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41.
Kyogoku K Yoshida K Watanabe H Yamashita T Kawabe Y Motono M Nishijima K Kamihira M Iijima S 《Journal of Bioscience and Bioengineering》2008,105(5):454-459
We previously reported the production of recombinant proteins using genetically manipulated chickens and quails. In this study, we constructed a retroviral vector encoding an expression cassette for a fusion protein of the extracellular domain of the human tumor necrosis factor (TNF) receptor 2 and Fc region of human IgG1 (TNFR/Fc), which is expected as an effective drug for inflammatory diseases such as rheumatoid arthritis. The concentrated viral vector was injected into developing chicken embryos. The chickens that hatched stably produced TNFR/Fc in the serum and egg yolk for six months. It appears that the fused protein is transported and accumulated into yolk from the serum, which is mediated by the Fc receptor. The protein purified from the yolk and serum inhibited the cytotoxic activity of TNF-* toward L929 cells, indicating that the protein produced by the chickens is biologically active. These results indicate the effectiveness of the recovery of Fc-fused proteins from the yolk of genetically manipulated chickens. 相似文献
42.
Yasunori Hamauzu Toshiya Nosaka Fuyu Ito Takanori Suzuki Shuichi Torisu Miyoko Hashida Akira Fukuzawa Masakatsu Ohguchi Shigeru Yamanaka 《Food chemistry》2011
Rapidly dried onion (Allium cepa L. cv. Momiji No. 3) powder (OP) prepared from the outer layers (from second to fourth scale leaves from the surface) of onion bulbs was analysed for its quercetin and polyuronide contents, the effects of enzymatic treatment and the anti-atherogenic effect on rats fed a high-fat diet. Quercetin 4′-glucoside (50%), free quercetin (30%) and quercetin 3,4′-diglucoside (20%) were identified as quercetin derivatives, and boiling-water extraction was effective in extracting these compounds. OP contained 12.9% of polyuronides, the basic skeleton of pectin. Enzymatic degradation (cellulase and pectinase, 50 °C for 12 h, pH 6.0) of OP was effective in obtaining a slurry of smaller particle sizes. The free quercetin increased and the glucosides decreased with enzyme treatment. In Wistar rats fed an OP-added high-fat diet, the total cholesterol, HDL-cholesterol and triglyceride concentrations were not significantly different from the rats fed a high-fat diet without OP. However, the atherogenic index (AI) of Wistar rats fed an OP-added high-fat diet was lower (AI = 3.3) than rats fed the diet without OP (AI = 4.1). The incremental elastic modulus (IEM) of the aorta from rats fed the OP-added diet was also significantly lower than that of the rats fed the diet without OP. The AI and IEM values of the rats fed the OP-added diet were quite similar to the values of rats fed the diet without OP but were allowed spontaneous exercise. These results suggest that OP intake is effective for decreasing the risk of arteriosclerosis. 相似文献
43.
Chlorine Gas Sensing Properties of ZnO-CaO Ceramics 总被引:2,自引:0,他引:2
The chlorine gas sensing properties of pure ZnO (PZ) and CaO-added ZnO (CAZ) ceramics with relative densities of 48–65% were investigated from 25–380°C in Cl2 concentration range from 0.9 ppm to 20 ppm using air or N2 as a carrier gas. In the entire temperature range, the conductivity of PZ and CAZ ceramics decreased by introducing Cl2 gas. 3 mol% CAZ showed a Cl2 sensitivity ((0)/(Cl2)) of 10 for 0.9 ppm Cl2 in air at 300°C, suggesting its usefulness for sensing of a low-concentration Cl2 gas below 1 ppm. The CaO addition was found to decrease the Cl2 sensitivity but to improve the recovery process after removing Cl2 gas. These effects are attributed to changes in the amount and stability of adsorbed oxygen and to subsequent changes in Cl2 adsorption behavior. 相似文献
44.
