Identification and Validation of a Discrete Element Model for Concrete

The use of a three-dimensional discrete element method (DEM) is proposed to study concrete structures submitted to dynamic loading. The aim of this paper is to validate the model first in the quasistatic domain, and second in dynamic compression, at the sample scale. A particular growing technique is used to set a densely packed assembly of arbitrarily sized spherical particles interacting together, representing concrete. An important difference from classical DEMs where only contact interactions are considered, is the use of an interaction range. First, the correct identification of parameters of the DEM model to simulate elastic and nonlinear deformation including damage and rupture is made through quasistatic uniaxial compression and tension tests. The influence of the packing is shown. The model produces a quantitative match of strength and deformation characteristics of concrete in terms of Young’s modulus, Poisson’s coefficient, and compressive and tensile strengths. Then, its validity is extended through dynamic tests. The simulations exhibit complex macroscopic behaviors of concrete, such as strain softening, fractures that arise from extensive microcracking throughout the assembly, and strain rate dependency.     

Development and Preliminary Validation of the Physiological Hyperarousal Scale for Children.

Considerable empirical support exists for the positive affect and negative affect components of the tripartite model of anxiety and depression proposed by L. A. Clark and D. Watson (1991); however, less attention has been paid to the physiological hyperarousal component of the model. The development of the Physiological Hyperarousal Scale for Children (PH-C; J. Laurent, S. J. Catanzaro, & T. E. Joiner Jr., 1995) is described. The psychometric properties of items are examined using students in Grades 6-12 (N = 398). Initial scale validation includes a joint factor analysis with the Positive and Negative Affect Scale for Children (PANAS-C; J. Laurent et al., 1999; J. Laurent, K. Potter, & S. J. Catanzaro, 1994). The relationship between the PH-C and existing measures that tap related constructs is examined. Together, the PH-C and PANAS-C provide a means to assess tripartite model constructs useful in differentiating anxiety and depression. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Towards Pseudonymous e-Commerce

The lack of privacy is one of the main reasons that limits trust in e-commerce. Current e-commerce practice enforces a customer to disclose her identity to the e-shop and the use of credit cards makes it straightforward for an e-shop to know the real identity of its customers. Although there are some payment systems based on untraceable tokens, they are not as widely used as credit cards. Furthermore, even without buying anything, a customer is already disclosing some information about who or where she may be by just connecting to the e-shop's web server and leaving behind an IP-address. In this paper, we present novel components that enable secure pseudonymous e-commerce. On the one hand, these components allow a customer to browse through an e-shop, select goods, and pay the goods with her credit card such that neither the e-shop operator nor the credit card issuer nor an eavesdropper is able to get any information about the customer's identity. On the other hand, it is guaranteed that none of the involved parties is able to act dishonestly during the credit card payment. Such a system could greatly enhance trust in e-commerce since it overcomes the customers' privacy concerns.     

Energy Partitions and Image Segmentation

We address the issue of low-level segmentation for real-valued images. The proposed approach relies on the formulation of the problem in terms of an energy partition of the image domain. In this framework, an energy is defined by measuring a pseudo-metric distance to a source point. Thus, the choice of an energy and a set of sources determines a tessellation of the domain. Each energy acts on the image at a different level of analysis; through the study of two types of energies, two stages of the segmentation process are addressed. The first energy considered, the path variation, belongs to the class of energies determined by minimal paths. Its application as a pre-segmentation method is proposed. In the second part, where the energy is induced by a ultrametric, the construction of hierarchical representations of the image is discussed.     

Distributed machining control and monitoring using smart sensors/actuators

The study of smart sensors and actuators led, during the past few years, to the development of facilities which improve traditional sensors and actuators in a necessary way to automate production systems. In another context, many studies have been carried out aimed at defining a decisional structure for production activity control and the increasing need of reactivity leads to the autonomization of decisional levels close to the operational system. We study in this paper the natural convergence between these two approaches and we propose an integration architecture, dealing with machine tool and machining control, that enables the exploitation of distributed smart sensors and actuators in the decisional system.     

