文星花园汇龙苑小区综合节能概述

在文星花园汇龙苑住宅小区中,因地制宜应用综合的节能措施,取得了良好的社会效益和经济效益.     

土钉支护在路堤深基坑中的应用

有一土质较好的地铁隧道和敞开段交界处的深基坑工程,因不允许有水平支撑及两侧有高速公路而不能采用桩体围护.介绍了采用土钉支护体系的设计和施工的体验,并指出经济、施工快速简便的土钉墙支护的适用务件.     

程序变波长-高效液相色谱法同时测定果蔬中9种防腐剂

建立了柑橘、黄瓜、白菜、西红柿四种果蔬中9种防腐剂（对羟基苯甲酸甲酯、对羟基苯甲酸乙酯、对羟基苯甲酸丙酯、对羟基苯甲酸丁酯、对羟基苯甲酸异丙酯、对羟基苯甲酸异丁酯、乙萘酚、乙氧基喹、4-苯基苯酚）的程序变波长-高效液相色谱同时测定方法。样品采用乙醚提取,液-液萃取净化,以甲醇-水为流动相进行梯度洗脱,经Agilent Eclipse XDB-C18色谱柱（150mm×4.6mm,3.5μm）分离,程序化变波长检测,外标法定量。结果表明,9种防腐剂在0.5¹00.0mg/L范围内线性关系良好（r≥0.9991）;在50、200、500μg/kg三个添加水平下平均回收率为70.7%¹19.5%,相对标准偏差（RSD）为0.9%⁹.9%。该方法简便快速、准确可靠,适用于果蔬中多种防腐剂残留的同时测定。      

超高压处理对牛初乳中IgG活性的影响

牛初乳中富含营养物质和多种活性成分,尤其是IgG的含量特别高。以产犊后48h内的牛初乳为原料,研究不同的超高压处理条件对牛初乳中IgG活性的影响,采用ELISA方法检测经过超高压处理后样品中IgG的活性。结果显示,在压力500MPa、温度35℃、时间20min条件下处理牛初乳,其IgG活性提高率达到最大。      

典型物体表面温度的测量及初步计算

热特性模拟广泛应用于电力、消防、工业、医疗、安防等国民经济及军事部门,而验证是模拟软件优化发展过程中重要的一个环节;对于热特性模拟软件来说,一般通过实验验证对软件进行验证。为了有效快速地进行验证,将地面物体简单处理为CUBI及立方体模型。放置于各实验模型物体表面特征位置（面中心、中线）的热电偶可获得物体的实测温度数据,模拟软件所需的某些输入参数可以通过风速仪等相关仪器获得。通过对实验及计算数据的比较验证了该模拟软件的精确度基本符合要求,并且还发现了在模拟软件中应该注意的一些问题。     

白藜芦醇对小鼠脾淋巴细胞免疫调节活性的影响

研究了白藜芦醇（Res）对小鼠脾淋巴细胞免疫调节活性的影响。无菌分离小鼠脾,制备淋巴细胞,并用含10%胎牛血清的RPMI 1640培养,在培养液中分别加入刀豆蛋白A（Con A）、脂多糖（LPS）以及5、10、20、40μg/m L不同浓度的Res,经过不同时间培养后,采用MTT法检测淋巴细胞的增殖,ELISA法检测淋巴细胞分泌IL-4和IL-12的水平,流式细胞仪检测淋巴细胞的细胞周期和细胞膜表面标志。结果表明:Res能够促进小鼠脾淋巴细胞增殖,能与Con A协同促进小鼠脾淋巴细胞增殖,不能与LPS协同促进小鼠脾淋巴细胞增殖;能增强IL-4和IL-12的分泌;能提高小鼠脾淋巴细胞CD4+/CD8+的比值;能提高脾淋巴细胞周期中细胞进入S期细胞的百分率。综上,Res能增强小鼠脾淋巴细胞的免疫调节活性。      