The gaseous products generated by the flaming combustion of ten kinds of synthetic polymers and a kind of wood (cedar) under the same conditions (sample weight, 0.1 g; temperature, 700°C air flow rates, 50 and 100 l./hr) were quantitatively analyzed by infrared spectrophotometry, gas chromatography, and colorimetric tube method. The main hydrocarbons generated were methane, ethylene, and acetylene. The amount of acetylene generated by the flaming combustion of polymers was much larger than the amount of acetylene formed by pyrolysis at 700°C in nitrogen. Acetylene increased in quantity with increasing air. For nitrogen compounds, hydrogen cyanide was generated from every polymer containing nitrogen used, but ammonia was detected only for nylon 66 and polyacrylamide. Nitrogen monoxide and nitrogen dioxide were detected only in small amounts. Nitrous oxide was detected in the gaseous products generated by the nonflaming combustion of urea resin and melamin resin. It was also found that about 70% of the nitrogen in N-66 and PAA was converted into nitrogen gas (N2) by combustion under the conditions described above. 相似文献
45.
Ferritin nanoparticles ornamented with a Ti-binding peptide are versatile nanoscaled building blocks. Their specific binding ability is strong enough to position them on nanopatterned Ti regions on a Pt substrate. Furthermore, the peptides mineralization activity enables the formation of titania on the outer side of the particle, and the particle's inner nanospaces can serve as a carrier for inorganic nanodots. Making use of all these properties, here we show controlled in aqua fabrication of three-dimensional nanoscale structures. The X-Y positioning obeyed the specific binding of the peptide, while fabrication in the Z-dimension entailed stepwise formation of titania and ferritin layers by alternately applying the binding and mineralization abilities of the Ti-binding peptide. This method paves the way for in aqua fabrication of nanodevices having complicated structures and functions. 相似文献
46.
Ken-Ichi Kusumoto Akihiro Hino Yuichi Yonekura Yoichi Nogata Hideaki Ohta Hiroyuki Takano 《Yeast (Chichester, England)》1998,14(6):583-586
Homologs of Saccharomyces cerevisiae STE3, a -factor receptor gene were detected from S. exiguus NFRI 3539 by low stringency Southern hybridization. This strain might have at least two types of homolog. One of these homologs, designated as e-STE3 was cloned. Its nucleotide sequence revealed 60% identity to STE3. The putative protein coding region consisted of 453 amino acid residues. The amino acid sequence identity between STE3 and e-STE3 was 62%, and that of the N-terminal 303 amino acid residues considered to be the pheromone binding domain was 79%. The e-STE3 sequence submitted to the DDBJ/EMBL/GenBank data libraries is available under Accession Number AB003086. © 1998 John Wiley & Sons, Ltd. 相似文献
47.
Kazuya Ohmatsu Hiroyasu Yumura Ken-Ichi Takahashi Ken-Ichi Sato Takeshi Ueda Yoshinobu Suganuma 《Electrical Engineering in Japan》1997,120(2):8-18
Superconducting ac machines such as transformers and reactors are expected to have an important role in future electric power transport lines. In these machines, superconducting coils are wound with superconducting cables that have low ac loss, stable ac quenching current, and high normal resistivity. We have developed Nb-Ti superconducting cables with ultrafine filaments and high-resistivity matrix for these coils. One such cable is a double-stranded round structure using 0.2-mm strands with 0.14-μm filaments and Cu-30wt%Ni as a matrix material. The 50-Hz quenching current without external magnetic field exceeds 1400 Arms. The ac loss is 15 kW/m3 at a transverse external magnetic field of 0.5 T, 50 Hz, and the normal resistivity is 0.21 Ω/m at 0 T, 10 K. © 1997 Scripta Technica, Inc. Electr Eng Jpn, 120(2): 8–18, 1997 相似文献
48.
