Studying the protein-protein interactions in the postsynaptic density by means of immunoabsorption and chemical crosslinking

Postsynaptic densities (PSDs), isolated from porcine cerebral cortices, are large disk-shaped aggregates consisting of hundreds of different proteins. To study the protein-protein interactions in such complex supramolecules, we developed a procedure to break up the PSD's overall structure, while preserving some interactions between individual proteins. Using the resulting PSD sample and an indirect immunoabsorption procedure, PSD-95 was isolated along with the α- and β-subunits of calcium calmodulin-dependent protein kinase II (CaMKIIα and CaMKIIβ), α-tubulin, β-tubulin, and Chapsyn110. Similarly, CaMKIIα was isolated along with CaMKIIβ, α-tubulin, β-tubulin, and small amounts of PSD-95. The proteins isolated from PSDs treated with a cleavable bifunctional crosslinking reagent were further subjected to diagonal gel electrophoresis analysis, and the results indicated that CaMKIIα resides next to α-tubulin in the PSD. Overall, the results obtained here suggest that within the PSD, large aggregates of CaMKIIα, CaMKIIβ, α-tubulin, and β-tubulin may occur that indirectly associate with PSD-95 and Chapsyn110. Such a protein organization would allow interactions with F-actin in the cytoplasm and with proteins, such as N-methyl-D-aspartate receptors, which reside on the postsynaptic membrane. Furthermore, it would facilitate binding to proteins such as the various microtubule-associated proteins that reside in the core region of the PSD.     

Performance evaluation of a dental handpiece in simulation of clinical finishing using a novel 2DOF in vitro apparatus

This paper reports on the performance evaluation of a dental handpiece in simulation of clinical finishing using a novel two-degrees-of-freedom (2DOF) in vitro apparatus. The instrumented apparatus consisted of a two-dimensional computer-controlled coordinate worktable carrying a dental handpiece, a piezoelectric force dynamometer, and a high-speed data acquisition and signal conditioning system for simulating the clinical operations and monitoring the dental finishing processes. The performance of the dental handpiece was experimentally evaluated with respect to rotational speed, torque, and specific finishing energy under the applied clinical finishing conditions. The results show that the rotational speeds of the dental handpiece decreased by increasing either the depth of cut or the feed rate at a constant clinically applied air pressure and water flowrate. They also decreased when increasing both the tangential and normal finishing forces. The specific finishing energy decreased with an increase in either depth of cut or feed rate, while the finishing torque increased as either the depth of cut or the feed rate was increased. Implications of these results were to provide guidance for proper applications of dental handpieces in clinical practice.     

WWW上路径模式发掘的研究

本文从互连网发展的趋势入手,对路径模式发掘进行了研究。先简要介绍数据库发掘的概况及网上数据发掘的特点,后结合关联法和组合概率法,并以算法和实例详细对之进行分析。     

谈公共设施中的环境陶艺形态

探讨公共设施环境陶艺形态的类型、表现手法及特点,指出公共设施中的环境陶艺形态设计要强调实用与审美、环境相统一。     

A structural model for the cholesterol-phosphatidylcholine complexes in bilayer membranes

Based on the structural properties of phospholipid and cholesterol molecules, and making use of the known structural and motional effects of cholesterol and its analogs on phospholipid bilayers, a model for the cholesterol-phosphatidylcholine complex is proposed. In this model, the 3β-hydroxyl group of cholesterol is assumed to engage in hydrogen bonding with the carbonyl oxygen of the fatty acyl groups in phospholipids. Some specific configurations of the saturated and unsaturated fatty acyl chains of the phospholipid are suggested to participate in van der Waals attractive interactions with the α and β surface of the steroid nucleus.     

SVD-based Preisach hysteresis identification and composite control of piezo actuators

In this paper, the singular value decomposition (SVD) based identification and compensation of the hysteretic phenomenon in piezo actuators are addressed using a Preisach model. First, this paper presents an SVD-based least squares algorithm and a revision approach of the identification through updating the SVD. With the identified parameters and a log of the memory curve, a Preisach-based inversion compensator is constructed which is complemented with a feedback controller to address the inevitable and residual modeling errors. Experimental results are furnished for both the identification and compensation approaches. The Preisach-based feedforward controller significantly improves the tracking performance and reduces the root-mean-square (RMS) tracking error of a PID controller by 76.7% and 89% at 1 Hz and 25 Hz, respectively. With the proposed composite controller, the percent-RMS errors at 1 Hz and 25 Hz are reduced to 0.035% and 0.31%, respectively.     

A private versus a public space: Anonymity and buying decorative symbolic goods for avatars in a virtual world

The virtual world has become a new channel in which people can contact with others, and organizations can provide service to their customers. Selling symbolic virtual goods has also emerged as a new economy in the virtual world. Such symbolic goods cannot facilitate people to accomplish any specific tasks; people buy them simply to decorate their avatars. This study is based on the theories of symbolic consumption, self-presentation, and computer-mediated environment, to investigate why people buy decorative symbolic goods in the virtual world. The results show that people buy symbolic virtual goods for both emotional and social values. While user perceived social presence and telepresence affect both social and emotional value of symbolic goods, individual self-presentation motivation affects only social value. Besides, anonymous users in the virtual world show behaviors that differ with non-anonymous ones.