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71.
Jackson PV Hunt JA Doherty PJ Cannon A Gilson P 《Journal of materials science. Materials in medicine》2004,15(4):507-511
Characterisation and quantification of the surface energy of biomaterials used as tissue engineering scaffolds is important, but many of the techniques available to examine these properties are only applicable to smooth flat samples, not porous materials. This paper describes the application of the Washburn equation to measure the surface energy of a range of porous polyether polyurethane scaffolds with three test liquids; n-Hexane was used to measure a material constant, whilst ethanol and xylene were used to measure contact angles. The results show that the Washburn equation is not applicable in its current form, reasons for this could be that the voids in the materials are too wide for effective capillarity; absorption of the solvents into the polymer matrix may further complicate the measured imbibition profile. Another possible reason is the differences between the sizes of the interconnecting pores in scaffolds with varying void sizes; this could affect the capillary effect of the test liquids through the material. The repeatability of the results and the similar patterns observed with the different liquids suggest that if these issues could be quantified and incorporated into the Washburn equation, it may be possible to generate useful results for similar materials. 相似文献
72.
Recent advances in phosphopeptide enrichment prior to mass spectrometric analysis show genuine promise for characterization of phosphoproteomes. Tandem mass spectrometry of phosphopeptide ions, using collision-activated dissociation (CAD), often produces product ions dominated by the neutral loss of phosphoric acid. Here we describe a novel method, termed Pseudo MS(n), for phosphopeptide ion dissociation in quadrupole ion trap mass spectrometers. The method induces collisional activation of product ions, those resulting from neutral loss(es) of phosphoric acid, following activation of the precursor ion. Thus, the principal neutral loss product ions are converted into a variety of structurally informative species. Since product ions from both the original precursor activation and all subsequent neutral loss product activations are simultaneously stored, the method generates a "composite" spectrum containing fragments derived from multiple precursors. In comparison to analysis by conventional MS/MS (CAD), Pseudo MS(n) shows improved phosphopeptide ion dissociation for 7 out of 10 synthetic phosphopeptides, as judged by an automated search algorithm (TurboSEQUEST). A similar overall improvement was observed upon application of Pseudo MS(n) to peptides generated by enzymatic digestion of a single phosphoprotein. Finally, when applied to a complex phosphopeptide mixture, several phosphopeptides mis-assigned by TurboSEQUEST under the conventional CAD approach were successfully identified after analysis by Pseudo MS(n). 相似文献
73.
Howard BJ Liland A Beresford NA Andersson KG Cox G Gil JM Hunt J Nisbet A Oughton DH Voigt G 《Radiation protection dosimetry》2004,109(1-2):63-67
The STRATEGY project (sustainable restoration and long-term management of contaminated rural, urban and industrial ecosystems; www.strategy-ec.org.uk) addressed the need for a holistic decision framework for the selection of optimal remediation strategies for long-term sustainable management of contaminated areas in Western Europe. The project considered both technical and social aspects of implementing restoration strategies for urban and rural environments. The importance of considering socially relevant objectives in addition to the dose reduction was emphasised. A critical evaluation was carried out on 101 selected countermeasures, (including rural waste disposal options), a model was developed to aid optimising countermeasure strategies and a method of carrying out participatory decision-making suggested. The outputs of the project are described and critically evaluated. 相似文献
74.
K.H. Hunt 《Mechanism and Machine Theory》1973,8(3):371-395
A study is here made of useful input-output motions from a class of profile-mechanisms characterised by a roller input driving a profiled follower. Both reciprocating and oscillating followers are considered. Compactness and favourable dynamic characteristicsare some of the advantages that this class of mechanism has over conventional cam mechanisms for certain forms of input-output motion which are broadly specified. 相似文献
75.
76.