Kawabe Y Naka T Ando-Noumi N Matsumoto H Ono K Nishijima K Kamihira M Iijima S 《Journal of Bioscience and Bioengineering》2006,102(6):518-523
We examined the transport of human immunoglobulin G (IgG) subclasses and fusion proteins with the Fc region of human IgG to the egg yolk, after the proteins were injected into a vein of hens. Human IgGs were efficiently transported and accumulated into the yolk, whereas the proteins were not detected in the egg white. Among human IgG subclasses, IgG2 was transported most efficiently. Fc-fusion proteins injected were also transported into the yolk. A fusion protein with the Fc region derived from human IgG2 was more efficiently transported into the yolk than the counterpart fusion with the Fc region from human IgG1. This study shows that the recovery of recombinant antibodies and Fc-fusion proteins from the yolk is an effective method in transgenic chicken bioreactors. 相似文献
49.
Mimura K Dang F Kato K Imai H Wada S Haneda H Kuwabara M 《Journal of nanoscience and nanotechnology》2012,12(5):3853-3861
Ordered structures of barium titanate (BT) nanocubes, strontium titanate (ST) nanocubes and BT/ST nanocubes mixture were directly fabricated on Si and Pt-coated Si substrates using a capillary force assisted assembly method. The morphology of self-assembled structures was observed using field emission scanning electron microscopy (FE-SEM) and scanning probe microscopy (SPM). It was revealed that nanocubes were arranged with various degrees of ordering to develop multilayer and monolayer regions at the surface of substrates. The elemental mapping of the structure consisting of the nanocubes mixture was also investigated by transmission electron microscopy (TEM) with an energy dispersive X-ray spectroscopy (EDX). It was revealed that BT and ST nanocubes coexisted homogeneously in the structure and had possibility to be arranged in order to each other. The piezoresponse properties obtained by scanning probe microscopy (SPM) indicated that the hetero-interface between BT and ST nanocubes would introduce anomaly in piezoelectric properties. 相似文献
50.
Yokoi KJ Shinohara M Kawahigashi N Nakagawa K Kawasaki K Nakamura S Taketo A Kodaira K 《International journal of food microbiology》2005,99(3):297-308
Shotgun cloning of the Lactobacillus gasseri JCM 1131T whole DNA yielded two recombinant plasmids, p118gaY1 and p118gaY2, which directed cell lysis activity. Sequencing analysis revealed that the two plasmids carried almost identical inserted genes in following orders (truncated genes, in parentheses): in p118gaY1, (orf149)-orf92-holgaY-lysgaY-orf35-attL-(mnaAgaY1); in p118gaY2, (orfXgaY1)-orf169-orf149-orf92-holgaY-lysgaY-orf35-attP-(intgaY). The lysgaY-encoded protein (designated as LysgaY, 33.7 kDa) showed significant homology with putative muramidases (peptidoglycan-degrading enzyme) of the Lactobacillus phage phiadh, Lj965, Lj928, LL-H, mv4, and mv1. By zymogram analysis, LysgaY overproduced in Escherichia coli exhibited lytic activity towards 17 Gram-positive bacterial strains, including lactobacilli, lactococci, and staphylococci. The holgaY-encoded protein (15.7 kDa) contained three potential transmembrane helices, resembling putative holins (cytoplasmic membrane-disrupting protein) of Lj928 and Lj965. On the other hand, another clone p118gaYR obtained by EcoRI-shotgun cloning carried the (ptsCgaY1)-attR-(intgaY) genes. Three sequences, attL, attP, and attR, had a 47-bp common (core) sequence, and the core of attR was located in 3' region of a potential tRNA(Arg) gene. These results suggested that (i) attL and attR are phage-host junctions, left- and right-arms, respectively, (ii) attP is a phage attachment site, and (iii) intgaY is an integrase gene for phage integration and/or excision. After mitomycin C-induction, phage particles were demonstrated by electron microscopy. The prophage (phigaY) is somewhat leaky in the host, and has the two-component lysis system (HolgaY-LysgaY), closely resembling that of Lj928 as well as Lj965. 相似文献