Investigation of partial discharge mechanism by simultaneous measurement of current waveform and light emission

We observed current pulse waveforms of partial discharge (PD) in SF₆ gas so as to investigate the PD mechanism. We also measured light intensity and light emission image of PD simultaneously under different conditions of applies voltage and SF₆ gas pressure. From these experiments, we found that the “double-peak current waveform” appeared at high pressure and high voltage conditions. We also analyzed the mutual correlation of waveforms between a single current and the light emission. Moreover, we obtained experimental evidence of filmentlike light image appearing at the PD tip under the same condition with double-peak current waveform. From the electric field analysis around the needle electrode tip, we believe that the filamentlike light image expands beyond the critical electric field of SF₆ gas. Thus, we concluded that these current waveforms with double peaks showed evidence of leader-type PD, leading to breakdown. Finally, we could point out that leader-type PD should be distinguished and measured for the diagnosis of GIS insulation performance. © 1999 Scripta Technica, Electr Eng Jpn, 129(4): 58–65, 1999     

The effect of ZDDP in CVT fluid on increasing the traction capacity of belt-drive continuously variable transmissions

Belt-drive continuously variable transmissions are already being increasingly used in Europe and Japan for small vehicles, but midsize vehicles will require greater torque capacity. This paper discusses the effect of ZDDP as an additive on increasing the traction obtained at the sliding interfaces between the elements and pulleys of a belt-drive continuously variable transmission (B-CVT). This effect stems from the higher friction coefficient at the sliding interfaces. Friction coefficients were measured in rig tests in which test pieces cut from actual CVT elements were slid against a disc in different types of CVT fluid (CVTF). After the tests, the adsorbed films on the specimen surfaces were investigated with electron probe microanalysis (EPMA), and compared with pieces cut from elements subjected to a high torque test conducted with the B-CVT. The experimental results indicated that the higher friction coefficient at the sliding interfaces was due to the shearing of the tough adsorbed film. The film was blue in colour, and consisted mainly of zinc precipitated from the ZDDP additive.     

Does the Rainbow Trout Ovarian Fluid Promote the Spermatozoon on Its Way to the Egg?

The fertilization of freshwater fish occurs in an environment that may negatively affect the gametes; therefore, the specific mechanisms triggering the encounters of gametes would be highly expedient. The egg and ovarian fluid are likely the major sources of these triggers, which we confirmed here for rainbow trout (Oncorhynchus mykiss). The ovarian fluid affected significantly spermatozoa performance: it supported high velocity for a longer period and changed the motility pattern from tumbling in water to straightforward moving in the ovarian fluid. Rainbow trout ovarian fluid induced a trapping chemotaxis-like effect on activated male gametes, and this effect depended on the properties of the activating medium. The interaction of the spermatozoa with the attracting agents was accompanied by the “turn-and-run” behavior involving asymmetric flagellar beating and Ca²⁺ concentration bursts in the bent flagellum segment, which are characteristic of the chemotactic response. Ovarian fluid created the optimal environment for rainbow trout spermatozoa performance, and the individual peculiarities of the egg (ovarian fluid)–sperm interaction reflect the specific features of the spawning process in this species.     

An unsteady flow structure on a heated rotating disk under mixed convection

A flow field under mixed convection on a heated rotating disk has been measured using an ultrasonic velocity profiler (UVP). The measured velocity field is a spatio‐temporal one as a function of radial coordinates and time. The objective of this paper is to clarify the vortex structure caused by the instability between buoyancy and centrifugal force. The vortex appears under typical conditions of Reynolds numbers and Grashof numbers and it moves toward the outside of the disk. This behavior can be classified into two patterns. The size of the vortex structure decreases with an increasing Reynolds number and increases with the Grashof number. The traveling velocity of the vortex increases with the Grashof number. Moreover, it decreases with an increasing Reynolds number in spite of increasing centrifugal force. According to these results, the region dominated by natural, forced, and mixed convection is classified in the relationship between Reynolds and Grashof numbers. © 2005 Wiley Periodicals, Inc. Heat Trans Asian Res, 34(6): 407–418, 2005; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/htj.20074     

Characterization of Class V DyP-Type Peroxidase SaDyP1 from Streptomyces avermitilis and Evaluation of SaDyPs Expression in Mycelium