不同干燥方法对酸枣叶中核苷类、氨基酸类及黄酮类成分的影响

为研究不同干燥方法对酸枣叶干燥过程中内在质量的影响,采用UPLC-TQ MS法和HPLC法分别建立酸枣叶中20种核苷类和23种氨基酸类,以及黄酮类成分芦丁的分析方法,并探索不同干燥方法对酸枣叶中上述组分组成及含量的影响。研究结果表明本研究所建立的分析方法简便、准确可靠;所有样品均富含核苷类、游离氨基酸类及黄酮类成分芦丁,其中核苷类成分以磷酸化产物如胞苷-5’-单磷酸、腺苷-5’-单磷酸、鸟苷-5’-单磷酸含量相对较高,游离氨基酸类成分以脯氨酸、酪氨酸、γ-氨基丁酸含量相对较高;且所有干燥加工后样品其上述成分含量均总体高于新鲜样品。依据各样品中所测定成分的含量,采用TOPSIS分析法,对不同干燥方法干燥样品的内在质量进行了综合评价,结果显示以自然晒干样品和40℃热风干燥样品品质较优,而新鲜样品及70℃热风干燥样品品质较差。以上研究结果为酸枣叶产地干燥加工过程适宜干燥工艺的确定提供了数据支撑,也为叶类药材的干燥加工提供了借鉴。      

Expression Patterns and Gonadotropin Regulation of the TGF-β II Receptor (Bmpr2) during Ovarian Development in the Ricefield Eel Monopterus albus

Bmpr2 plays a central role in the regulation of reproductive development in mammals, but its role during ovarian development in fish is still unclear. To ascertain the function of bmpr2 in ovarian development in the ricefield eel, we isolated and characterized the bmpr2 cDNA sequence; the localization of Bmpr2 protein was determined by immunohistochemical staining; and the expression patterns of bmpr2 in ovarian tissue incubated with FSH and hCG in vitro were analyzed. The full-length bmpr2 cDNA was 3311 bp, with 1061 amino acids encoded. Compared to other tissues, bmpr2 was abundantly expressed in the ovary and highly expressed in the early yolk accumulation (EV) stages of the ovary. In addition, a positive signal for Bmpr2 was detected in the cytoplasm of oocytes in primary growth (PG) and EV stages. In vitro, the expression level of gdf9, the ligand of bmpr2, in the 10 ng/mL FSH treatment group was significantly higher after incubation for 4 h than after incubation for different durations. However, bmpr2 expression in the 10 ng/mL FSH treatment group at 2 h, 4 h and 10 h was significantly lower. Importantly, the expression level of bmpr2 and gdf9 in the 100 IU/mL hCG group had similar changes that were significantly decreased at 4 h and 10 h. In summary, Bmpr2 might play a pivotal role in ovarian growth in the ricefield eel, and these results provide a better understanding of the function of bmpr2 in ovarian development and the basic data for further exploration of the regulatory mechanism of gdf9 in oocyte development.     

The SARS-CoV-2 Delta-Omicron Recombinant Lineage (XD) Exhibits Immune-Escape Properties Similar to the Omicron (BA.1) Variant

Recently, a recombinant SARS-CoV-2 lineage, XD, emerged that harbors a spike gene that is largely derived from the Omicron variant BA.1 in the genetic background of the Delta variant. This finding raised concerns that the recombinant virus might exhibit altered biological properties as compared to the parental viruses and might pose an elevated threat to human health. Here, using pseudotyped particles, we show that ACE2 binding and cell tropism of XD mimics that of BA.1. Further, XD and BA.1 displayed comparable sensitivity to neutralization by antibodies induced upon vaccination with BNT162b2/Comirnaty (BNT) or BNT vaccination followed by breakthrough infection. Our findings reveal important biological commonalities between XD and Omicron BA.1 host cell entry and its inhibition by antibodies.     

Genetic Dissection of Phosphorus Use Efficiency and Genotype-by-Environment Interaction in Maize

Genotype-by-environment interaction (G-by-E) is a common but potentially problematic phenomenon in plant breeding. In this study, we investigated the genotypic performance and two measures of plasticity on a phenotypic and genetic level by assessing 234 maize doubled haploid lines from six populations for 15 traits in seven macro-environments with a focus on varying soil phosphorus levels. It was found intergenic regions contributed the most to the variation of phenotypic linear plasticity. For 15 traits, 124 and 31 quantitative trait loci (QTL) were identified for genotypic performance and phenotypic plasticity, respectively. Further, some genes associated with phosphorus use efficiency, such as Zm00001eb117170, Zm00001eb258520, and Zm00001eb265410, encode small ubiquitin-like modifier E3 ligase were identified. By significantly testing the main effect and G-by-E effect, 38 main QTL and 17 interaction QTL were identified, respectively, in which MQTL38 contained the gene Zm00001eb374120, and its effect was related to phosphorus concentration in the soil, the lower the concentration, the greater the effect. Differences in the size and sign of the QTL effect in multiple environments could account for G-by-E. At last, the superiority of G-by-E in genomic selection was observed. In summary, our findings will provide theoretical guidance for breeding P-efficient and broadly adaptable varieties.