Amphibians as a model to study endocrine disruptors: I. Environmental pollution and estrogen receptor binding

Many chemicals released into the environment without toxicological risks have the capacities to disrupt the function of endocrine systems. These endocrine disruptors disturb normal endocrine mechanisms and have been observed in nearly all classes of vertebrates. The aim of this research is to develop a comprehensive model to study endocrine disruption using the amphibian Xenopus laevis. The assessment of estrogenic potencies of endocrine disruptors includes several levels of investigation: (I) binding to liver estrogen receptor, (II) estrogenic activity in vitro by inducing vitellogenin synthesis in primary cultured hepatocytes, and (III) in vivo effects on sexual development caused by exposure of larvae. The present paper is focused on the first part by establishing a radioreceptorassay for [3H]17 beta-estradiol ([3H]E2) binding using liver cytosol fraction. In order to get optimum binding conditions we performed kinetic, saturation, and competitive displacement experiments. Association of [3H]E2 to estrogen receptor revealed that maximum specific binding is achieved between 18 and 48 h of incubation. Scatchard analyses of saturation experiments resulted in a homogenous saturable population of estrogen receptors having no significant differences of binding parameters between both sexes. The values of Kd (dissociation constant) in males and females were 22.4 +/- 6.0 and 15.0 +/- 2.8 nM (mean +/- S.E.M.; n = 5), respectively, while corresponding Bmax (maximum binding capacity) revealed 89 +/- 46 and 136 +/- 46 fmol [3H]E2/mg protein. The specificity of estrogen receptors as shown by competitive displacement experiments demonstrated receptors being highly specific just for estrogens, but not for other endogenous steroids having the following ranking of binding affinities: E2 > estrone > dehydroepiandrosterone > aldosterone > or = testosterone > or = corticosterone > or = progesterone. The affinity ranking of environmental chemicals compared to E2 was: E2 > tetrachlorbiphenyl > diethylphthalate > 2,2-bis-(4-hydroxyphenyl)-propan (bisphenol A) > or = 4-nonylphenol > or = 3-t-butyl-4-hydroxyanisole > or = 4-octylphenol > dichlor-diphenyl-trichlor-ethan (4,4'-DDT). Analyses of five sewage effluents for displacement of [3H]E2 binding resulted in three samples displacing more than 50% of specific binding at their original concentration. Taken together the established radioreceptorassay for [3H]E2 binding in Xenopus laevis liver cytosol is useful to screen estrogen receptor binding of pure compounds or complex mixtures of them, which is the prerequisite for causing either estrogenic or antiestrogenic effects.     

What can be taught in architectural design? — <Emphasis Type="Italic">parti,poché</Emphasis>, and felt qualities

This essay begins with a reflection on what has been taught in architectural design since the turn of the twentieth century. I shall trace back to the two disciplinary foundations of the French École des Beaux-Arts — parti and poché — in the education of an architect in the eighteenth and nineteenth centuries. I shall then attempt to superimpose parti and poché on a modern disciplinary framework, say that of mathematics, which leads to musings on a series of architectural problems that include pattern versus type, stability versus mobility, orthogonal versus oblique, confinement versus transparency, and aging versus metallic sheen. These paradoxes, I suggest, demand the education of an architect to address both the instrumental pattern of a building configuration and the ambient felt qualities of a room, rather than vision alone.     

An experimental determination of combustibility

The author describes a laboratory method for determining ignitability, rate of heat release, and total heat release as a measure of the combustibility of materials. The results may be applied to building design and designation of contents with an eye towards reducing the possibility of a major fire. Note: Professor Smith is Director of Chemical Engineering Research, Engineering Experiment Station, The Ohio State University, Columbus, Ohio.     

Spatial competition and regulatory change in the grain handling and transportation system in western Canada

The grain handling and transportation system (GHTS) has been a defining component of the western Canadian economy since the early part of the last century. Only two major railways serve the grain handling industry, so the potential for the exercise of market power in transportation appears to be significant. In fact, spatially oriented regulations applicable to railways exist in Canada to mitigate this, but to date they have not often been used by shippers. While the reasons for this are not completely clear, we suggest that the regulations are now inadequate for the current state of the GHTS. Using a unique data set from the year 2000, we assess the effect on the grain handling industry of modifications to these regulations, and find that significant gains for shippers from these modifications are possible. These gains are computed to be on the order of C$10–15 million per year for grain shippers alone. We would like to thank conference and presentation participants (Transportation Research Board, Canadian Agricultural Economics Society, University of British Columbia, University of Leeds and California State University, Long Beach) as well as the anonymous reviewers who commented on the earlier versions of this paper. We would also like to acknowledge support from the Saskatchewan Department of Highways.     

Geomechanical model simulation for the varied rock formations of the Kadana dam foundation,Gujarat, India

Bulletin of Engineering Geology and the Environment - Precambrian layered rocks consisting of quartzites, phyllites and metagreywackes of the Aravallis having a large variation in thickness,...     

Decomposing regional productivity growth using an aggregate production frontier

The main contribution of this paper is the implementation of a new model which combines the two parametric approaches most commonly used in the productivity literature: fixed effects and stochastic frontiers. This allows us to discuss whether it is better to use average or frontier functions to estimate regional productivity. The empirical section uses panel data of Spanish regions over the period 1980–1995. Additionally, we calculate and decompose total factor productivity growth for the Spanish regions.     

The development of macrolayer thickness of water in the pool boiling regime

ABSTRACT

The main purpose of this study is to analyse the phenomenon of pool boiling in water over a horizontally placed copper tube heater of 28?mm diameter. The experiment has been carried out to observe the bubble growth and departure characteristics for the heat flux range up to 40,000?W/m². The entire process is being recorded by a digital camera at different time intervals. The measured parameters have been used to determine the initial layer thickness, macro layer thickness and critical heat flux and validated with models proposed in the literature.