Correlated fluorescence lifetime and spectral measurements in living cells

Studies of proteins' interaction in cells by FRET can take benefit from two important photo-physical properties describing fluorescent proteins: fluorescence emission spectrum and fluorescence lifetime. These properties provide specific and complementary information about the tagged proteins and their environment. However, none of them taken individually can completely quantify the involved fluorophore characteristics due to their multiparametric dependency with molecular environment, experimental conditions, and interpretation complexity. A solution to get a better understanding of the biological process implied at the cellular level is to combine the spectral and temporal fluorescence data acquired simultaneously at every cell region under investigation. We present the SLiM-SPRC160, an original temporal/spectral acquisition system for simultaneous lifetime measurements in 16 spectral channels directly attached to the descanned port of a confocal microscope with two-photon excitation. It features improved light throughput, enabling low-level excitation and minimum invasivity in living cells studies. To guarantee a fairly good level of accuracy and reproducibility in the measurements of fluorescence lifetime and spectra on living cells, we propose a rigorous protocol for running experiments with this new equipment that preserves cell viability. The usefulness of SLiM approach for the precise determination of overlapping fluorophores is illustrated with the study of known solutions of rhodamine. Then, we describe reliable FRET experiments in imaging mode realized in living cells using this protocol. We also demonstrate the benefit of localized fluorescence spectrum-lifetime acquisitions for the dynamic study of fluorescent proteins. proteins.     

Methane calibration burner and C‐factor determination with cone calorimeter – validation of the standardized calibration protocol

The cone calorimeter is one of the major fire tests. In its reference standard ISO 5660‐1, the apparatus (and especially a characteristic of its orifice plate mass flow meter, called C‐Factor) is calibrated using a methane burner set at 5 kW. However, measurements performed in end‐use are in the range 1–10 kW. In addition, standard proposes to check linearity at 1 and 3 kW, which means in the lowest part of the range. This communication establishes the validation of the linearity on the whole range and determines the optimal condition for C‐factor determination. It justifies the technical choices of the standard. Copyright © 2014 John Wiley & Sons, Ltd.     

Poly(L‐lactic acid) with added α‐tocopherol and resveratrol: optical,physical, thermal and mechanical properties

Poly(L ‐lactic acid) (PLLA) films containing various concentrations of two natural antioxidants, α‐tocopherol and resveratrol, were fabricated by a melt compounding and compression molding process. The influence of the antioxidants on the optical properties such as color and UV‐visible light transmission was analyzed. The thermal, mechanical, rheological and physical properties of PLLA films with added antioxidants were assessed. PLLA films with added α‐tocopherol and resveratrol showed a yellowish brown color and the lightness was influenced by the presence of the antioxidants. The glass transition and melting temperatures were significantly reduced with the addition of antioxidants while enhanced thermal stability was observed, which could be a benefit and important for processing and production. PLLA films with added antioxidants were slightly more hydrophobic than neat PLLA. The combination effect of plasticizing and enhancement of the elastic modulus with differing concentrations of two antioxidants played a critical role in the mechanical and thermomechanical properties of PLLA films. The melt viscosity of the PLLA films with added antioxidants was substantially higher than that of neat PLLA. The higher melt viscosity and G′(ω) could be an indication of formation of entanglement between PLLA and the two antioxidants. Copyright © 2012 Society of Chemical Industry     

Development of the Analysis of Fecal Stanols in the Oyster Crassostrea gigas and Identification of Fecal Contamination in Shellfish Harvesting Areas

The objective of this work was to study the effects of washing and purification steps on qualitative and quantitative analysis of fecal stanols in the oyster Crassostrea gigas using either single or a combination of lipid purification steps on silica gel or aminopropyl bonded silica gel (NH₂) or a washing step. Among the three analytical pathways compared, the two including water extraction or NH₂ purification did not lead to higher recoveries and decreased repeatabilities of extractions compared to the single purification on silica gel. This latter led to similar recoveries (ca. 80 %) and repeatabilities (ca. 10 %) for both spiked standards (coprostanol and sitostanol). This analytical pathway has been applied to oysters collected in a harvesting area in Brittany (France) where fecal contaminations are important and allowed to quantify eight stanols in oysters. The relative proportions of fecal stanols of these oysters were combined with principal component analysis in order to investigate the usefulness of their stanol fingerprints to record a fecal contamination and to distinguish its source between human, porcine and bovine contaminations. Oysters non-fecally contaminated by Escherichia coli did not present specific stanol fingerprints while oysters fecally contaminated had a bovine fingerprint, suggesting a contamination of these samples by bovine sources. As a consequence, the method developed here allows the use of stanol fingerprints of oysters as a microbial source tracking tool that can be applied to shellfish harvesting areas subjected to fecal contaminations in order to identify the different sources of contamination and improve watershed management.