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81.
Shin-ichi Osawa Akira Furuzawa Nobuyoshi Fujikawa 《Journal of the American Ceramic Society》1993,76(5):1191-1194
The effect of defect structure on electrical conductivity for Mn-doped BaTiO3 at high temperatures was studied experimentally and theoretically. The measured conductivity was proportional to Po2 −1/4 in the n -type region, and to P o2 +1/6 in the p -type region in the temperature range between 900° and 1000°C. The results agree with calculations on the basis of the equilibrium equation for the valence change of Mn ions between tetravalent and trivalent. In the p -type region, the concentration of trivalent Mn ions decreases with P o2 −1/6 . 相似文献
82.
Knowledge of the changes in the behavior of human soft tissue stiffness during physical impact in human–machine interaction (HMI) plays a vital role in the development of biofidelity testing devices such as a human dummy. These testing devices are widely applied as an effective means to validate the safety of machinery during dynamic or static contact with humans in HMI. In this study, we assess changes in soft tissue stiffness in the upper arm of individuals under both dynamic (0.7 and 0.25 m/s) and quasi-static (QS) impacts under a constrained contact condition. Three impactor shapes (cylindrical, cubic, and spherical) are used in this study. Impact experiments are conducted using impactors attached to a pendulum. The soft-tissue displacement is determined using an ultrasound device. The impact force-displacement curves illustrate the nonlinear behavior of the soft tissue stiffness under both dynamic and QS impacts. By utilizing the “Linear Mixed Model” statistical analysis, we found that changes in the impact velocity significantly influenced the changes in the nonlinear behavior of soft tissue stiffness while there was no significant effect of the changes in the impactor shape on the nonlinear behavior of the soft tissue stiffness. Additionally, we revealed that the changes in the soft tissue stiffness are influenced by the size of the contact area. Moreover, we demonstrated a range of changes in soft tissue stiffness for different impact velocities, which provide valuable information for developing future validation test devices in HMI, such as the design and evaluation of dummy skin. 相似文献
83.
84.
The effects of additions of as little as 0.02%Nito mild steel on the morphology of the scale-steelinterface and surface cracking were studied duringhigh-temperature oxidation and subsequent hot-ductility tests of 0.5%Cu-bearing steel. Liquid-Cuembrittlement occurs in a steel containing0.5%Cu-0.001%Ni because of concentrated liquid-Cu layerat the scale-steel interface over the temperature rangeof 1100-1300°C. The addition of as little as0.023%Ni to 0.50%Cu-containing steel recovers theductility in the oxidation temperature range of1250-1300°C. This is because liquid-Cu particles areoccluded into the scale. Thus, the amount of enriched Cuper unit surface area decreases at the scale-steelinterface. It is suggested that liquid-Cu particleocclusion, which has not yet been elucidated, results from heterogeneous enrichment of Ni andheterogeneous oxidation at the scale-steelinterface. 相似文献
85.
86.
Mahadi Hasan Noriko Tarashima Koki Fujikawa Takashi Ohgita Susumu Hama Tamotsu Tanaka 《Science and Technology of Advanced Materials》2016,17(1):554-562
An intelligent shRNA expression device (iRed) contains the minimum essential components needed for shRNA production in cells, and could be a novel tool to regulate target genes. However, general delivery carriers consisting of cationic polymers/lipids could impede function of a newly generated shRNA via electrostatic interaction in the cytoplasm. Recently, we found that faint electric treatment (fET) of cells enhanced delivery of siRNA and functional nucleic acids into the cytoplasm in the absence of delivery carriers. Here, we examined fET of cells stably expressing luciferase in the presence of iRed encoding anti-luciferase shRNA. Transfection of lipofectamine 2000 (LFN)/iRed lipoplexes showed an RNAi effect, but fET-mediated iRed transfection did not, likely because of the endosomal localization of iRed after delivery. However, fET in the presence of lysosomotropic agent chloroquine significantly improved the RNAi effect of iRed/fET to levels that were higher than those for the LFN/iRed lipoplexes. Furthermore, the amount of lipid droplets in adipocytes significantly decreased following fET with iRed against resistin in the presence of chloroquine. Thus, iRed could be a useful tool to regulate target genes following fET-mediated cytoplasmic delivery with endosomal escape devices. 相似文献
87.
