康宁木霉cbh2基因在毕赤酵母中的表达

为拓宽酵母菌的应用领域,构建具有分泌纤维素酶能力的酵母工程菌,利用质粒pPICZαA构建了康宁木霉纤维素酶cbh2基因的酵母表达载体,经线性化后采用电穿孔法转入毕赤氏酵母菌中.以甲醇为诱导物进行诱导,聚丙烯酰胺凝胶电泳检测结果证明该基因可成功表达.对重组蛋白进行了产酶活性测定,结果证明该重组蛋白具有纤维二糖水解活性,从而获得了一株可产生纤维素酶的酵母工程菌.     

预应力钢结构的索力模拟分析方法

采用比值索力迭代法模拟预应力钢结构的索力,对半刚性和柔性结构不能得到正确的计算结果。构建了差值补偿索力模拟方法,对半刚性和柔性结构适应性好,计算稳定,较比值索力迭代法适用范围更广泛。使用有限元软件ANSYS中的参数化设计语言APDL编制了索力模拟分析程序,通过算例验证了差值索力补偿法迭代过程总是能稳定收敛到设计索力。引入的加速因子有效加快了迭代计算的收敛速度,对于本算例β取1.6～1.8时效果最佳。     

自升式海洋钻井平台锁紧装置液压系统

针对自升式海洋钻井平台锁紧装置,讨论其构成,对作为驱动装置的液压系统进行设计并作出原理图,运用solidworks和3D Studio Max软件对锁紧装置进行建模仿真.锁紧装置液压系统的设计主要采用比例阀控制的同步回路和用顺序阀的顺序动作回路来分别实现液压缸的同步运动以及顺序动作.     

基于MATLAB/SIMULINK的异步电动机软起动控制系统的仿真

针对异步电动机直接起动会产生很大起动电流的特性,提出了一种基于PID控制原理的异步电动机软起动控制方法。利用MATLAB/SIMULINK对此闭环控制系统建立了仿真模型并进行了仿真实验。仿真结果表明采用PID控制的软起动控制方法可以有效地限制异步电动机的起动电流,因此该控制方法是正确有效的。     

提高电力变压器抗短路能力的方法与措施

电力变压器是电力系统中关键的设备之一,阐述了电力变压器发生短路故障时产生过电流的危害性。通过对电力变压器绕组中短路电动力的分析,提出了电力变压器在设计、工艺、结构、选型和运行方面提高抗短路能力的方法与措施。     

杭州某高校学生公寓基坑施工监测分析

以浙江理工大学下沙校区学生公寓加建2号楼施工监测为例,掌握了该工程的深层土体水平位移、支撑轴力和地下水位的动态变化.监测表明:深层土体水平位移随深度变化可理解为"三角形"、"弓形",更准确地应理解为"右括弧形",其最大值发生在开挖后期的基坑开挖面附近;支撑轴力的变化可概括为四步曲等可借鉴的结论;地下水位的下降受季节性影响较大,在非雨季节水位下降呈基本线性变化,降雨天气水位有回升趋势.     

大涡模拟-Lighthill等效声源法的空腔水动噪声预测

为了考察空腔产生的水动噪声,对气动声学理论与研究方法进行了分析,确立了大涡模拟-等效声源混合法.利用大涡模拟计算空腔非定常流动的流场结构,基于有限元/无限元法利用变分形式的Lighthill声类比方程进行声场的求解.利用该混合法考察了雷诺数为1.2×105的空腔算例频域内噪声的辐射特性,基频值的预测结果与文献基本一致,低频纯音的远场辐射具备指向性,沿上游方向辐射能量较高,高频宽带噪音的传播不具指向性.     

Effect of Cooling Rate on the Solidification Behavior and Microstructure of SiC_w/Al-18Si Composites

Al-18Si alloy reinforced with 15%,20% and 25%(volume fraction) SiC whiskers were prepared by squeeze casting technique and the solidification behavior and microstructure of as-prepared composites at different cooling rates were studied by DSC,optical microscope,SEM and TEM.The results show that silicon phase is nucleated on SiC whiskers.With the increase of cooling rate,the degree of undercooling increases in the composites as well as in the alloys.The increase of cooling rate leads to a reduction in the size of eutectic Al-Si and also changes its morphology from short stick to equiaxed.However,the change of primary Si is complex.The primary Si size is refined,and then coarsened with increasing cooling rate.The primary Si morphology of composites changes from agglomerate to stick.     

大规模定制产品族协同优化设计中的设计管理

产品族设计是大规模定制中的核心内容,其基本思想是以产品平台战略为指导,针对细分市场中不同客户群的需求,进行产品平台和基于产品平台的相关系列产品设计,以低成本和快速开发周期满足客户的个性化需求。而作为连接设计与管理之间的桥梁、集设计与管理于一身的设计管理,众望所归,逐步凸显其重要性,成为企业发展的加速器。     

Evolution of carbides and carbon content in matrix of an ultra-high carbon sintered steel during heat treatment process

DTA, thermal expansion, XRD, and SEM were used to evaluate the effect of quenching temperature on the mechanical properties and microstructure of a novel sintered steel Fe-6Co-1Ni-5Cr-5Mo-1C. Lattice parameters and the mass fraction of carbon dissolved in the matrix of the steel quenched were investigated. It is discovered that the hardness of the steel increases with quench-ing temperature in the range of 840-900℃ and remains constant in the range of 900 to 1100℃. It decreases rapidly when the tem-perature is higher than 1100℃. The mass fraction of carbon dissolved in the matrix of the steel quenched at 840℃ is 0.38, but when the quenching temperature is increased to 1150℃, it increases to 0.98. The carbides formed during sintering are still present at grain boundaries and in the matrix of the steel quenched at low quenching temperatures, such as 840℃. When the quenching temperature is increased to 1150℃, most of the carbides at grain boundaries are dissolved with just a small amount of spherical M23C6 existing in the matrix of the quenched steel.