Production of hydrogen via methane reforming using atmospheric pressure microwave plasma

In this paper, results of hydrogen production via methane reforming in the atmospheric pressure microwave plasma are presented. A waveguide-based nozzleless cylinder-type microwave plasma source (MPS) was used to convert methane into hydrogen. Important advantages of the presented waveguide-based nozzleless cylinder-type MPS are: stable operation in various gases (including air) at high flow rates, no need for a cooling system, and impedance matching. The plasma generation was stabilized by an additional swirled nitrogen flow (50 or 100 l min⁻¹). The methane flow rate was up to 175 l min⁻¹. The absorbed microwave power could be changed from 3000 to 5000 W. The hydrogen production rate and the corresponding energy efficiency in the presented methane reforming by the waveguide-based nozzleless cylinder-type MPS were up to 255 g[H₂] h⁻¹ and 85 g[H₂] kWh⁻¹, respectively. These parameters are better than those typical of the conventional methods of hydrogen production (steam reforming of methane and water electrolysis).     

Effect of Antibiotic Amphotericin B Combinations with Selected 1,3,4-Thiadiazole Derivatives on RPTECs in an In Vitro Model

4-(5-methyl-1,3,4-thiadiazole-2-yl) benzene-1,3-diol (C1) and 4-[5-(naphthalen-1-ylmethyl)-1,3,4-thiadiazol-2-yl] benzene1,3-diol (NTBD) are representative derivatives of the thiadiazole group, with a high antimycotic potential and minimal toxicity against normal human fibroblast cells. The present study has proved its ability to synergize with the antifungal activity of AmB. The aim of this work was to evaluate the cytotoxic effects of C1 or NTBD, alone or in combination with AmB, on human renal proximal tubule epithelial cells (RPTECs) in vitro. Cell viability was assessed with the MTT assay. Flow cytometry and spectrofluorimetric techniques were used to assess the type of cell death and production of reactive oxygen species (ROS), respectively. The ELISA assay was performed to measure the caspase-2, -3, and -9 activity. ATR-FTIR spectroscopy was used to evaluate biomolecular changes in RPTECs induced by the tested formulas. The combinations of C1/NTBD and AmB did not exert a strong inhibitory effect on the viability/growth of kidney cells, as evidenced by the negligible changes in the apoptotic/necrotic rate and caspase activity, compared to the control cells. Both NTBD and C1 displayed stronger anti-oxidant activity when combined with AmB. The relatively low nephrotoxicity of the thiadiazole derivative combinations and the protective activity against AmB-induced oxidative stress may indicate their potential use in the therapy of fungal infections.     

Computational Pipeline for Reference-Free Comparative Analysis of RNA 3D Structures Applied to SARS-CoV-2 UTR Models

RNA is a unique biomolecule that is involved in a variety of fundamental biological functions, all of which depend solely on its structure and dynamics. Since the experimental determination of crystal RNA structures is laborious, computational 3D structure prediction methods are experiencing an ongoing and thriving development. Such methods can lead to many models; thus, it is necessary to build comparisons and extract common structural motifs for further medical or biological studies. Here, we introduce a computational pipeline dedicated to reference-free high-throughput comparative analysis of 3D RNA structures. We show its application in the RNA-Puzzles challenge, in which five participating groups attempted to predict the three-dimensional structures of 5′- and 3′-untranslated regions (UTRs) of the SARS-CoV-2 genome. We report the results of this puzzle and discuss the structural motifs obtained from the analysis. All simulated models and tools incorporated into the pipeline are open to scientific and academic use.     

Optimal design of vibration-isolation systems by means of a numerical simulation

Journal of Mechanical Science and Technology - In this paper the computational methodology for evaluating vibro-isolation properties of the vibration reduction systems is discussed. The proposed...     

Transfer of fibroblast sheets cultured on thermoresponsive dishes with membranes

In cell or tissue engineering, it is essential to develop a support for cell-to-cell adhesion, which leads to the generation of cell sheets connected by extracellular matrix. Such supports must be hydrophobic and should result in a detachable cell sheet. A thermoresponsive support that enables the cultured cell sheet to detach using only a change in temperature could be an interesting alternative in regenerative medicine. The aim of this study was to evaluate plates covered with thermoresponsive polymers as supports for the formation of fibroblast sheets and to develop a damage-free procedure for cell sheet transfer with the use of membranes as transfer tools. Human skin fibroblasts were seeded on supports coated with a thermoresponsive polymer: commercial UpCell? dishes (NUNC?) coated with thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) and dishes coated with thermoresponsive poly(tri(ethylene glycol) monoethyl ether methacrylate) (P(TEGMA-EE)). Confluent fibroblast sheets were effectively cultured and harvested from both commercial PNIPAM-coated dishes and laboratory P(TEGMA-EE)-coated dishes. To transfer a detached cell sheet, two membranes, Immobilon-P^® and SUPRATHEL^®, were examined. The use of SUPRATHEL for relocating the cell sheets opens a new possibility for the clinical treatment of wounds. This study established the background for implementing thermoresponsive supports for transplanting in vitro cultured fibroblasts.     

