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991.
通过LPS(lipopolysaccharide)分别刺激树突状细胞和巨噬细胞,发现巨噬细胞中HtrA丝氨酸蛋白酶2(HtrA serine peptidase 2,htra2)基因的表达随着刺激时间的延长呈现下调的变化趋势,提示巨噬细胞中的htra2受到LPS的调控.为了进一步证实LPS刺激的特异性,实验建立VSV(vesicular stomatitis virus)病毒感染巨噬细胞模型,采用荧光定量PCR,免疫荧光方法分别从htra2的蛋白水平和定位上研究htra2的变化与LPS刺激巨噬细胞产生效应的相关性;构建了htra2基因过表达的质粒pKH3-htra2-HA,采用ELISA,Western Blot及荧光定量PCR等方法,检测过表达htra2对巨噬细胞产生和分泌炎症因子IL-6(Interleukin-6)及IL-1b(Interleukin-1b)的影响.实验结果表明:htra2仅在LPS刺激巨噬细胞的体系中受到调控,且过表达的htra2会上调巨噬细胞IL-1b和IL-6的表达水平. 相似文献
992.
Flotation tests,contact angle measurements,infrared spectrum analyses,X-ray analyses and computer simulation were carried out in order to study the activation mechanism of lime-depressed pyrite with ox... 相似文献
993.
网络自然属性与社会属性既相互联系又相互区别;大学生生活方式自然属性与社会属性也相互联系并相互区别;网络二重性对大学生生活方式二重性产生直接的影响。网络的自然属性重塑大学生的社会结构,二重地改变着大学生生活的时空结构。网络的社会属性削弱大学生现实社会关系的不平等性;改变大学生生活的社交范围,并为其提供更新的社交方式;二重地影响着大学生的思想与心理。 相似文献
994.
995.
某水电站引水线路通过一高阶地前缘滑坡体,前期勘察时对该滑坡稳定性进行过稳定分析,但未作有效支挡处理,渠道施工开挖2 a后发生滑动,对渠道造成严重破坏,并推迟发电日期。滑动后对滑坡体进行了勘察和稳定性分析,估算出支挡结构断面处的下滑推力,并提出治理建议,为滑坡治理设计提供了可靠依据。 相似文献
996.
The self-clocking principle (SCP) of Transmission Control Protocol (TCP) had been analyzed for a network implementing a per-flow buffering scheme. The ideal SCP is yet unknown for the Internet which implements a first-in–first-out buffering scheme. This paper derives an ideal SCP for the Internet by formulating the traffic transmission control as a typical control problem and then solving it by a control-theoretic approach. The ideal SCP reveals the defect of the SCP being deployed in the Internet that it is insufficient to avoid congestion by adjusting the packet effective window based on records of the outstanding packets of a single source; instead outstanding packets from other sources also have to be counted. The ideal SCP also reveals the difficulties of developing and implementing an effective self-clocking scheme for congestion control in the Internet. 相似文献
997.
Control of nanosystems with frictional dynamics using feedback control methods is important to a wide range of applications of nanotribology. This paper studies the tracking control problem of an array of nanoparticles moving on a substrate with friction between the substrate and the particles. The focus of this study is on control design and stability analysis. The major challenges in this problem include nonlinearities and uncertainties in the frictional dynamics and limited availability of measurable states in nanosystems. The particle–substrate interaction is considered to be unknown, and the unknown effect of unmodeled particle dynamics on the dynamics of the center of mass of the array is also considered. A nonlinear identifier is first developed to identify these unmodeled dynamics. A feedback controller is then developed based on the identifier to control the center of mass of the particles to track a desired trajectory. Boundedness of the closed-loop states and semiglobal asymptotic stability of the tracking error are proven using Lyapunov theory for the case of linear inter-particle interactions. An example with more general Morse-type inter-particle interactions is included to provide some level of confidence that the results are general but not assuredness that they are. Numerical simulation results are provided to demonstrate the performance of the developed identification and control law. 相似文献
998.
999.
An accurate closed form solution is proposed to estimate camera pose by several mirrored reference object images acquired via a planar mirror under different unknown poses. Compared with state-of-the-art methods, our method is more accurate when there are more than three images and has explicit geometric meanings. This method also properly handles cases in which some of the mirror poses are parallel. The central idea is to minimize an error metric based on all reflections of rotation, which enables the camera rotation to be estimated directly by SVD of sum of mirrored camera rotations. After that, the camera translation is computed by solving a large system of linear equations to minimize object space collinearity error. Both synthesized data and real data experiments show the advantages of our approach. 相似文献
1000.
Three-dimensional molecular dynamics(MD)simulation was carried out to understand the mechanism of water lubrication in nanometric cutting.The water-lubricated cutting was compared with the dry cutting process in terms of lattice deformation,cutting force,heat and pressure distribution,and machined surface integrity.It was found that water molecules effectively reduce the friction between the tool and workpiece,the heat in the cutting zone and the pressure being generated on the tool surface,thus leading to prolonged tool life.Water molecules also enlarged the pressure-affected area,which decreased the roughness of the machined surface. 相似文献