Silicon-based microelectrode arrays for stimulation and signal recording of in vitro cultured neurons

Microelectrode arrays (MEAs) for stimulation and signal recording of in vitro cultured neurons are presented.Each MEA is composed of 60 independent electrodes with 59 working ones and one reference one.These electrodes are divided into 30 pairs.Through each pair of electrodes,four independent states can be realized to define the accessing modes of neurons cultured on the surface of the electrodes.A total MEA covers an area of 10 mm×10 mm.MEAs are fabricated in a silicon-based semiconductor process.An implemented MEA is bonded on a specially designed printed-circuit-board (PCB) and surrounded by a culture chamber.An impedance measurement has been made to verify the electrical characteristics of MEAs.The surface was modified to enhance the biocompatibility.A series of PC12 cells culture experiments validates the effectiveness of the modification.An extracellular signal recording experiment with acetylcholine (Ach) as a stimulant has been carried out,and the results show the feasibility of MEAs for extracellular action potential recording.Extracellular electrical stimulation and recording experiments have been carried out too.They indicate that MEAs can be used for extracellular stimulation,recording,simultaneous stimulation and recording,and isolation of PC12 cells network cultured in vitro.     

Theory and techniques of data mining in CGF behavior modeling

For the large quantity of data,rules and models generated in the course of computer generated forces (CGFs) behavior modeling,the common analytical methods are statistical methods based on the tactical rules,tactical doctrine and empirical knowledge.However,from the viewpoint of data mining,we can find many of these analytical methods are also each-and-every different data mining methods.In this paper,we survey the data mining theory and techniques that have appeared in the course of CGF behavior modeling f...     

我国陆地观测卫星现状及发展战略思考

本文回顾总结了我国陆地观测卫星载荷体系、地面系统建设及数据应用等方面的发展现状和成就,分析了当前所面临的挑战和任务,以此为基础,对我国陆地观测卫星的未来发展进行了战略思考和展望,提出了加强国家顶层规划、加强地面系统统筹建设、实现系列化业务化运营、加强国际合作、推进产业化发展等建议.     

网络信息安全问题的法律探析

网络技术的发展以及应用深刻地影响着人类社会,在给人类带来巨大便利的同时也带来了诸如网络信息安全问题等隐患,这不仅在技术层面给我们提出新的问题,在法律层面也提出了更高的要求。本文介绍了对网络信息安全问题进行法理探讨的背景,并且在分析了国内外研究现状的基础上提出在法律层面解决网络信息安全问题的初步构想。     

Analysis of microwave brightness temperature of lunar surface and inversion of regolith layer thickness:Primary results of Chang-E 1 multi-channel radiometer observation

In China’s first lunar exploration project, Chang-E 1 (CE-1), a multi-channel microwave radiometer was aboard the satellite, with the purpose of measuring microwave brightness temperature from lunar surface and surveying the global distribution of lunar regolith layer thickness. In this paper, the primary 621 tracks of swath data measured by Chang-E 1 microwave radiometer from November 2007 to February 2008 are collected and analyzed. Using nearest neighbor interpolation based on the sun incidence angle in ...     

磁盘空间占用“大户”查看我最行

当计算机系统长时间运行之后,很多人或许会感觉到系统速度会越来越慢,硬盘剩余空间也越来越小了。特别是一些喜欢下载软件或欣赏视频的用户,常常会感觉到硬盘空间就像是被病毒侵蚀一样不知不觉就被消耗光了。     

识别并管理企业的应用个性

很多时候,IT预算只是用来管理并运行企业的基础设施,而不是推动业务创新。不能适应当今迅速变化步伐的老旧系统和应用更加剧了这一问题。这种方式往往会导致企业发展停滞不前,且难以满足不断变化的业务需求。将现代化应用组合进行整合并转换到全新架构将能促进企业发展,能显著提高其敏捷性及创新能力。     

单漂移聚束器的非标准聚束模式

单漂移聚束器标准聚束模式要求粒子注入能量和聚束器漂移管的几何尺寸之间必须满足严格的条件,严重地限制了可聚束粒子的种类。文章论述了采用非标准聚束模式的方法,计算不同粒子在HI-13串列加速器上实现脉冲聚束的可行性。     

Polycrystalline diamond MESFETs by Au-mask technology for RF applications

Metal-semiconductor field effect transistors (MESFETs) were fabricated on H-terminated polycrystalline diamond.The DC characteristics of the p-channel MESFET showed a maximum drain current density of 204 mA/mm at a gate-source voltage of 6 V,and a maximum transconductance of 20 mS/mm at a gate-source voltage of 1.5 V.The small signal S-parameters of MESFET with 2 100 m gate width and 2 m gate length were measured.An extrinsic cut-off frequency (fT) of 1.7 GHz and the maximum oscillation frequency (fmax) of 2.5 GHz were obtained,which was the first report on diamond MESFETs with RF characteristics in China.     

Numerical investigation on flow and convective heat transfer of aviation kerosene at supercritical conditions

In this paper,characteristics of flow and convective heat transfer of China RP-3 kerosene in straight circular pipe were numerically studied.Navier-Stokes equations were solved using RNG k-turbulence model with low Reynolds number correction.The thermophysical and transport properties of the China RP-3 kerosene were calculated with a 10-species surrogate and the extended corresponding state method(ECS) combined with Benedict-Webb-Rubin equation.The independence of grids was first studied and the numerical results were then compared with experimental data for validation.Under flow conditions given in the paper,the results show that deterioration of convective heat transfer occurs when the wall temperature is slightly higher than the pseudo-critical temperature of kerosene for cases with wall heat flux of 1.2 and 0.8 MW/m 2.The degree of the heat transfer deterioration is weakened as the heat flux decreases.The deterioration,however,does not happen when the heat flux on the pipe wall is reduced to 0.5 MW/m 2.Based on the analysis of the near-wall turbulent properties,it is found that the heat transfer deterioration and then the enhancement are attributed partly to the change in the turbulent kinetic energy in the vicinity of pipe wall.The conventional heat transfer relations such as Sieder-Tate and Gnielinski formulas can be used for the estimation of kerosene heat convection under subcritical conditions,but they are not capable of predicting the phenomenon of heat transfer deterioration.The modified Bae-Kim formula can describe the heat transfer deterioration.In addition,the frictional drag would increase dramatically when the fuel transforms to the supercritical state.