This paper describes building standards relating to the fire code and their application to cold stores.Emphasis is placed on a policy for a large industrial complex with single management capable of ensuring permanent fire safety measures, emergency procedures and intensive staff training. The management can thus co-ordinate all aspects of fire prevention and decide which buildings should incorporate fire safety measures and the extent to which these should be implemented. Emphasis is especially laid on staff safety, specifying minimum egress distances and easy fire fighting access. The use of sprinklers, located selectively, is advised in some parts of the building.The fire code represents a consensus view of design engineers, fire fighters, local government, insurance companies and meat company owners and operators. 相似文献
77.
Optical tweezers are an important tool for studying cellular and molecular biomechanics. We present a robust optical tweezers device with advanced features including: multiple optical traps, acousto-optic trap steering, and back focal plane interferometry position detection. We integrate these features into an upright microscope, with no compromise to its capabilities (differential interference contrast microscopy, fluorescence microscopy, etc.). Acousto-optic deflectors (AODs) steer each beam and can create multiple time-shared traps. Position detection, force calibrations and AOD performance are presented. The system can detect subnanometer displacements and forces below 0.1 pN. 相似文献
78.
The inflammatory response resulting from the implantation of a medical device may compromise its performance and efficiency leading, in certain cases, to the failure of the implant. Thus, the assessment of the behavior of inflammatory cells in vitro, constitutes a key feature in the evaluation of the adverse potential, or not, of new promising biomaterials. The objectives of this study were to determine whether starch-based polymers and composites activated human neutrophils. Blends of starch with ethylene-vinyl alcohol, with cellulose acetate and polycaprolactone, as well as composites based on all these materials filled with hydroxyapatite have been studied. A lysozyme assay was adapted to examine enzyme secretion from human neutrophils incubated with different starch-based materials. Changes in the free radical and degranulation activity of the neutrophil were also determined by measuring the luminescent response of Pholasin®, a photoprotein that emits light after excitation by reactive oxygen species. The amount of lysozyme secreted by neutrophils incubated with the polymers did not exhibit significant differences between the tested materials. Results were in all cases similar to those obtained for the control (polypropylene) except for one of the starch blends (corn starch with polycaprolactone reinforced with 30% (w/w) of HA). The chemiluminescence experiments showed that polymers reduce the signal produced by activated neutrophils. Furthermore, for some polymers it was demonstrated that the phenomenon was due to an effect of the surface of the materials in cell adhesion or a simultaneous competition for the photoprotein in solution, which results in the decrease of the intensity of light emitted and detected. 相似文献
79.
Spatial representations of virtual mazes: the role of visual fidelity and individual differences 总被引:1,自引:0,他引:1
Twenty-four people learned three versions of a room-sized maze: a wire-frame desktop virtual environment (VE), a normal surface-rendered desktop VE, and a real-world maze. Differences among the mental representations formed from each environment were measured with pointing and distance estimation tasks in a real-world version of each maze. People were more accurate at pointing after having learned the real and wire-frame VE maze than the surface-rendered VE maze; however, this effect was small compared with the effect of individual differences. Differences in gender, spatial ability, and prior computer experience were significantly related to the ability to acquire spatial information from the desktop VE. There was a high correlation between spatial knowledge when it was measured in the VE and spatial knowledge measured in the real world. Actual or potential applications include the design of effective VE training systems. 相似文献
80.
This paper describes a nonlinear control structure known as a local controller network. The structure consists of a weighted combination of a number of individual controllers, each of which is valid locally in the state space of the plant. Local controller designs are based upon local models valid in operating regimes which do not necessarily contain any physical equilibria. Consequently, the transient performance can be improved. Some 'scheduling' variables determine the current operating regime, and a validity function is assigned to each local controller. A 'feedforward' component may be used in each local controller in order to compensate directly for the operating-point-dependent model offsets. The application of the local controller network approach to a nonlinear control problem, that of longitudinal vehicle dynamics control, is described. A stability analysis for the discrete-time local controller network is given in this paper and the results are compared with known theoretical guidelines for related control approaches such as gain scheduling and feedback linearization. 相似文献