粗壮脉纹孢菌孢子中类胡萝卜素的体外发酵特性

模拟人体消化过程对粗壮脉纹孢菌孢子进行消化,通过酸热法提取消化后孢子粉中的类胡萝卜素,进行体外发酵。采用国标法测定发酵液的产气量、pH、NH3-N,采用气相色谱法测量发酵液中短链脂肪酸的含量。通过16SrRNA高通量测序技术分析志愿者肠道菌群,结果发现消化后的粗壮脉纹孢菌孢子粉中类胡萝卜素经体外发酵,pH值趋于6.0~7.2,与人体肠道pH值接近,产气量较少,不易引起胀气,可产生的乙酸、丙酸、丁酸等有益于人体健康的短链脂肪酸,使拟杆菌减少,变性杆菌增加。粗壮脉纹孢菌孢子粉中类胡萝卜素有利于改善人体肠道菌群,优化肠道菌群中各菌属的比例,有益于人体的肠道健康。     

5种因子对裂褶菌菌丝生长及胞外多糖产生的影响

研究了VB1、α 萘乙酸、油酸、羧甲基纤维素钠、L 谷氨酸 5种因子对裂褶菌菌丝生长及胞外多糖产生的影响。当VB1的添加量为 0 5mg/L ,α 萘乙酸的添加量为 0 2mg/L ,油酸浓度为0 1 % ,羧甲基纤维素钠的添加量为 0 6% ,L 谷氨酸的添加量为 1 0 0 0 μg/L时均能明显促进菌丝生长和胞外多糖的产生。裂褶菌菌丝体生长和胞外多糖的产生具有正相关性。     

大学生就业法律制度建立与完善的思考

目前,我国的社会主义法律体系已经基本建成,其中对于就业保障的相关法律也得到相应补充和完善。然而,在社会经济体制的合理化发展的大背景下,为合理有效解决我国就业问题,相关的部门不断地建立和完善就业法律制度仍是社会发展的必然趋势。在现有的社会经济体制下,我国的劳动市场在就业方面存在一定的问题,国有企业、私人企业以及集体企业等的发展都需要法律的支持和保护,因此,需要借鉴世界各国的成功经验,不断地加强立法,建立健全相关的就业法律制度,保证就业市场的稳定发展。     

花生油加工和相关技术研究进展及展望

综述了近年来我国在花生油传统加工工艺提升、花生油加工新技术、花生油黄曲霉毒素控制技术、花生油掺假鉴定技术和油脂抗氧化技术研究等方面取得的研究进展,提出了花生油加工技术的发展方向并展望了加工新技术的应用前景。     

Effects of Enzymatic Hydrolysis of Protein on the Pasting Properties of Different Types of Wheat Flour

Abstract: As one of the most effective methods to modify proteins, enzymatic hydrolysis is used widely in the preparation of wheat products in the food industry. During the same process, starch pasting occurs frequently. The effects of wheat protein hydrolysis with papain, pepsin, and trypsin on the pasting properties of 3 different kinds of flour were investigated in 5 concentrations. Results showed that the peak viscosity, trough, final, and integral area of pasting curve of these flours decreased with increasing enzymatic hydrolysis of protein, and decreased significantly with the increasing enzyme concentrations. Medium‐gluten flour was the least sensitive to enzymatic activity and weak‐gluten the most sensitive. Downtrends appeared with increasing papain and trypsin concentrations in the form of breakdown. Enzymes had no significant different effect on the peak times of strong‐ and medium‐gluten flour, but prolonged peak time slightly in weak‐gluten flour. The pasting time and temperature of strong‐ and medium‐gluten flour were significantly increased in a concentration‐dependent manner. However, there were no significant effects on the pasting times of weak‐gluten flour. These results could supply a basis for utilization of enzymatic hydrolysis of wheat protein in food industry and for further studies into the interactions between hydrolyzed protein and starch in food or processing industries. Practical Application: Illuminating the effects of enzymatic hydrolysis of protein on the pasting properties of different types of wheat flour is very important in food industry. Flour viscosity decreases after enzymatic hydrolysis of protein/gluten. Enzymes have different effects on peak time, pasting time, and pasting temperatures for different types of flour. These results could supply a basis for utilization of enzymatic hydrolysis of wheat protein in food industry and for further studies into the interactions between hydrolyzed protein and starch in food or processing industries.     

石花菜醇提物提取工艺及抗氧化、抗菌活性研究

在单因素实验基础上,通过响应面法优化石花菜醇提物的提取工艺。将石花菜醇提物经石油醚、乙酸乙酯和正丁醇依次萃取,研究不同萃取相的抗氧化和抑菌活性。结果表明,石花菜醇提物的最佳提取工艺为:60%乙醇溶液、料液比1∶35、提取时间64 min、超声功率350 W、提取温度55 ℃。在此条件下,石花菜醇提物得率为0.0165 g/g,DPPH自由基清除率为58.29%(质量浓度2 mg/mL)。不同萃取相抗氧化和抗菌活性均具有浓度依赖性,其中乙酸乙酯相活性最强,清除DPPH自由基、ABTS+自由基和羟基自由基的IC50值分别为672.4,14.1,599.7 μg/mL。对金黄色葡萄球菌和副溶血性弧菌的最小抑菌浓度(MIC)分别为156.3,156.3 μg/mL,最小杀菌浓度(MBC)分别为625,312.5 μg/mL。     

采用响应面法优化木糖醇发酵培养基

将Plackett-Burman和响应面设计相结合,对木糖醇发酵培养基进行了优化。结果表明,初始木糖浓度、酵母膏添加量以及MgSO4.7H2O浓度是影响木糖醇转化率的主要因素。优化得到的培养基组成为(g/L)木糖100.7,酵母膏5.302,NaCl6.0,MgSO4.7H2O0.379,KH2PO43,(NH4)2HPO44;通气条件为装液量100mL/250mL。此条件下木糖醇的转化率为0.784g/g。     

高速试验滑轨工程设计研究

高速试验滑轨是一种大型地面动态模拟试验设备,主要用于研究试验件在空中高速度、高加速度运行时所遇到的诸多技术问题,其核心技术是十万分之一的直线性相对精度。本文较为详细地阐述了滑轨子系统、承载梁子系统以及高精度测量子系统的设计原理和要点。其中双向受力锚固机制、承载梁子系统中的整体式道床方案以及高精度激光跟踪仪技术均为该领域的创新技术。     

南京工程学院图书信息中心能耗现状分析

由于能耗统计和分析的缺失,设计者凭主观决策的设计并不一定能达到预期的效果。以南京工程学院逸夫图书信息中心作为研究对象,分析其2014和2015年两年的能耗数据,结合实地能耗数据的调研以及使用方的反馈,通过实践数据来检验初始设计,以期得到更为客观的图书馆低能耗设计策略。分析过程中采用时间轴上年度间的纵向比较,分析图书馆能耗与气温和运营管理的关系;通过与其他大学图书馆横向的比较,准确定位分项能耗水平,引导分析设计策略。     

微波消解石墨炉原子吸收法测定食用油中铬和镉

本实验专门针对食用油开发出以微波消解-原子吸收石墨炉法准确测定油品中重金属镉、铬的实验方法,研究了油品消解的最佳处理方法及消解条件。通过基体改进剂和灰化温度的改善确定了原子吸收最佳工作条件,方法的相对偏差低于4.04%,仪器检出限为0.01μg/kg,加标回收率在95.0%~102.8%之间。实验结果表明此方法准确、检出限低、灵敏度高、分析结果令人满意。