Resource management with virtual paths in ATM networks

A virtual path connection (VPC) is a labelled path which can be used to transport a bundle of virtual channel connections (VCCs) and to manage the resources used by these connections. The virtual network is organized as a collection of VPCs which form a VPC, or logical, overlay network. If the VPCs are permanent or semi-permanent and have reserved capacity, establishing new VCCs requires simple connection admission decisions at the VPC terminators of existing VPCs. This would enable faster connection establishment since transit nodes are not involved in the connection setup. The virtual path concept also allows the possibility of segregating traffic types according to quality of service requirements. However, the extent to which VPC provisioning is able to improve network efficiency is dependent on the resource management decisions that determine the VPC topology and capacity allocations. The article surveys resource management using virtual paths in an ATM network. Of interest are techniques which modify the VPC topology and capacity assignments in order to adapt to changing traffic conditions and possible network failures. The resource management activities employed to facilitate such adaptation can be categorized by the timescale on which they operate. On the shortest timescale are strategies for dynamically making minor changes to the VPC topology or capacity assignments. On a somewhat longer timescale are strategies for making more widespread modifications to the VPC overlay network. This would be appropriate for traffic changes based on time of day and for recovering from network failures. Finally, on an even longer timescale, strategies may be employed to design a general VPC overlay network, to be used at startup or after major network upgrades. Solutions to VPC resource management for each of these timescales are discussed     

Allostery in rabbit pyruvate kinase: development of a strategy to elucidate the mechanism

Isozymes of pyruvate kinase (PK) expressed in rabbit muscle and kidney show different allosteric kinetics. The only amino acid changes in the two isozymes, originating from alternative RNA splicing, occur at a stretch of 55 amino acids in the C domain near the subunit interface. The self-correcting distance geometry (SECODG) program DIAMOD was used to calculate a homology model of these interfacial contacts in the four helix bundle of the kidney PK dimer, based on the X-ray structure of the tetrameric rabbit muscle PK [Larsen et al. (1994) Biochemistry 33, 6301-6309]. Energy refinement with the program FANTOM, using the ECEPP/2 force field to assess packing and electrostatic interactions between the two subunits, yielded two groups of energetically favorable conformations. The primary difference in the two groups is the loop conformation of residue Pro 402, which is serine in muscle PK. In one loop conformation, the conserved Lys 421 can form an intersubunit salt bridge as observed in the muscle PK crystal structure. The other loop conformation favors an alternative intrasubunit salt bridge, similar to that found in the Escherichia coli PK structure, which was not used for generating the model. The intersubunit salt bridge leads to an intersubunit hydrogen bonding between Lys 421 of one subunit and Tyr 443 of the other. To provide direct evidence on the roles of these residues, site-directed mutagenesis of the muscle PK gene was conducted. Converting Ser 402 to a proline and Tyr 443 to a phenylalanine changed neither the secondary nor the tetrameric structure, as measured by far UV-CD and sedimentation velocity, respectively. However, the S402P mutant exhibits steady-state kinetics, indicating that the mutant is more reponsive to regulation by effectors, while the mutant Y443F was essentially equivalent to wild-type muscle PK protein except for a lower affinity to phosphoenolpyruvate. These findings suggest a pivotal role for a few key residues in the allosteric regulation in PK.     

Influence of infused prolactin on hormone binding to tissue slices

The influence of circulating prolactin on the binding of labeled hormone to tissue slices in vitro was investigated in rats bearing dimethylbenzanthracene-induced mammary tumors. Prolactin was infused into four animals over four 30-min periods to give serum levels ranging from 20 to 2000 ng/ml. Serial biopsies of liver and tumor were taken during infusion, and binding of labeled prolactin and insulin measured in tissue slices. Whereas insulin binding remained constant, prolactin binding was significantly depressed in both tissues when samples were obtained at serum prolactin levels greater than 300 ng/ml. This decrease was due to diminished numbers of available binding sites rather than a change in binding-site affinity for prolactin. The data indicate that under the conditions of this experiment, endogenous circulating prolactin may interfere with in vitro measurements of hormone uptake when serum levels excee 300 ng/ml.     

A tool for the analysis of motion picture film or video tape.

Describes the Visual Information Display and Retrieval (VID-R) system, designed for the analysis of visual records, archiving, and visual displays in programed instruction. The necessities for permanent visual records are reviewed. Employment of VID-R functions of (a) viewing speed, (b) search and retrieval, (c) temporal reorganization (editing), and (d) presentation of a visual dictionary is explained. Components "incorporate as much off-the-shelf hardware as possible" and contain maximum flexibility in logic. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Untersuchung der Bainitumwandlung in Siliciumstahl

Untersuchung der Morphologie des unteren Bainitbereichs in gegossenem Siliciumstahl mit 0,83% C und 2,55% Si durch Durchstrahlungselektronenmikroskopie nach Austenitisieren und isothermischem Umwandeln (Haltedauer 30 s bis 100 h) bei einer Temperatur von 300°C. Feststellung der kristallographischen Beziehungen zwischen bainitischem Ferrit, Restaustenit, ?-Carbid und Zementit.