The hEag1 K+ Channel Inhibitor Astemizole Stimulates Ca2+ Deposition in SaOS-2 and MG-63 Osteosarcoma Cultures

The hEag1 (Kv10.1) K⁺ channel is normally found in the brain, but it is ectopically expressed in tumor cells, including osteosarcoma. Based on the pivotal role of ion channels in osteogenesis, we tested whether pharmacological modulation of hEag1 may affect osteogenic differentiation of osteosarcoma cell lines. Using molecular biology (RT-PCR), electrophysiology (patch-clamp) and pharmacology (astemizole sensitivity, IC₅₀ = 0.135 μM) we demonstrated that SaOS-2 osteosarcoma cells also express hEag1 channels. SaOS-2 cells also express to KCa1.1 K⁺ channels as shown by mRNA expression and paxilline sensitivity of the current. The inhibition of hEag1 (2 μM astemizole) or KCa1.1 (1 mM TEA) alone did not induce Ca²⁺ deposition in SaOS-2 cultures, however, these inhibitors, at identical concentrations, increased Ca²⁺ deposition evoked by the classical or pathological (inorganic phosphate, Pi) induction pathway without causing cytotoxicity, as reported by three completer assays (LDH release, MTT assay and SRB protein assay). We observed a similar effect of astemizole on Ca²⁺ deposition in MG-63 osteosarcoma cultures as well. We propose that the increase in the osteogenic stimuli-induced mineral matrix formation of osteosarcoma cell lines by inhibiting hEag1 may be a useful tool to drive terminal differentiation of osteosarcoma.     

Delay Asymptotics and Scalability for Peer-to-Peer Live Streaming

A large number of peer-to-peer streaming systems have been proposed and deployed in recent years. Yet, there is no clear understanding of how these systems scale and how multipath and multihop transmission, properties of all recent systems, affect the quality experienced by the peers. In this paper, we present an analytical study that considers the relationship between delay and loss for general overlays: we study the trade-off between the playback delay and the probability of missing a packet and we derive bounds on the scalability of the systems. We present an exact model of push-based overlays and show that the bounds hold under diverse conditions: in the presence of errors, under node churn, and when using forward error correction and various retransmission schemes.     

激光弯曲机理的评述

对激光弯曲变形机理问题进行了探讨,提出了激光弯曲的变形机理本质上是温度梯度机理,反向弯曲机理和增厚机理是当材料厚度方向的温度梯度很小时,针对不同厚度的材料而出现的现象.实验发现有限厚度材料在激光辐照作用下,其内部产生了用经典傅里叶定律无法解释的非常规温度分布,将其定义为泛傅里叶效应,用于进一步揭示激光弯曲机理的实质.     

The SBASE domain library: a collection of annotated protein segments

SBASE is a database of annotated protein domain sequences representingvarious structural, functional, ligand binding and topogenicsegments of proteins. The current release of SBASE contains27 211 entries which are provided with standardized names inorder to facilitate retrieval. SBASE is cross-referenced tothe major protein and nucleic acid databanks as well as to thePROSITE catalog of protein sequence patterns [Bairoch, A. (1992)Nucleic Acids Res., 20, Suppl., 2013–2118]. SBASE canbe used to establish domain homologies through database searchusing programs such as FASTA [Lipman and Pearson (1985) Science,227, 1436–1441], FASTDB [Brutlag et al. (1990) Comp. Appl.Biosci., 6, 237–245] or BLAST3 [Altschul and Lipman (1990)Proc. Natl. Acad. Sci. USA, 87, 5509–5513], which is especiallyuseful in the case of loosely defined domain types for whichefficient consensus patterns cannot be established. The useof SBASE is illustrated on the DNA binding protein Brain-4.The database and a set of search and retrieval tools are freelyavailable on request to the authors or by anonymous ‘ftp’file transfer from <ftp.icgeb.trieste.it>.     

Adverse Impact of Diet-Induced Hypercholesterolemia on Cardiovascular Tissue Homeostasis in a Rabbit Model: Time-Dependent Changes in Cardiac Parameters

The present study evaluates a hypothesis that diet-related hypercholesterolemia increases oxidative stress-related burden to cardiovascular tissue, resulting in progressively increased mortality, along with deterioration of electrophysiological and enzymatic function in rabbit myocardium. New Zealand white rabbits were divided into four groups, defined as follows: GROUP I, cholesterol-free rabbit chow for 12 weeks; GROUP II, cholesterol-free chow, 40 weeks; GROUP III, chow supplemented with 2% cholesterol, 12 weeks; GROUP IV, chow supplemented with 2% cholesterol, 40 weeks. At the 12 and 40 weeks time points, animals in each of the aforementioned cohorts were subjected to echocardiographic measurements, followed by sacrifice. Significant deterioration in major outcome variables measured in the present study were observed only in animals maintained for 40 weeks on 2% cholesterol-supplemented chow, with much lesser adverse effects noted in animals fed high cholesterol diets for only 12 weeks. It was observed that rabbits receiving high cholesterol diets for 40 weeks exhibited significantly increased mortality, worsened ejection fraction and general deterioration of cardiac functions, along with increased atherosclerotic plaque formation and infarct size. Additionally, myocardium of GROUP IV animals was observed to contain lower levels of heme oxygenase-1 (HO-1) and cytochrome c oxidase III (COX III) protein relative to the controls.     

薄板激光弯曲机理的研究

试件在激光与材料非熔凝作用下动态变形过程实时测量的结果表明：薄板在激光照射下所引起最终变形的方向取决于材料的性质，可能是朝向激光束或背向激光束。研究结果进一步完善了薄板激光弯曲的变形机理，并为研究薄板激光弯曲过程奠定了基础。