DyP-type peroxidases are a family of heme peroxidases named for their ability to degrade persistent anthraquinone dyes. DyP-type peroxidases are subclassified into three classes: classes P, I and V. Based on its genome sequence, Streptomyces avermitilis, eubacteria, has two genes presumed to encode class V DyP-type peroxidases and two class I genes. We have previously shown that ectopically expressed SaDyP2, a member of class V, indeed has the characteristics of a DyP-type peroxidase. In this study, we analyzed SaDyP1, a member of the same class V as SaDyP2. SaDyP1 showed high amino acid sequence identity to SaDyP2, retaining a conserved GXXDG motif and catalytic aspartate. SaDyP1 degraded anthraquinone dyes, which are specific substrates of DyP-type peroxidases but not azo dyes. In addition to such substrate specificity, SaDyP1 showed other features of DyP-type peroxidases, such as low optimal pH. Furthermore, immunoblotting using an anti-SaDyP2 polyclonal antibody revealed that SaDyP1 and/or SaDyP2 is expressed in mycelia of wild-type S. avermitilis.     

Simultaneous rice bran oil stabilization and extraction using sub-critical water medium

Sub-critical water technique was used for simultaneous inactivation of lipase enzyme existing in rice bran and extraction of its oil in order to obtain the stabilized edible rice bran oil. Sub-critical water treatment was carried out in the temperature range between 120 and 240 °C for 10 and/or 20 min residence time in a batch reactor. The quality of the extracted oil was evaluated with respect to its total free fatty acids concentration over a 12 week period, and compared with the oil obtained by conventional extraction methods. Without sub-critical water treatment, the concentration of total free fatty acids in the rice bran significantly increased from 5.6% to 36.0%. In contrast, no increase was observed in the total free fatty acids concentration in the samples treated by sub-critical water. Experimental evidence showed that total free fatty acids concentration increased somewhat in the oils treated by conventional methods. Considering no change was observed in total free fatty acid concentration in the treated oils by sub-critical water, it was found that sub-critical water not only could efficiently extract oil from rice bran in a short residence time but also completely stabilized the extracted oil. Oil extraction yields generally increased with increases in sub-critical water treatment temperature and residence time. The highest extraction yield of oil was 249 (mg/g dry matter) obtained at 240 °C and 10 min residence time. Oil extraction by sub-critical water could be conducted in a very short residence time (10 and/or 20 min). Also, the kinetics of free fatty acids formation in untreated rice bran was investigated and developed which successfully describes the concentration of total free fatty acids in the course of rice bran storage.     

Sub-critical water treatment of rice bran to produce valuable materials

The target of this study is application of sub-critical water as a green solvent for hydrolysis of rice bran and extraction of its oil in order to obtain value-added materials. Experiments were carried out at temperatures ranging from 100 to 360 °C with 5 min residence time in a batch reactor. Four phases were isolated after reaction: hexane-soluble, acetone-soluble, water-soluble, and solid residue phases. Rice bran oil was successfully and efficiently obtained by sub-critical water extraction. Significant increases of total organic carbon (TOC) and total nitrogen (TN) in the water phase were also observed, because polysaccharides and proteins (generally bio-macro polymers) in rice bran were hydrolysed by sub-critical water. The highest yields of TOC and TN were 140 and 13 mg/g dry matter, respectively. As a result, varieties of compounds, such as amino acids, organic acids, and water-soluble saccharides, were identified in the water phase. In particular, significant amounts of water-soluble sugars (maximum yield of total sugars was nearly 190 mg/g dry matter) proved that sub-critical water was a promising medium for dissolution of biomass in water. Acetone-soluble contents were attributed to tar, carbonized biomass, and in general, water- and hexane-insoluble compounds. Solid residue consisted of mainly un-reacted rice bran and insoluble inorganic compounds.     

Characteristic long-chain fatty acid of Pleurocybella porrigens

As part of an investigation on the chemical constituents and contaminants of the basidiomycete Pleurocybella porrigens (Japanese name: Sugihiratake), we analyzed the UV-detected constituents of this mushroom using HPLC. One of the major UV peaks detected was isolated and identified as a-eleostearic acid, a long-chain fatty acid with a conjugated triene moiety, based on the results of spectroscopic methods. alpha-Eleostearic acid was concluded to be a characteristic fatty acid of P. porrigens, because it was not detected in eight other edible mushrooms examined. Free long-chain fatty acids in P. porrigens and other edible mushrooms were analyzed by HPLC after derivatization with acidic 2-nitrophenylhydrazine hydrochloride. Oleic acid was the main fatty acid in P. porrigens, and saturated long-chain fatty acids such as linoleic acid, palmitic acid, and stearic acid, together with a-eleostearic acid, were also detected.