Studies of substrate specificities of lipases from different sources

Although lipases are widely applied in a wide variety of reactions, available information on the factors that are responsible for the substrate specificities of lipases from different sources is scarce. In this paper, nine lipase‐producing microorganism strains were isolated from oil‐containing samples. The properties of these enzymes, including pH optima, temperature optima, thermal stability, and pH stability, vary significantly with the different sources from which these lipases were isolated. The substrate specificities of the nine lipases, including fatty acid and positional specificities, were also studied. The fatty acid specificities of the nine lipases were observably different toward 15 substrates with different carbon chain lengths, different saturation degrees and different side chains. The lipases from S₃ Penicillium citrinum (PCL), MJ₁ Aspergillus niger (ANL), MJ₂ Aspergillus oryzae (AOL), YM Bacillus coughing (BCL), S₉ Geotrichum candidum (GCL), and S₁₁ Candida lypolytica (CLL) showed the strongest specificities to short‐chain esters, and the other lipases showed strong selectivity for medium‐ or long‐chain and branched esters. The positional specificities were examined by analyzing the hydrolytic products of triolein catalyzed by the nine lipases, using TLC. The lipases can be mainly classified into two groups by their specificities for the ester bond at position 2 of triglycerides; one group can selectively hydrolyze the ester bond at position 2 of the triglycerides, the other group cannot. All these nine lipases were divided into four groups by hierarchical clustering analysis on the basis of the results of fatty acid and positional specificities.     

星型聚合物中间体环硅氧烷核的合成研究

以甲基二氯硅烷、四甲基四乙烯基环四硅氧烷(DVi4)为原料,采用硅氢加成反应合成了星型聚合物的中问体环硅氧烷核,并用红外光谱和核磁共振谱进行了表征.反应的最佳条件是:采用预先活化、反应温度72℃、催化荆与DVi4的量之比为1.0×10-4,反应转化率可达95%,且选择性很好,产物主要为β-加成产物.     

High-cycle fatigue characterization of titanium 5Al-2.5Sn alloy

High-cycle fatigue behaviour of titanium 5Al-2.5Sn alloy at room temperature has been studied. S-N curve characterization is performed at different stress ratios ranging from 0 to 0.9 on a subsized fatigue specimen. Both two-stress and three-stress level tests are conducted at different stress ratios to study the cumulative fatigue damage. Life prediction techniques of linear damage rule, double linear damage rule and damage curve approaches are applied, and results are compared with the experimental data. The agreement between prediction and experiment is found to be excellent.     

Specifying transformer winter and summer peak-load limits

This paper presents a methodology for specifying the winter and summer peak-load limits for substation transformers that carry a temperature-sensitive load, taking into account the random nature of load and ambient temperature as well as their correlation. With this methodology, we can easily and accurately specify the peak-load limits for each substation transformer once the historical ambient temperature and load data are available. Also, we can collectively specify the peak-load limits for the transformer of each cooling type (OA, OA/FA, or OA/FA/FA) in a geographic region based on the mean and standard deviation of ambient temperatures in that region. Such peak-load limits are useful for power system planning.     

用PIXE方法研究骨折家兔骨骼微量元素的变化

用质子激发X荧光分析法测定48只家兔在挠骨骨折愈合过程中骨骼中多种微量元素含量的变化。结果表明，Fe和Sr分别呈鼓凸形和下凹形规律性变化，故Fe和Sr含量的变化可作为骨折愈合过程的指示剂。在骨折愈合过程中发现骨骼和血液中元素钙的相对含量保持不变，故无法为西医用补充钙来治疗骨折的疗法提供依据，但我们的研究结果却表明中医补充微量元素治疗骨折的疗法是有道理的。     

Effects of EGb 761 on nitric oxide and oxygen free radicals, myocardial damage and arrhythmia in ischemia-reperfusion injury in vivo

The cardioprotective effects of EGb 761 on the release of nitric oxide (NO), the concentration of serum thiobarbituric acid reaction substance (TBARS), the activity of creatine kinase (CK) and the incidence of ventricular arrhythmias were investigated in myocardial ischemia-reperfusion injury in vivo. Using sodium nitrite (NaNO2) as standard source of nitric oxide (NO), we compared the correlation coefficients of the three measuring methods used currently in the determination of NOFe2+(DETC)2 complex with that of the measuring method suggested in this study. The result showed that measuring the whole height of three splitting signals is the best linear correlation to the concentration of NO comparing with other methods in this system. Using this method, we observed the effects of EGb 761 on NOFe2+(DETC)2 complex in myocardial ischemia-reperfusion injury in vivo. The hearts of the Wistar rats were subjected to 30 min of ischemia and 10 min of reperfusion in vivo. Different doses of EGb 761 (25, 50, 100, 200 mg/kg i.p.), superoxide dismutase (SOD, 10(4) U/kg), l-arginine (50 mg/kg i.p.) and nitric oxide synthase (NOS) inhibitor NG-nitro-l-arginine (NNA, 50 mg/kg i.p.) were administered to the ischemia-reperfusion rats. EGb 761 under the dose of 100 mg/kg increased the signal intensity of NOFe2+(DETC)2 complex, while EGb 761 at 200 mg/kg showed an effect of decreasing the signal intensity of NOFe2+(DETC)2 complex. EGb 761 inhibited the formation of TBARS, the release of CK, and mitigated the incidence of ventricular arrhythmias in a dose dependent way. Both l-arginine and SOD increased the signal intensity of NOFe2+(DETC)2 complex and inhibited the formation of TBARS, the leakage of CK and the incidence of ventricular arrhythmia. NNA not only had no protective effects on myocardial injury, but also increased the incidence of reperfusion-induced arrhythmia. In conclusion, EGb 761 has cardiovascular protective effects by means of adjusting the level of NO and inhibiting oxygen free radicals induced lipid peroxidation in myocardial ischemia-reperfusion injury in vivo.     

Authority updating: An expert authority evaluation algorithm considering post‐evaluation and power indices in social networks

In group assessment, the focus is on finding high‐authority experts to improve the reliability of assessment results. In this study, we propose an authority updating algorithm while considering the power and judgement reliability of an expert on the basis of social networks and post‐evaluations. A network power index is established and used to reflect the power of an expert while considering social networks. The measurement of the judgement reliability of an expert considers the post‐evaluation of the objects selected by experts, thereby more scientifically reflecting the reliability of experts. The analysis shows the following: although the social‐network structure influences the authority of experts, the influence weakens when the assessment group is a highly or even fully connected group; the network effect may increase the authority of some experts and reduce that of others, and it will weaken as the network connectivity increases; moreover, the judgement reliability and authority of an expert while considering post‐evaluation can encourage him/her to make fair assessments and strive to reduce his/her motivation and cognitive biases.     

对《网络创业实训》课程的探讨

针对各校对大学生创业实训进行讲授内容的不同,我们在结合本专业的基础上,开展了网络创业实训课程,在创业方向方面不再仅限于网店,而是可以进行多种模式的选择,同时在提高实践性方向,最终的成果也以创业企划书来全面衡量。     

热压变形对灰口铸铁石墨形态的影响

研究了灰口铸铁(HTl50)在900℃大压下量塑性变形后石墨的分布形态，结果表明：随着压下量的增加，石墨片逐渐趋于平行分布；变形过程中发生了碳的回溶，同时伴随着石墨片的断裂；石墨片体积分数及片间距随压下量的增加而逐渐减少。