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Sara De Vincentiis Matteo Baggiani Francesca Merighi Valentina Cappello Jakub Lopane Mariachiara Di Caprio Mario Costa Marco Mainardi Marco Onorati Vittoria Raffa 《Small (Weinheim an der Bergstrasse, Germany)》2023,19(30):2205871
Mechanical stimulation modulates neural development and neuronal activity. In a previous study, magnetic “nano-pulling” is proposed as a tool to generate active forces. By loading neural cells with magnetic nanoparticles (MNPs), a precise force vector is remotely generated through static magnetic fields. In the present study, human neural stem cells (NSCs) are subjected to a standard differentiation protocol, in the presence or absence of nano-pulling. Under mechanical stimulation, an increase in the length of the neural processes which showed an enrichment in microtubules, endoplasmic reticulum, and mitochondria is found. A stimulation lasting up to 82 days induces a strong remodeling at the level of synapse density and a re-organization of the neuronal network, halving the time required for the maturation of neural precursors into neurons. The MNP-loaded NSCs are then transplanted into mouse spinal cord organotypic slices, demonstrating that nano-pulling stimulates the elongation of the NSC processes and modulates their orientation even in an ex vivo model. Thus, it is shown that active mechanical stimuli can guide the outgrowth of NSCs transplanted into the spinal cord tissue. The findings suggest that mechanical forces play an important role in neuronal maturation which could be applied in regenerative medicine. 相似文献
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Holzer Jakub Husťák Miroslav Hegrová Jitka Gröger Roman 《Metallurgical and Materials Transactions A》2023,54(2):439-449
Metallurgical and Materials Transactions A - Experimental studies on single crystals of pure metals are essential for understanding the mechanisms governing their plastic deformation as well as for... 相似文献
525.
Mays S. Q. Al-Fakhar Nurgül Bilgin Laust Moesgaard Apolonia Witecka Prof. Dr. Jakub Drozak Prof. Dr. Jacob Kongsted Prof. Dr. Jasmin Mecinović 《Chembiochem : a European journal of chemical biology》2023,24(21):e202300490
Nτ-methylation of His73 in actin by histidine methyltransferase SETD3 plays an important role in stabilising actin filaments in eukaryotes. Mutations in actin and overexpression of SETD3 have been related to human diseases, including cancer. Here, we investigated the importance of Trp79 in β-actin on productive human SETD3 catalysis. Substitution of Trp79 in β-actin peptides by its chemically diverse analogues reveals that the hydrophobic Trp79 binding pocket modulates the catalytic activity of SETD3, and that retaining a bulky and hydrophobic amino acid at position 79 is important for efficient His73 methylation by SETD3. Molecular dynamics simulations show that the Trp79 binding pocket of SETD3 is ideally shaped to accommodate large and hydrophobic Trp79, contributing to the favourable release of water molecules upon binding. Our results demonstrate that the distant Trp79 binding site plays an important role in efficient SETD3 catalysis, contributing to the identification of new SETD3 substrates and the development of chemical probes targeting the biomedically important SETD3. 相似文献