Design,refinement, implementation and prototype testing of a reconfigurable lathe-mill

This article documents the design, refinement, and implementation of a reconfigurable machine tool that provides a flexible platform for turning and milling. Advances in the design and capabilities of machine tools drive modern industry. In fact, the expanding capabilities of the machines permit novel and economic changes in the manufacturing and design of products. After the flexible manufacturing (FMS) boom, a more recent, but no less significant, area of machine development involves the reconfiguration of milling machines. This reconfigurability can also be applied to lathe-mills. The present work demonstrates satisfactorily the reconfiguration characteristics of modularity, integrability, and convertibility. We prototype and test a completely functional bench top reconfigurable lathe mill machine implemented using a $10,000 USD budget and developed following a synthesized machine tool product development methodology that begins with concept design and cutting forces to end in prototyping. In addition to reasonable results in runout, positioning, and surface roughness, the machine demonstrates the capability to produce parts with a Cpk of 1.009, thereby demonstrating the ability to fabricate this type of machine in Mexico for the local jewelry industry.     

Numerical experience with diagonalization algorithms for asymmetric demand traffic assignment

This paper reviews the concept of a diagonalization algorithm for use in solving traffic network equilibrium problems for which the arc cost and/or the origin-destination travel demand functions are asymmetric. Such functions are known to occur in realistic settings involving multiple modes or users. The computational performance of this algorithm for different degrees of travel demand asymmetry is then explored by a detailed numerical experiment since no previous results of this type have been reported. It is found that, through the use of progressive stopping tolerances, the impact of high degrees of travel demand function asymmetry on the computational burden associated with finding a traffic network equilibrium may be mitigated; in effect equilibrium problems with high degrees of demand asymmetry are little more difficult to solve than perfectly symmetric problems.     

Identification and Structure–Activity Relationship Studies of Small‐Molecule Inhibitors of the Methyllysine Reader Protein Spindlin1

The methyllysine reader protein Spindlin1 has been implicated in the tumorigenesis of several types of cancer and may be an attractive novel therapeutic target. Small‐molecule inhibitors of Spindlin1 should be valuable as chemical probes as well as potential new therapeutics. We applied an iterative virtual screening campaign, encompassing structure‐ and ligand‐based approaches, to identify potential Spindlin1 inhibitors from databases of commercially available compounds. Our in silico studies coupled with in vitro testing were successful in identifying novel Spindlin1 inhibitors. Several 4‐aminoquinazoline and quinazolinethione derivatives were among the active hit compounds, which indicated that these scaffolds represent promising lead structures for the development of Spindlin1 inhibitors. Subsequent lead optimization studies were hence carried out, and numerous derivatives of both lead scaffolds were synthesized. This resulted in the discovery of novel inhibitors of Spindlin1 and helped explore the structure–activity relationships of these inhibitor series.     

Production and purification of a fused recombinant protein gp-36 (HIV-2) from Escherichia coli

A fragment of the gp-36 gene of the Human Immunodeficiency Virus type 2 (HIV-2) was fused to a stabilizer sequence, which encodes for the first N-terminal 58 amino acids of the human interleukin-2. The fused protein was expressed under the control of the tryptophan promoter in Escherichia coli, and expressed as 20% of the total cellular protein. Transmission electron microscopy indicated that the fusion protein formed cytoplasmic insoluble inclusion bodies. Inclusion bodies were semipurified by a wash pellet cell procedure, rendering a material with a purity higher than 70% by SDS–polyacrylamide gel electrophoresis. After solubilization with urea, this preparation was further purified by gel-filtration chromatography up to 95% purity.     

Note: Autoignition Temperature of Selected Polymers at Elevated Oxygen Pressure and Their Heat of Combustion

Autoignition temperature and heat of combustion are two important parameters in determining the oxygen compatibility of materials. This study investigates the autoignition temperature of 32 polymers at an elevated oxygen pressure of 10.3 MPa and reports their heat of combustion. © 1996 by John Wiley & Sons, Ltd.     

An investigation of scotopic threshold-versus-illuminance curves for the analysis of color-matching data

Stiles developed a method for estimating whether rod-photoreceptor activity contributed to color-matching data. This method requires knowledge of the rod threshold at the illuminance levels of lights being viewed. Stiles suggested that the Aguilar and Stiles scotopic threshold-versus-illuminance (TVI) curve could serve as a convenient approximation of the rod threshold. Stiles' method was intended for use in color-matching experiments where the stimuli are presented side by side, but, in the Aguilar and Stiles experiment, detection thresholds were measured for temporally pulsed lights. This study compares rod-sensitivity with detection and side-by-side adjustment tasks. The illumination level of the test and background light was kept below the absolute threshold of the short-wavelength sensitive cones, because there is evidence that cone signals may interact with the scotopic TVI function. The threshold data fell mainly within the range of the Aguilar and Stiles observers. There were two notable deviations from the Aguilar and Stiles curve: (1) consistent with previous reports, the scotopic TVI function had a slope shallower than the Aguilar and Stiles curve when measured with a short-wavelength background; (2) thresholds measured from a dark surround with a spatial adjustment task were higher than the Aguilar and Stiles thresholds even though the slopes were the same. © 1996 John Wiley & Sons, Inc.