YH Tan JA Davis K Fujikawa NV Ganesh AV Demchenko KJ Stine 《Journal of materials chemistry》2012,22(14):6733-6745
Nitrogen adsorption/desorption isotherms are used to investigate the Brunauer, Emmett, and Teller (BET) surface area and Barrett-Joyner-Halenda (BJH) pore size distribution of physically modified, thermally annealed, and octadecanethiol functionalized np-Au monoliths. We present the full adsorption-desorption isotherms for N(2) gas on np-Au, and observe type IV isotherms and type H1 hysteresis loops. The evolution of the np-Au under various thermal annealing treatments was examined using scanning electron microscopy (SEM). The images of both the exterior and interior of the thermally annealed np-Au show that the porosity of all free standing np-Au structures decreases as the heat treatment temperature increases. The modification of the np-Au surface with a self-assembled monolayer (SAM) of C(18)-SH (coverage of 2.94 × 10(14) molecules cm(-2) based from the decomposition of the C(18)-SH using thermogravimetric analysis (TGA)), was found to reduce the strength of the interaction of nitrogen gas with the np-Au surface, as reflected by a decrease in the 'C' parameter of the BET equation. From cyclic voltammetry studies, we found that the surface area of the np-Au monoliths annealed at elevated temperatures followed the same trend with annealing temperature as found in the BET surface area study and SEM morphology characterization. The study highlights the ability to control free-standing nanoporous gold monoliths with high surface area, and well-defined, tunable pore morphology. 相似文献
88.
Fujikawa H Yano K Morozumi S Kimura B Fujii T 《Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan》2006,47(6):288-292
A predictive program for microbial growth under various temperature conditions was developed with a mathematical model. The model was a new logistic model recently developed by us. The program predicts Escherichia coli growth in broth, Staphylococcus aureus growth and its enterotoxin production in milk, and Vibrio parahaemolyticus growth in broth at various temperature patterns. The program, which was built with Microsoft Excel (Visual Basic Application), is user-friendly; users can easily input the temperature history of a test food and obtain the prediction instantly on the computer screen. The predicted growth and toxin production can be important indices to determine whether a food is microbiologically safe or not. This program should be a useful tool to confirm the microbial safety of commercial foods. 相似文献
89.
Yuji Shimada Akio Sugihara Yumi Minamigawa Kenichi Higashiyama Kengo Akimoto Shigeaki Fujikawa Sadao Komemushi Yoshio Tominaga 《Journal of the American Oil Chemists' Society》1998,75(12):1213-1217
An attempt was made to enrich arachidonic acid (AA) from Mortierella single-cell oil, which had an AA content of 25%. The first step involved the hydrolysis of the oil with Pseudomonas sp. lipase. A mixture of 2.5 g oil, 2.5 g water, and 4000 units (U) Pseudomonas lipase was incubated at 40°C for 40 h with stirring at 500 rpm. The hydrolysis was 90% complete after 40 h, and the resulting free fatty acids (FFA) were extracted with n-hexane (AA content, 25%; recovery of AA, 91%). The second step involved the selective esterification of the fatty acids with lauryl alcohol and Candida rugosa lipase. A mixture of 3.5 g fatty acids/lauryl alcohol (1:1, mol/mol), 1.5 g water, and 1000 U Candida lipase was incubated at 30°C for 16 h with stirring at 500 rpm. Under these conditions, 55% of the fatty acids were esterified, and the AA content in the FFA fraction was raised to 51% with a 92% yield. The long-chain saturated fatty acids in the FFA fraction were eliminated as urea adducts. This procedure raised the AA content to 63%. To further elevate the AA content, the fatty acids were esterified again in the same manner with Candida lipase. The repeated esterification raised the AA content to 75% with a recovery of 71% of its initial content. 相似文献
90.
H Hamaguchi K Nagata K Yamamoto I Fujikawa M Kobayashi M Eguchi 《Canadian Metallurgical Quarterly》1998,102(5):1249-1256
Calcein-labeled B16 melanoma (B16M) cells were injected intraportally, and in vivo video microscopy was used to study the distribution and damage of cancer cells arrested in the liver microvasculature over a period of 4 hours. The contribution of glutathione (GSH)-dependent antioxidant machinery to the possible oxidative stress-resistance mechanism of B16M cell was determined by in vitro incubation with the selective inhibitor of GSH synthesis L-buthionine (S,R)-sulphoximine (BSO) before B16M cell injection in untreated and 0.5-mg/kg lipopolysaccharide (LPS)-treated mice. In addition, untreated and LPS-treated isolated syngeneic hepatic sinusoidal endothelial cells (HSE) were used to determine in vitro their specific contribution to B16M cell damage. Trauma inherent to intrasinusoidal lodgement damaged 35% of B16M cells in both normal and LPS-treated mouse liver. The rest of the arrested B16M cells remained intact in normal liver for at least 4 hours, although their damaged cell percentage significantly (P < .05) increased since the second hour in normal mice injected with BSO-treated cells and since the first hour in LPS-treated mice given untreated cells. Recombinant human interleukin-1 receptor antagonist (rHuIL-1-Ra) given to mice 15 minutes before LPS significantly (P < .05) abrogated B16M cell damage. On the other hand, 40% of the B16M cells co-cultured with unstimulated HSE and 70% of the co-cultured with LPS-treated HSE became sensitive to endothelial cell-mediated damage after BSO treatment. These results demonstrate that a high intracellular level of GSH protects B16M cells from possible in vivo and in vitro sinusoidal cell-mediated oxidative stress, contributing to the mechanism of metastatic cell survival within the hepatic microvasculature. 相似文献