策系统，控制水平井眼轨迹在油层最佳位置钻进，获得了日产810M^3。的高产油气流。     

EO/EG装置生产过程中有机酸的生成原因分析

分析了环氧乙烷，乙二醇装置工艺系统中有机酸生成的原因．指出在银催化剂作用下，乙烯氧化过程有机酸生成主要成分是甲酸和乙酸，银催化剂不是影响氧化反应器出口醛含量的主要因素；杂质、温度等对反应器出口气中的醛含量有重要影响，也直接影响到装置前系统的有机酸含量；乙二醇后系统中有机酸的增加仅取决于两级吸收及解析工艺的稳定操作。     

同步式双螺杆泵螺杆瞬态动力学分析

螺杆是螺杆泵增压核心部件,其性能优劣对螺杆泵的整体性能具有决定性的影响。利用三维实体造型软件Pro/E建立准确实体模型,然后导入有限元分析软件ANSYS,建立了合理简化的螺杆瞬态分析模型,根据有限元思想将连续变化的压力承载面离散处理,利用ANSYSAPDL程序实现波动载荷的加载,从而实现转子瞬态动应力和响应特性的求解。     

两种在用发动机油氧化衰变情况监控方法对比分析

分析了两种常用监控在用发动机油氧化衰变情况的试验方法，并通过监控试验数据，探讨酸值和红外两种监测方法的各自特点，提出高档发动机油酸值变化极限值应区别于中低档发动机油的观点，为今后监控在用发动机油选用合适的试验方法和标准提供了参考。     

120万吨／年加氢裂化装置的开工及运行

介绍广州石化120×10^4t／a加氢裂化装置装置开工、运行过程以及满负荷标定结果。标定和运行情况表明，装置运行平稳，可以满足加工高硫直馏蜡油的要求，生产出优质轻质馏分油。     

Effect of steam on heavy oil catalytic pyrolysis

This paper studied the influence of steam on heavy oil catalytic pyrolysis. Experiments of Chinese Daqing atmospheric residue catalytic pyrolysis on CEP-1 were conducted in a confined fluidized bed reactor, with steam and nitrogen being used as the fluidizing gas, respectively. The effects of steam on the volume of contents of the components in pyrolysis gas, product yields, and carbon dioxide formation were discussed. The results of contrast tests showed that steam not only had the action of the fluidizing gas and the dilution gas, but also participated in steam gasification and water-gas shift reactions. In catalytic pyrolysis processes, steam could maintain the activity of catalysts, accelerate the reactions of carbonium ions, inhibit undesired secondary reactions, and enhance the yields and selectivity of the aimed products. Published in Russian in Neftekhimiya, 2007, Vol. 47, No. 2, pp. 99–102. The text was submitted by the authors in English.     

cDNA sequence and gene locus of the human retinal phosphoinositide-specific phospholipase-C beta 4 (PLCB4)

Defects in the Drosophila norpA (no receptor potential A) gene encoding a phosphoinositide-specific phospholipase C (PLC) block invertebrate phototransduction and lead to retinal degeneration. The mammalian homolog, PLCB4, is expressed in rat brain, bovine cerebellum, and the bovine retina in several splice variants. To determine a possible role of PLCB4 gene defects in human disease, we isolated several overlapping cDNA clones from a human retina library. The composite cDNA sequence predicts a human PLC beta 4 polypeptide of 1022 amino acid residues (MW 117,000). This PLC beta 4 variant lacks a 165-amino-acid N-terminal domain characteristic for the rat brain isoforms, but has a distinct putative exon 1 unique for human and bovine retina isoforms. A PLC beta 4 monospecific antibody detected a major (130 kDa) and a minor (160 kDa) isoform in retina homogenates. Somatic cell hybrids and deletion panels were used to localize the PCLB4 gene to the short arm of chromosome 20. The gene was further sublocalized to 20p12 by fluorescence in situ hybridization.     

Sanger DNA-sequencing reactions performed in a solid-phase nanoreactor directly coupled to capillary gel electrophoresis

A miniaturized, solid-phase nanoreactor was developed to prepare Sanger DNA-sequencing ladders which was directly interfaced to a capillary gel electrophoresis system. A biotinylated fragment of the rat brain actin gene (1 kbp) was amplified by PCR and attached to the interior wall of an (aminoalkyl)silane-derivatized fused-silica capillary tube via a biotin/streptavidin/biotin linkage. Coverage of the capillary wall with the biotinylated DNA averaged 77 +/- 10%. Stability of the anchored template under pressure (33 nL/s) and electroosmotic flows (11.3 nL/s) were favorable, requiring rinsing for > 150 h to reduce the surface coverage by only 50%. In addition, the immobilized template was stable toward temperatures required for preparing sequencing ladders, even under cycling conditions. Standard Sanger dideoxynucleotide termination performed in a large-volume (approximately 8 microL) solid-phase reactor using the thermally stable polymerase enzymes Taq and Vent and the polymerases T7 and Bst with off-line slab gel electrophoresis and autoradiographic detection indicated that acceptable fragment generation was achieved only in the case of the thermally stable polymerases. Banding was not apparent for T7 and Bst since all reagents were inserted into the column in a single plug at the beginning of the reaction. A small volume reactor (volume approximately 62 nL) was then used to perform DNA polymerase reactions and was coupled directly to a capillary gel column for separation. The capillary reactor was placed inside a thermocycler to control the temperature during chain extension and was directly connected to the gel column via zero dead volume fused-silica connectors. The complementary DNA fragments generated (C-track only) in the reactor were denatured using heat and directly injected onto the gel-filled capillary for size separation with detection accomplished using near-IR laser-induced fluorescence. Extension and single-base separation resolution of the C-track, which was directly injected onto the gel column, was estimated to be > 450 bases from the primer annealing site with plate numbers ranging from 1 x 10(6) to 2 x 10(6)/m.     

汽相平衡扩散时LiNbO_3晶体的改性

本文从ＬｉＮｂＯ３晶体的宽固溶特性出发，采用汽相平衡扩散（ＶＴＥ）方法，摸索出具体改善ＬｉＮｂＯ３组成的实验条件，研制出用于扩散的扩散源坩埚，对一系列同成分ＬｉＮｂＯ３晶体进行了改性研究，结果表明，经本方法处理的ＬｉＮｂＯ３晶体的均匀性、一致性得到大大的提高，并获得了均匀富锂的ＬｉＮｂＯ３晶体，能够很好地满足集成光学需要．