Immunohistochemical identification of varicella-zoster virus gene 63-encoded protein (IE63) and late (gE) protein on smears and cutaneous biopsies: implications for diagnostic use

Early and specific recognition of varicella zoster virus (VZV) infection is of vital concern in immunocompromised patients. The aim of this study was to compare the diagnostic accuracy of histochemical and immunohistochemical identification of the VZV ORF63 encoded protein (IE63) and of the VZV late protein gE on smears and formalin-fixed paraffin-embedded skin sections taken from lesions clinically diagnosed as varicella (n = 15) and herpes zoster (n = 51). Microscopic examinations of Tzanck smears and skin sections yielded a diagnostic accuracy of Herpesviridae infections in 66.7% (10/15) and 92.3% (12/13) of varicella, and 74.4% (29/39) and 87.8% (43/49) of herpes zoster, respectively. Immunohistochemistry applied to varicella provided a type-specific virus diagnostic accuracy of 86.7% (13/15; IE63) and 100% (15/15; gE) on smears, and of 92.3% for both VZV proteins on skin sections. In herpes zoster, the diagnostic accuracy of immunohistochemistry reached 92.3% (36/39; IE63) and 94.9% (37/39; gE) on smears, and 91.7% (44/48; IE63) and 91.8% (45/49; gE) on skin sections. These findings indicate that the immunohistochemical detection of IE63 and gE on both smears and skin sections yields a higher specificity and sensitivity than standard microscopic assessments.     

Combining beta-core fragment and total oestriol measurements to test for Down syndrome pregnancies

Recent articles by Cuckle et al., Canick et al., and Isozaki et al. have evaluated urine beta-core fragment as a screening test for Down syndrome in second-trimester pregnancies. They found over four-fold elevation of beta-core fragment levels in Down syndrome pregnancies, and between 62 and 88 per cent detection of this trisomy at a 5 per cent false-positive rate. Urine beta-core fragment may be a superior screening test for Down syndrome pregnancies. In the present study, urinary total oestriol has been evaluated as a marker to use in combination with beta-core fragment in screening for Down syndrome pregnancies. The two markers were evaluated separately in relation to the urine creatinine concentration. To amplify screening performance, we evaluated the ratio of beta-core fragment to total oestriol levels (creatinine-independent). beta-core fragment and total oestriol levels were determined (normalized to creatinine, ng/mg creatinine) in urine samples from 480 unaffected and 12 Down syndrome pregnancies, collected consecutively at a single prenatal diagnosis centre. The median beta-core fragment level in Down syndrome cases was 4.5 MOM. Fifty-eight per cent of Down syndrome cases had beta-core fragment levels exceeding the 95th centile of unaffected pregnancies. The median total oestriol level in Down syndrome cases was 0.33 MOM. Forty-two per cent of Down syndrome cases had total oestriol levels exceeding the 95th centile of unaffected pregnancies. We investigated the ratio of the two determinants (beta-core fragment, ng/ml divided by total oestriol, ng/ml) in our sample set. The median beta-core fragment:total oestriol ratio in Down syndrome cases was 13 MOM. Seventy-five per cent of Down syndrome cases had a ratio exceeding the 95th and the 99.5th centile of unaffected pregnancies. Total oestriol complements beta-core fragment in urine screening for Down syndrome pregnancies. A test measuring the ratio of the two urine determinants may be a significant improvement over current serum methods for detecting Down syndrome.     

过氧化氢爆炸事故浅析

通过两起过氧化氢(双氧水)爆炸事故，分析了过氧化氢由于本身具有较大的潜能，很不稳定，当受热、泄漏、撞击、摩擦时，易分解放热产生高温，造成火灾爆炸事故，对火灾爆炸反应过程与爆炸机理进行了探讨。     

活塞式压缩机吸排气管道的振动与控制

阐述了活塞式压缩机吸、排气管道强烈振动的危害，分析了产生共振的原因，介绍了现场消减管道振动的有效措施。     

高校组织建模方法研究

从高校组织元素的构成与描述方法研究入手,分别建立了高校组织机构视图和高校办学活动过程模型.通过人流、物流、资金流、信息流在组织机构视图与办学活动过程模型之间的交换,建立了各组织元素之间的链接关系,从而构建出了整体性、系统性的高校组织模型.引入学科专业作为建模构件,通过基于进程的办学活动过程分析,反映了高校组织的特点,基本满足了高校组织建模的复杂性、特异性要求.     

编辑出版自动化技术在医药专业期刊编辑部的初步应用探讨

本文首先概述了编辑出版自动化技术的概念及最新进展,然后就其在医药专业期刊编辑部的初步应用进行了探讨,论述了计算机、网络、数据库技术在稿件的选题、组稿、编辑、审校、排版、印刷、出版中的具体应用,强调了构建基于网络的编辑出版自动化系统对期刊现代化建设的重要作用。     

B4C涂层作为等离子体面对材料的一些性质

介绍了利用等离子喷涂设备,B4C/Cu梯度功能涂层及非梯度涂层的制备技术,在模拟实验装置中测量了在高能粒子(3 keV,D+)的作用下,B4C涂层的化学溅射产额、热解吸性能、热冲击性能及热导率.结果表明B4C是一种有希望为未来聚变装置中等离子体面对的材料.     

高维滞环多值电路唯一稳态的研究

本文利用矩阵分解方法，在用常数界定元件成份关系斜率条件下，得到了确定高维具有滞环的非线性非自治电路唯一稳态的新条件。本文的结果表明，该类电路的唯一稳态，可以用分解矩阵的稳定性来决定，本文的结果中，电路元件可以是多值元件，这大大放松了目前已的经典结果中要求电路元件为单值的限制，扩展了已有的结果，同时，本文的结果，对于确定高维非线性非自治电路的唯一稳态，比已有结果，更加有力和简洁。     

分散网络制造浅谈

网络社会的制造业将是一个全球化的分散型的制造体系.分散网络制造是基于互联网的制造活动,因此,它具开放的、虚拟的,以及资源和技术更合理配置的特点.其组织模式是多种多样的,按现今国防工业现状,宜采用项目领导小组统一协调和控制下的分阶段动态联盟更为合适.为此,分析了网络无序化、企业属性知识不足、利益分配不易等问题带来的困难,并提出了一些建议.     

自动成图在工程设计中的应用

利用AutoCAD与高级语言的接口方式，编制程序在工程设计中自动成图。