防水墙技术初探

防水墙技术是一种防止内部信息向外扩散的技术。论文给出了防水墙的定义及功能特性,并通过对防水墙系统WaterBox的介绍分析,探讨了利用防水墙技术构筑内网信息安全的可行性及发展趋势。     

对称配筋小偏心受压构件的截面设计

对小偏心受压构件在对称配筋情况下的截面配筋计算，提出用最佳一次逼近多项式简化基本公式，直接按二次方程求解ξ的近似方法，具有精度高，相对误差小，计算方便等优点，可供工程设计人员参考。     

大窝山隧道进口围岩快速掘进施工组织与管理

结合工程概况及工程特点,从机械设备配置、劳动力组织、现场管理等方面介绍了大窝山隧道进口Ⅱ级,Ⅲ级围岩段的快速掘进施工组织与管理措施,为类似隧道的快速掘进施工组织与管理提拱了相关经验.     

PURIFICATION AND PROPERTIES OF TRANSGLUTAMINASE FROM STREPTOVERTICILLIUM MOBARAENSE

Transglutaminase (TGase) was separated from the culture broth of an isolated strain of Streptoverticillium mobaraense. The crude enzyme was prepared by centrifugation, ultrafiltration, precipitation by alcohol, centrifugation and freeze‐drying. The yield after these processes was 65–70%. Then the enzyme was purified to homogeneity by chromatography on CM‐cellulose and Sephadex G‐75 on which the yields were about 70% and 80%, respectively; the purified folds reached 2.5–4.7 and 1.08–2.06, respectively. The molecular weight of this TGase was 39,500–40,100 Da by gel filtration chromatography. Optimum enzyme activity was observed in the pH range of 5.0–7.0, and it was maintained stable at 20–40C. The optimal temperature and pH was 52C and 6.0, respectively. At 1 mM and 5 mM metal ion or inhibitors concentration, TGase activity was strongly inhibited by Zn²⁺ and NEM, and not affected obviously by Ba²⁺, Ca²⁺, Co²⁺, Cu²⁺, Fe²⁺, Fe³⁺, Mg²⁺, Mn²⁺, Na⁺ as well as PMSF and EDTA. The effects of these additions on this TGase were compared with those of other microbial TGases.     

固有频率在车轮设计有限元分析中的应用

以三维软件进行车轮造型,用CAD软件建立固有频率分析数学模型,对车轮固有频率进行分析和处理.结合其他试验的分析,对车轮进行优化设计.     

基于m法线性有限元分析在桩基计算中的应用

针对目前各类有限元软件在工程设计中的应用已相当普遍,提出了通过有限元模型解决桩基的强度验算和水平位移的计算,不仅使桩的设计变得简单,而且还可在相当的精度内考虑桩基和上部结构的共同作用。     

浅谈南水北调中线S13标渠道机械化衬砌施工技术

南水北调渠道混凝土衬砌,由于其工程等级属于Ⅰ等,总干渠渠道为Ⅰ级建筑物,且渠底宽度较大,边坡坡度较缓,衬砌分缝较多,无论从资源投入、工效还是工程质量达到设计要求都有相当大的难度,渠道衬砌机施工技术正好解决这个难题,实现了机械化作业,值得在南水北调中线工程渠道施工中推广和应用.     

浅谈GPS在矿山测量中的应用

根据GPS的使用现状,结合测绘工作的一些感悟和经验,简单介绍GPS RTK(Real Time Kine-matic)工作原理及其在矿山测量、道路测量等实际工作的作业方法和注意的问题,及GPS在应用过程中的一些体会。