Analytical modelling of multiservice switching networks with multiservice sources and resource management mechanisms

In this paper, we presents an analytical link capacity and outage performance analysis of downlink multiuser diversity (MUD) in multiple-input multiple-output (MIMO) system employing maximal-ratio combining (MRC) with transmit antenna selection (TAS) in the presence of imperfect channel state information (CSI) due to feedback delay over Rayleigh fading channels. The unified achievable analysis is appropriate for MUD–MIMO with TAS/MRC systems in which effective output signal-to-noise ratio (SNR) is specified as highest order statistic of chi-square distribution. Based on this framework, the closed-form channel capacity and outage probability expressions are examined for the MUD–MIMO exploiting TAS/MRC with normalized SNR based scheduling in heterogeneous wireless networks. Further, we derive approximate upper bound capacity as well as capacity at high SNR and low SNR region under delayed feedback CSI. The upper and lower bound of outage probability under delayed feedback CSI is also evaluated. Thereafter the impact of feedback delay and antenna structures with significance on the consideration of MUD on the performance of the system has been analyzed.     

Chemical composition,amino acid and fatty acid contents,and mineral concentrations of European beaver (<Emphasis Type="Italic">Castor fiber</Emphasis> L.) meat

The present investigation was designed to determine the meat nutritional profile of European beaver (Castor fiber L.). The proximate composition, energy value, amino acids composition, chemical scores for indispensable amino acids, fatty acids composition and mineral concentrations were determined. In 100 g of meat the content for moisture ranged from 75.42 to 77.32 g, for protein from 20.21 to 22.33 g, for fat from 0.66 to 2.44 and for ash from 1.12 to 1.24 g. The Nutritional Quality Index for muscle protein was 9. The indispensable amino acids (IAA) at the highest content were lysine and leucine (1.85 and 1.65 g 100 g^?1). The average percentage of IAA was 45% and their chemical scores amounted to more than 100. The total percentages of trans-, saturated (SFA), mono, and polyunsaturated (PUFA) fatty acids were 2.25, 32.25, 18.83 and 49.08%. The ratio of PUFA/SFA and n-6/n-3 PUFA were 1.56 and 5.58. The cholesterol content averaged 55.10 mg 100 g^?1. The muscle tissue of beavers contained favourable amount of minerals, particularly Fe and heme Fe (64.49 and 45.60 mg kg^?1), Zn and Cu (45.07 and 0.98 mg kg^?1), as well a low level of Na (461.9 mg kg^?1). Summing up, beavers are a valuable nutritional meat source with a desirable chemical composition and a low calorific value and can constitute a healthy alternative food as advantageous for human health and coherent with diet recommendations.     

Diesel engine evaluation of a nonionic sunflower oil-aqueous ethanol microemulsion

A nonionic sunflower oil-aqueous ethanol microemulsion was formulated, characterized and evaluated as a fuel in a direct injection, turbocharged, intercooled, 4-cylinder Allis-Chalmers diesel engine during a 200 hr EMA cycle laboratory screening endurance test. Differences in engine operation between a baseline Phillips 2D reference fuel and the experimental fuel were observed. The major problem experienced while operating with the microemulsion was an incomplete combustion process at low-load engine operation. Significant lubricating oil dilution was observed initially, followed by an abnormal increase in the viscosity of the lubricative oil. Heavier carbon residue on the piston lands, in the piston ring grooves and in the intake ports was noted. In addition, premature injection-nozzle deterioration (sticking of the needle) was experienced. At present, the sunflower oil-aqueous ethanol microemulsion studied cannot be recommended for long-term use in a direct-injection diesel engine, but further modifications in formulation may produce acceptable sunflower oil microemulsions as alternative diesel fuels. Presented at the AOCS meeting, Chicago, IL, May 1983.     

MIP model for efficient dimensioning of real-world FTTH trees

We propose a mixed-integer programming (MIP) formulation for a problem of optimal dimensioning of equipment of the fiber-to-the-home optical access network (FTTH-OAN). The optimization aims at minimizing capital expenditures (CAPEX) related to deployment of the FTTH-OAN, i.e., the cost of active equipment (OLTs and OLT cards), cost of passive equipment (optical splitters, cables, fibers, fibers terminations, splices, closures), cost of site preparations (building, cabinets), and cost of necessary labor (duct preparation and rollout of cables). The formulation has been implemented and validated. General results based on selected real-world scale numerical experiments are presented.